Engineering Biosynthesis Mechanisms for Diversifying Polyhydroxyalkanoates

Chen, Guoqiang; Hajnal, Ivan; Wu, Hong; Lv, Li

doi:10.1016/j.tibtech.2015.07.007

Cited by 120 publications

(69 citation statements)

References 77 publications

(70 reference statements)

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“…In contrast to mcl‐PHA fermentation, the production of PHB by C. necator is performed on an industrial scale using another accumulation metabolic pathway . This model was appropriately calibrated for PHB production by C. necator grown on glucose during exponential growth phase of the batch culture, resulting in a parameter set having narrow confidence intervals (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…Sodium octanoate (sodium caprylate) and glucose were used as carbon and energy sources for the cultivation of P. putida GPo1 and C. necator , respectively, whereas ammonium sulfate was used as a nitrogen source for both bacterial cultures. Pseudomonas putida GPo1 synthesizes mcl‐PHAs by octanoate oxidation through the β‐oxidation cycle (Pathway II), whereas C. necator synthesizes PHB from glucose using the Pathway I …”

Section: Methodsmentioning

confidence: 99%

“…Thus, the parameters had a biochemical meaning and the same designation for both fermentations, but the estimated values were different as expected. The exceptions are the a , b , c and d parameters of the observed product yield coefficient on substrate (

Y_{P false/ S_{1}}

) because these fermentations are unrelated biochemical systems . In these studies, the fermentations were maintained at 30 °C and pH 7.0 and were not limited by dissolved oxygen levels.…”

Section: Methodsmentioning

confidence: 99%

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A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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BACKGROUND Poly(hydroxyalkanoates) (PHAs) are biodegradable polymers that can replace conventional plastics, but microbial production of PHAs must be optimized for commercial success. The aim of this study is to provide a simple mathematical model based on previous studies in the literature to represent the production of PHAs in chemostat and batch cultures by Pseudomonas putida GPo1 (ATCC 29347) and Cupriavidus necator (DSM 545) grown on octanoate and glucose, respectively. RESULTS Kinetic and stoichiometric equations, dependent on the specific growth rate of residual biomass (µR), were developed for carbon‐ and nitrogen‐limiting growth conditions. The parameters have biochemical significance and are independent of the biomass concentration. The results revealed that the Luedeking–Piret model is growth‐associated for both fermentations under carbon limitation. In addition, the PHA content increases with µR. Meanwhile, the PHA content is amplified under nitrogen limitation but decreases as μR increases. Also, the Luedeking–Piret model exhibits a profile that depends on the synthesized polyhydroxyalkanoate. CONCLUSION This study demonstrates that strategies for the production of PHAs require an in‐depth understanding of the process kinetics. This model gives satisfactory predictions, may be extended to fed batch cultures and may be adapted to other fermentations. © 2018 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Y_{P false/ S_{1}}

) because these fermentations are unrelated biochemical systems . In these studies, the fermentations were maintained at 30 °C and pH 7.0 and were not limited by dissolved oxygen levels.…”

Section: Methodsmentioning

confidence: 99%

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A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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“…Several molecular engineering tools for Halomonas spp. have been developed (Figure A,B) . A constitutive chromosomal expression system based on highly expressed porin protein gene porin and an inducible cassette named T7‐like expression system induced by isopropyl‐β‐ d ‐thiogalactoside (IPTG) via expressing RNA polymerases (RNAP) under a lacI‐controlled Ptac promoter have been exploited .…”

Section: Next‐generation Industrial Biotechnologymentioning

confidence: 99%

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Chen

2019

Biotechnology Journal

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The chemical industry has made a contribution to modern society by providing cost‐competitive products for our daily use. However, it now faces a serious challenge regarding environmental pollutions and greenhouse gas emission. With the rapid development of molecular biology, biochemistry, and synthetic biology, industrial biotechnology has evolved to become more efficient for production of chemicals and materials. However, in contrast to chemical industries, current industrial biotechnology (CIB) is still not competitive for production of chemicals, materials, and biofuels due to their low efficiency and complicated sterilization processes as well as high‐energy consumption. It must be further developed into “next‐generation industrial biotechnology” (NGIB), which is low‐cost mixed substrates based on less freshwater consumption, energy‐saving, and long‐lasting open continuous intelligent processing, overcoming the shortcomings of CIB and transforming the CIB into competitive processes. Contamination‐resistant microorganism as chassis is the key to a successful NGIB, which requires resistance to microbial or phage contaminations, and available tools and methods for metabolic or synthetic biology engineering. This review proposes a list of contamination‐resistant bacteria and takes Halomonas spp. as an example for the production of a variety of products, including polyhydroxyalkanoates under open‐ and continuous‐processing conditions proposed for NGIB.

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“…[307,308] Block copolymers can display structures such as AB diblock, ABC triblock,o r( AB) n repeating multiblocks. [312][313][314][315] Block copolymerization thus opens up an ew area in the production of new macromolecular structures of PHAs that display tuned and improved properties. As an example, C. necator allows the productiono fP (HB-b-HV) block copolymer under certain conditions, and this particular structure prevents polymer aging, which is one of the main issues of P(3HB).…”

Section: Pha Copolymersmentioning

confidence: 99%

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

2018

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Biobased polymers have seen their attractiveness increase in recent decades thanks to the significant development of biorefineries to allow access to a wide variety of biobased building blocks. Polyesters are one of the best examples of the development of biobased polymers because most of them now have their monomers produced from renewable resources and are biodegradable. Currently, these polyesters are mainly produced by using traditional chemical catalysts and harsh conditions, but recently greener pathways with nontoxic enzymes as biocatalysts and mild conditions have shown great potential. Bacterial polyesters, such as poly(hydroxyalkanoate)s (PHA), are the best example of the biotic production of high molar mass polymers. PHAs display a wide variety of macromolecular architectures, which allow a large range of applications. The present contribution aims to provide an overview of recent progress in studies on biobased polyesters, especially those made from short building blocks, synthesized through step-growth polymerization. In addition, some important technical aspects of their syntheses through biotic or abiotic pathways have been detailed.

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Engineering Biosynthesis Mechanisms for Diversifying Polyhydroxyalkanoates

Cited by 120 publications

References 77 publications

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Biotic and Abiotic Synthesis of Renewable Aliphatic Polyesters from Short Building Blocks Obtained from Biotechnology

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