Engineering cartilage tissue based on cartilage-derived extracellular matrix cECM/PCL hybrid nanofibrous scaffold

Feng, Bei; Ji, Tian-Ji; Wang, Xingang; Fu, Wei; Ye, Lincai; Zhang, Hao; Li, Fen

doi:10.1016/j.matdes.2020.108773

Cited by 64 publications

(35 citation statements)

References 46 publications

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“…Similarly, when implanted in immunodeficient NSG mice, the deposition of collagenous fibers in both cell-free and cell-laden scaffolds suggest that b-TPUe can allow in vivo GAGs and collagenous fibre production as well as PCL. Thus, it can be stated that b-TPUe polymer scaffolds showed good in vivo ECM deposition confirming the integration of b-TPUe within the host's tissue(76).…”

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confidence: 53%

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Validation of the 1,4‐butanediolthermoplastic polyurethane as a novel material for3Dbioprinting applications

Chocarro‐Wrona

Vicente

Antich

et al. 2020

Bioengineering & Transla Med

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Background Tissue engineering (TE) seeks to fabricate implants that mimic the mechanical strength, structure, and composition of native tissues. Cartilage TE requires the development of functional personalized implants with cartilage-like mechanical properties capable of sustaining high loadbearing environments to integrate into the surrounding tissue of the cartilage defect. Objective In this study we evaluated the novel 1,4-Butanediol thermoplastic polyurethane elastomer (b-TPUe) derivative filament as a 3D bioprinting material with application in cartilage TE. Methods The mechanical behaviour of b-TPUe in terms of friction and elasticity were examined and compared with human articular cartilage, PCL, and PLA. Moreover, infrapatellar fat pad-derived human mesenchymal stem cells (MSCs) were bioprinted together with scaffolds. In vitro cytotoxicity, proliferative potential, cell viability, and chondrogenic differentiation were analysed by Alamar blue assay, SEM, confocal microscopy, and RT-qPCR. Moreover, in vivo biocompatibility and host integration were analysed. Results This article is protected by copyright. All rights reserved. b-TPUe demonstrated a much closer compression and shear behaviour to native cartilage than PCL and PLA, as well as closer tribological properties to cartilage. Moreover, b-TPUe bioprinted scaffolds were able to maintain proper proliferative potential, cell viability, and supported MSCs chondrogenesis. Finally, in vivo studies revealed no toxic effects 21 days after scaffolds implantation, extracellular matrix (ECM) deposition and integration within the surrounding tissue. Conclusions This is the first study that validates the biocompatibility of b-TPUe for 3D bioprinting. Our findings indicate that this biomaterial can be exploited for the automated biofabrication of artificial tissues with tailorable mechanical properties including the great potential for cartilage TE applications.

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confidence: 53%

“…Thus, it can be stated that b-TPUe polymer scaffolds showed good in vivo ECM deposition confirming the integration of b-TPUe within the host's tissue. 75…”

Section: Proliferation and Viability Of Mscs Cultured In B-tpue Bioprmentioning

confidence: 99%

Validation of the 1,4‐butanediolthermoplastic polyurethane as a novel material for3Dbioprinting applications

Chocarro‐Wrona

Vicente

Antich

et al. 2020

Bioengineering & Transla Med

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“…The IR spectrum of PCL powder in Fig. 1/2 has its characteristic absorption peak of NHCOO-at 1725 cm -1 [15]. In Fig.…”

Section: The Results Of Ftir Spectroscopic Analysismentioning

confidence: 97%

PCL Based CIP-Loaded Double-Layer Films Deposited by Low-Electron Beam Dispersion Method and its Antibacterial Properties

Zhou

et al. 2020

Eurasian Chem. Tech. J.

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In this paper, low-electron beam dispersion (EBD) method is used to prepare a kind of double-layer films on different substrates. The bottom layer is a mechanically stirred mixture of the degradable polycaprolactone (PCL) and ciprofloxacin (CIP), and the top layer is polyurethane (PU) film. The molecular structure, chemical composition and morphology of the double-layer films were investigated by FTIR, XPS and SEM. The results showed that the surfaces of the double-layer films are uniform and the thicknesses can reach micron level. In addition, the two layers are well bonded. Then the films were sliced and immersed in PBS solution, and the time-dependent variable was used to analyze the kinetic slow-release behavior of CIP in the double-layer films by agar diffusion antibacterial experiments. It can be seen that sustained release time of CIP in the double-layer films can be up to 7 days, which is due to fact that the upper PU film working as a sealing layer helps to realize the drug slow-release. Based on the above research, the comprehensive performance of the films with the composition of PCL:CIP/PU=1:1/1 is the best.

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“…It was demonstrated that decellularizing ECM can play a remarkable role in cellular activities in cartilage TE [ 109 ]. Decellularization of ECM effectively removes components of cells and DNA, but not entirely, which the remaining components such as cell membrane particles can affect chondrogenic differentiation ability [ 110 ].…”

Section: Bioactive Inksmentioning

confidence: 99%

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

Bakhtiary

Liu

Ghorbani

2021

Gels

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Nowadays, a prevalent joint disease affecting both cartilage and subchondral bone is osteoarthritis. Osteochondral tissue, a complex tissue unit, exhibited limited self-renewal potential. Furthermore, its gradient properties, including mechanical property, bio-compositions, and cellular behaviors, present a challenge in repairing and regenerating damaged osteochondral tissues. Here, tissue engineering and translational medicine development using bioprinting technology provided a promising strategy for osteochondral tissue repair. In this regard, personalized stratified scaffolds, which play an influential role in osteochondral regeneration, can provide potential treatment options in early-stage osteoarthritis to delay or avoid the use of joint replacements. Accordingly, bioactive scaffolds with possible integration with surrounding tissue and controlling inflammatory responses have promising future tissue engineering perspectives. This minireview focuses on introducing biologically active inks for bioprinting the hierarchical scaffolds, containing growth factors and bioactive materials for 3D printing of regenerative osteochondral substitutes.

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Engineering cartilage tissue based on cartilage-derived extracellular matrix cECM/PCL hybrid nanofibrous scaffold

Cited by 64 publications

References 46 publications

Validation of the 1,4‐butanediolthermoplastic polyurethane as a novel material for3Dbioprinting applications

Validation of the 1,4‐butanediolthermoplastic polyurethane as a novel material for3Dbioprinting applications

PCL Based CIP-Loaded Double-Layer Films Deposited by Low-Electron Beam Dispersion Method and its Antibacterial Properties

Bioactive Inks Development for Osteochondral Tissue Engineering: A Mini-Review

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