Engineering cell matrix interactions in assembled polyelectrolyte fiber hydrogels for mesenchymal stem cell chondrogenesis

Raghothaman, Deepak; Leong, Meng Fatt; Lim, Tze Chiun; Toh, Jerry K. C.; Wan, Andrew C.A.; Yang, Zheng; Lee, Eng Hin

doi:10.1016/j.biomaterials.2013.12.008

Cited by 52 publications

(44 citation statements)

References 48 publications

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“…[37][38][39][40] In the early stage of chondrogenesis, integrin-mediated FAK activation is essential for cellular condensation, 41,42 and Raghothaman et al suggested the importance of coupling of integrin mediated cell-matrix interactions and N-cadherin/ b-catenin mediated downstream signaling events in the MSC chondrogenesis. 43 In the present study, GSEA showed that the focal adhesion-related pathway and integrin-mediated pathway were enhanced in the PAMPS Culture. These results suggest that integrin played an important role in the adhesion of ATDC5 cells to the PAMPS gel surface.…”

Section: Discussionsupporting

confidence: 54%

SyntheticPAMPSgel activatesBMP/Smad signaling pathway inATDC5 cells, which plays a significant role in the gel‐induced chondrogenic differentiation

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Kimura

Kitamura

et al. 2015

J Biomedical Materials Res

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Abstract:The purposes of this study were to identify signaling pathways that were specifically activated in ATDC5 cells cultured on poly (2-acrylamido-2-methylpropanesulfonic acid) (PAMPS) gel in insulin-free maintenance medium and to evaluate the significance of the determined signaling pathways in the chondrogenic differentiation induced by this gel. In this study, ATDC5 cells cultured on PAMPS gel using the maintenance medium without insulin (PAMPS Culture) were compared with cells cultured on polystyrene using the differentiation medium containing insulin (PS-I Culture). The microarray analysis, Western blot analysis, and

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Section: Discussionsupporting

confidence: 54%

SyntheticPAMPSgel activatesBMP/Smad signaling pathway inATDC5 cells, which plays a significant role in the gel‐induced chondrogenic differentiation

Goto

Kimura

Kitamura

et al. 2015

J Biomedical Materials Res

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“…Recently, fiber‐based techniques have been widely used as bottom–up scaffolds to form hierarchical structures in numerous tissue engineering applications . Electrospinning, microfluidic extruding, and interface complexation are commonly used techniques to produce microfibers . Compared with other techniques, microfluidic systems, with the ability to handle liquids in the microscale, provide an effective way to flexibly manipulate microfluid and thus produce microscale fibers with various morphologies (e.g., tubular, flat, grooved, porous, and hybrid fibers), without the use of complicated devices or facilities .…”

Section: Introductionmentioning

confidence: 99%

“…Sang‐Hoon Lee et al have adopted a coaxial flow microfluidic device to fabricate NaA‐CS microfibers; however, the filtering procedure, which is to remove undesired aggregated suspension of NaA and CS before spinning, makes the amount of CS component left in the NaA‐CS microfibers uncertain. Wan et al have used interfacial complexation technique to fabricate NaA/CS solid microfibers; however, the diameter of the microfibers fabricated by this method was varied from 10 to 20 μm. Large‐scale cells encapsulation and bulk production of the microfibers remain a bottleneck.…”

Section: Introductionmentioning

confidence: 99%

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Liu

Wang

et al. 2019

J Biomed Mater Res

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Sodium alginate (NaA) has been widely used as microfiber‐based scaffold material. However, Ca‐alginate microfiber might disintegrate in the physiological environment due to the loss of calcium ions, which will limit its long‐term application in tissue engineering. In this work, to enhance the stability of Ca‐alginate microfiber in the physiological environment, an inner chitosan coating was introduced to Ca‐alginate hollow microfiber by one step via a microfluidic device. A more stable composite microfiber with double cross‐linking layers was generated. The stability of the microfiber was studied in the PBS solution (pH 7.4) to identify the coating effect on the hollow structure. The results revealed that chitosan component bonded an NaA layer to form a stable polyelectrolyte complex membrane in the inner wall of the microfiber, which stabilized the hollow region even though the Ca‐alginate shell was disintegrated by PBS solution. In addition, the introduction of chitosan coating improved the inner environment of the low affinity of alginate to cell surfaces and facilitated the cell adhesion and culture in the microfiber. HepG2 cells in the microfibers displayed favorable cell viability and proliferation ability. We believe that this work will lead to the development of innovative methodologies and materials for both cell culture and tissue engineering application. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2527–2536, 2019.

