Engineering biofuel tolerance in non-native producing microorganisms

Jin, Hu; Chen, Lei; Wang, Jiangxin; Zhang, Weiwen

doi:10.1016/j.biotechadv.2014.02.001

Cited by 66 publications

(39 citation statements)

References 125 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This topic has been explored from the viewpoint of bioremediation of hydrocarbon spills and contamination, biocatalysis using whole microbes in two-phase solvent systems, and the production of solvent-like compounds. Several recent and thoughtful reviews [3,4,9,[13][14][15][16] and comprehensive systems biology studies [17][18][19][20][21][22][23][24][25][26][27] focus on evaluating solvent tolerance across numerous bacterial hosts (also see Box 1). Although a given solvent will elicit specific stress responses, some generalizable trends are now established.…”

Section: Mechanisms Of Solvent Toxicity In Bacteriamentioning

confidence: 99%

“…Mechanisms that may address the toxicity upstream of intracellular damage include maintenance of membrane integrity during solvent accumulation. Membrane lipid modifications via cis-trans isomerases [44], desaturases [16], epoxidases [45], and increased cyclopropyl groups [32] have been used to alter membrane fluidity and increase tolerance. Export pumps that lower the accumulation of the toxic final product inside the cell are especially effective in imparting tolerance and are discussed in greater detail in later sections.…”

Section: Reviewmentioning

confidence: 99%

See 1 more Smart Citation

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Mukhopadhyay

2015

Trends in Microbiology

203

117

View full text Add to dashboard Cite

During microbial production of solvent-like compounds, such as advanced biofuels and bulk chemicals, accumulation of the final product can negatively impact the cultivation of the host microbe and limit the production levels. Consequently, improving solvent tolerance is becoming an essential aspect of engineering microbial production strains. Mechanisms ranging from chaperones to transcriptional factors have been used to obtain solvent-tolerant strains. However, alleviating growth inhibition does not invariably result in increased production. Transporters specifically have emerged as a powerful category of proteins that bestow tolerance and often improve production but are difficult targets for cellular expression. Here we review strain engineering, primarily as it pertains to bacterial solvent tolerance, and the benefits and challenges associated with the expression of membrane-localized transporters in improving solvent tolerance and production.

show abstract

Section: Mechanisms Of Solvent Toxicity In Bacteriamentioning

confidence: 99%

Section: Reviewmentioning

confidence: 99%

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Mukhopadhyay

2015

Trends in Microbiology

203

117

View full text Add to dashboard Cite

show abstract

“…Previous analyses of stress responses to exogenous biofuels in E. coli (Dunlop, 2011; Wang et al, 2013a; Jin et al, 2014) and cyanobacteria (Liu et al, 2012; Qiao et al, 2012; Tian et al, 2013) using conventional methodologies showed that both general stress responses such as up-regulation of heat shock proteins and membrane modification, and possible biofuel-specific responses can be induced by individual biofuel stress. To further decipher metabolic responses using a network-based approach, we first constructed a protein co-expression network using proteomic datasets to determine the general stress responses that were commonly responsive to both biofuels stress and environmental perturbations.…”

Section: Resultsmentioning

confidence: 99%

“…Structures and components of the cell wall are known to be affected by solvents/biofuels (Nicolaou et al, 2010; Dunlop, 2011; Jin et al, 2014). Early analysis of cell morphology found visible cell aggregation under biofuel treatments (Liu et al, 2012; Qiao et al, 2012; Tian et al, 2013), while the aggregation was not obvious under environmental stress (Huang et al, 2013; Qiao et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…Recent efforts have led to successful production of various biofuels in engineered cyanobacterial cells, such as ethanol (Deng and Coleman, 1999), butanol and isobutanol (Atsumi et al, 2009), alkanes (Choi and Lee, 2013), and biodiesel (Da Ros et al, 2013). However, the current biofuel productivity in the cyanobacterial systems is several orders of magnitude lower than their native producing microbes (Jin et al, 2014). In addition to ongoing efforts to optimize the existing pathways and to discover and construct novel pathways, one option to achieve high productivity is to improve cellular tolerance to toxic biofuel products synthesized by the cyanobacterial hosts (Dunlop, 2011; Zingaro and Papoutsakis, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protein Network Signatures Associated with Exogenous Biofuels Treatments in Cyanobacterium Synechocystis sp. PCC 6803

Pei

Chen

Wang

et al. 2014

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Although recognized as a promising microbial cell factory for producing biofuels, current productivity in cyanobacterial systems is low. To make the processes economically feasible, one of the hurdles, which need to be overcome is the low tolerance of hosts to toxic biofuels. Meanwhile, little information is available regarding the cellular responses to biofuels stress in cyanobacteria, which makes it challenging for tolerance engineering. Using large proteomic datasets of Synechocystis under various biofuels stress and environmental perturbation, a protein co-expression network was first constructed and then combined with the experimentally determined protein–protein interaction network. Proteins with statistically higher topological overlap in the integrated network were identified as common responsive proteins to both biofuels stress and environmental perturbations. In addition, a weighted gene co-expression network analysis was performed to distinguish unique responses to biofuels from those to environmental perturbations and to uncover metabolic modules and proteins uniquely associated with biofuels stress. The results showed that biofuel-specific proteins and modules were enriched in several functional categories, including photosynthesis, carbon fixation, and amino acid metabolism, which may represent potential key signatures for biofuels stress responses in Synechocystis. Network-based analysis allowed determination of the responses specifically related to biofuels stress, and the results constituted an important knowledge foundation for tolerance engineering against biofuels in Synechocystis.

show abstract

New Energy Crops for Biofuel Production

Jin

et al. 2015

Handbook of Clean Energy Systems

View full text Add to dashboard Cite

The Sun is sending an enormous amount of light energy to the Earth that is converted and stored as bioenergy in biomass via photosynthesis. Energy in biomass is enough for human energy consumption including ingested food energy, and the question is how to efficiently extract the energy from biomass for different applications. This article describes the development of the first‐, second‐, and third‐generations of biofuels from different biomass materials. Definition and category of energy crops are given. Authors have provided in detail how to generate new energy crops and described their status quo . The article analyzes the potentials of different new energy crops with an emphasis on economic benefits, energy efficiency ( EE ), bottlenecks, and impact on agriculture and environment. A deep discussion on the future barriers in the development of the third‐generation biofuels has been carried out. Good and promising candidate crops in the second‐ and third‐generation biofuels have been pointed out. Even the drawbacks of new energy crops have been realistically analyzed. The future directions in the development of new energy crops to meet human consumptions have been suggested. Finally, potential applications of new energy crops for the first‐, second‐, and third‐generations of biofuels have been discussed.

show abstract

Engineering biofuel tolerance in non-native producing microorganisms

Cited by 66 publications

References 125 publications

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Tolerance engineering in bacteria for the production of advanced biofuels and chemicals

Protein Network Signatures Associated with Exogenous Biofuels Treatments in Cyanobacterium Synechocystis sp. PCC 6803

New Energy Crops for Biofuel Production

Contact Info

Product

Resources

About