Engineering budding yeast for the production of coumarins from lignin

Zhao, Chen-Hui; Zhang, Ren-Kuan; Qiao, Bin; Li, Bingzhi; Yuan, Ying‐Jin

doi:10.1016/j.bej.2020.107634

Cited by 30 publications

(18 citation statements)

References 40 publications

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“…The study of the other enzymes that allow the production of curcumin using glucose as substrate were not the focus of this study as they are widely used in S. cerevisiae in the construction of other pathways. [26][27][28][29] Moreover, we wanted to study curcumin production without the interference of other enzymes activity. Therefore, the biosynthesis of curcumin by engineered S. cerevisiae was first attempted by introducing two enzymes responsible for the conversion of phenylpropanoid ferulic acid into curcumin, namely the 4CL1 from A. thaliana and CUS from O. sativa.…”

Section: Curcumin Was Produced In S Cerevisiae From Supplemented Feru...mentioning

confidence: 99%

Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae

Rainha

Rodrigues

Faria

et al. 2021

Biotechnology Journal

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Background and Goal: Curcumin is a polyphenolic compound found in Curcuma longa.This bioactive molecule has several reported health-benefit effects, being the anticarcinogenic activity among the most promising ones. However, curcumin extraction from natural sources is hampered by impure products obtained from harsh chemicals and limited by plant seasonality and high prices. Therefore, curcumin heterologous production emerged as an interesting alternative. Escherichia coli has been explored as chassis but the implementation of the pathway in Saccharomyces cerevisiae can have several advantages, including its generally regarded as safe status. Hence, S. cerevisiae was engineered for the first time to produce curcumin from its precursor ferulic acid. Methods and Results:The enzymes 4-coumarate-CoA ligase (4CL1) from Arabidopsis thaliana or feruloyl-CoA synthetase (FerA) from Pseudomonas paucimobilis and type III polyketide synthases (PKSs) from Oryza sativa or C. longa were expressed in BY4741 strain. To avoid ferulic acid deviation, the gene FDC1 coding a ferulic acid decarboxylase was deleted. The maximum curcumin titer was obtained with FerA combined with C. longa PKSs (2.7 mg L −1 ).Conclusions and Implications: Up to our knowledge, this is the first work reporting the expression of a feruloyl-CoA synthase and also curcuminoid biosynthetic enzymes in S. cerevisiae, and consequently, curcumin production.

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Section: Curcumin Was Produced In S Cerevisiae From Supplemented Feru...mentioning

confidence: 99%

Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae

Rainha

Rodrigues

Faria

et al. 2021

Biotechnology Journal

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“…These methods can involve chemical and physical approaches, such as hydrolysis and extraction, or bacterial action [ 31 , 32 , 33 , 34 , 35 ]. An alternative method could be that of resorting to synthetic biology to valorize lignin and biosynthesized coumarins [ 36 ]. Another bio-source of FA could be agro-industrial waste, which represents a large, cheap and available source of chemicals [ 29 , 37 ].…”

Section: Introductionmentioning

confidence: 99%

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

et al. 2021

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Bio-based monomers represent the future market for polymer chemistry, since the political economics of different states promote green ventures toward more sustainable materials and processes. Industrial pulp and paper processing represent a large market that could advance the use of by-products to avoid waste production and reduce pollution. Lignin represents the most available side product that can be used to produce a bio-based monomer. This review is concentrated on the possibility of using bio-based monomer derivates from pulp and the paper industry for UV-curing processing. UV-curing represents the new frontier for thermoset production, allowing a fast reaction cure, less energy demand, and the elimination of solvent. The growing demand for new monomers increases research in the environmental field to substitute for petroleum-based products. This review provides an overview of the main monomers and relative families of compounds derived from industrial processes that are suitable for UV-curing. Particular focus is given to the developments reached in the last few years concerning lignin, rosin and terpenes and the related possible applications of these in UV-curing chemistry.

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“…The synthesis of coumarins can also be produced from aromatic compounds derived from lignin by pretreatment [ 37 , 38 ]. For example, two major monomers, p -coumaric acid and ferulic acid, can be generated in alkaline hydrolysate from pretreatment and used for the biosynthesis of scopoletin.…”

Section: Biosynthesis Pathway Of Scopoletinmentioning

confidence: 99%

“…By fusing F6'H1 from Ipomoea batatas with glutathione S-transferase (GTS) and expressing 4CL from Oryza sativa in E. coli , the yield of scopoletin increased to 79.5 mg/L using ferulic acid as a substrate [ 34 ]. Ferulic acid was transformed into scopoletin in S. cerevisiae using the heterologous expression [ 38 ]. Fusion expression of feruloyl coenzyme A 6'-hydroxylase (F6'H1) from Arabidopsis thaliana and 4-coumarate coenzyme A ligase (4CL) from Petroselinum crispum significant improved the production of scopoletin in S. cerevisiae .…”

Section: Regulation Of Scopoletin Biosynthesismentioning

confidence: 99%

Advances in biosynthesis of scopoletin

Liu

et al. 2022

Microb Cell Fact

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Scopoletin is a typical example of coumarins, which can be produced in plants. Scopoletin acts as a precursor for pharmaceutical and health care products, and also possesses promising biological properties, including antibacterial, anti-tubercular, anti-hypertensive, anti-inflammatory, anti-diabetic, and anti-hyperuricemic activity. Despite the potential benefits, the production of scopoletin using traditional extraction processes from plants is unsatisfactory. In recent years, synthetic biology has developed rapidly and enabled the effective construction of microbial cell factories for production of high value-added chemicals. Herein, this review summarizes the progress of scopoletin biosynthesis in artificial microbial cell factories. The two main pathways of scopoletin biosynthesis are summarized firstly. Then, synthetic microbial cell factories are reviewed as an attractive improvement strategy for biosynthesis. Emerging techniques in synthetic biology and metabolic engineering are introduced as innovative tools for the efficient synthesis of scopoletin. This review showcases the potential of biosynthesis of scopoletin in artificial microbial cell factories.

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Engineering budding yeast for the production of coumarins from lignin

Cited by 30 publications

References 40 publications

Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae

Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae

UV-Curable Bio-Based Polymers Derived from Industrial Pulp and Paper Processes

Advances in biosynthesis of scopoletin

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