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“…Chondrogenic differentiation of MSCs was induced in 3D pellet cultures as previously described [45,46]. Briefly, 2.5 × 10 5 cells were centrifuged to form pellets and cultured in a chondrogenic differentiation medium containing high-glucose DMEM supplemented with 4 mM proline, 50 μg/mL ascorbic acid, 1% ITS-Premix (Becton-Dickinson, San Jose, CA), 1 mM sodium pyruvate, and 10 − 7 M dexamethasone (Sigma Aldrich, St Louis, MO), for up to 7 or 21 days in the presence of 10 ng/mL of transforming growth factor-β3 (TGFβ3; R&D Systems, Minneapolis, MN).…”

Section: Msc Chondrogenesismentioning

confidence: 99%

Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration

et al. 2020

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Background: The mesenchymal stem cell (MSC) secretome, via the combined actions of its plethora of biologically active factors, is capable of orchestrating the regenerative responses of numerous tissues by both eliciting and amplifying biological responses within recipient cells. MSCs are "environmentally responsive" to local micro-environmental cues and biophysical perturbations, influencing their differentiation as well as secretion of bioactive factors. We have previously shown that exposures of MSCs to pulsed electromagnetic fields (PEMFs) enhanced MSC chondrogenesis. Here, we investigate the influence of PEMF exposure over the paracrine activity of MSCs and its significance to cartilage regeneration.Methods: Conditioned medium (CM) was generated from MSCs subjected to either 3D or 2D culturing platforms, with or without PEMF exposure. The paracrine effects of CM over chondrocytes and MSC chondrogenesis, migration and proliferation, as well as the inflammatory status and induced apoptosis in chondrocytes and MSCs was assessed.Results: We show that benefits of magnetic field stimulation over MSC-derived chondrogenesis can be partly ascribed to its ability to modulate the MSC secretome. MSCs cultured on either 2D or 3D platforms displayed distinct magnetic sensitivities, whereby MSCs grown in 2D or 3D platforms responded most favorably to PEMF exposure at 2 mT and 3 mT amplitudes, respectively. Ten minutes of PEMF exposure was sufficient to substantially augment the chondrogenic potential of MSC-derived CM generated from either platform. Furthermore, PEMF-induced CM was capable of enhancing the migration of chondrocytes and MSCs as well as mitigating cellular inflammation and apoptosis. Conclusions:The findings reported here demonstrate that PEMF stimulation is capable of modulating the paracrine function of MSCs for the enhancement and re-establishment of cartilage regeneration in states of cellular stress. The PEMF-induced modulation of the MSC-derived paracrine function for directed biological responses in recipient cells or tissues has broad clinical and practical ramifications with high translational value across numerous clinical applications.

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Engineering cell matrix interactions in assembled polyelectrolyte fiber hydrogels for mesenchymal stem cell chondrogenesis

Cited by 52 publications

References 48 publications

SyntheticPAMPSgel activatesBMP/Smad signaling pathway inATDC5 cells, which plays a significant role in the gel‐induced chondrogenic differentiation

SyntheticPAMPSgel activatesBMP/Smad signaling pathway inATDC5 cells, which plays a significant role in the gel‐induced chondrogenic differentiation

Simple fabrication of inner chitosan‐coated alginate hollow microfiber with higher stability

Pulsed electromagnetic fields potentiate the paracrine function of mesenchymal stem cells for cartilage regeneration

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