Engineering cellulolytic bacterium <i>Clostridium thermocellum</i> to co‐ferment cellulose‐ and hemicellulose‐derived sugars simultaneously

Xiong, Wei; Reyes, Luis H.; Michener, William E.; Maness, Pin‐Ching; Chou, Katherine

doi:10.1002/bit.26590

Cited by 57 publications

(33 citation statements)

References 42 publications

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“…The latter can be further utilized by the cells and fermented to products of industrial interest (e.g., H 2 , formate, lactate, acetate, ethanol) (Ellis et al, 2012 ; Holwerda et al, 2014 ). Recent advances in genetic modification tools (Tripathi et al, 2010 ; Argyros et al, 2011 ) further enable heterologous expression of new functional pathways (e.g., pentose sugar utilization Xiong et al, 2018 , isobutanol production Lin et al, 2015 ) into the bacterium, making this CBP bacterium an attractive and engineerable platform for biofuels or biochemicals production.…”

Section: Introductionmentioning

confidence: 99%

Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Xiong

Chou

et al. 2018

Front. Microbiol.

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Cellulolytic bacteria have the potential to perform lignocellulose hydrolysis and fermentation simultaneously. The metabolic pathways of these bacteria, therefore, require more comprehensive and quantitative understanding. Using isotope tracer, gas chromatography-mass spectrometry, and metabolic flux modeling, we decipher the metabolic network of Clostridium thermocellum, a model cellulolytic bacterium which represents as an attractive platform for conversion of lignocellulose to dedicated products. We uncover that the Embden–Meyerhof–Parnas (EMP) pathway is the predominant glycolytic route whereas the Entner–Doudoroff (ED) pathway and oxidative pentose phosphate pathway are inactive. We also observe that C. thermocellum's TCA cycle is initiated by both Si- and Re-citrate synthase, and it is disconnected between 2-oxoglutarate and oxaloacetate in the oxidative direction; C. thermocellum uses a citramalate shunt to synthesize isoleucine; and both the one-carbon pathway and the malate shunt are highly active in this bacterium. To gain a quantitative understanding, we further formulate a fluxome map to quantify the metabolic fluxes through central metabolic pathways. This work represents the first global in vivo investigation of the principal carbon metabolism of C. thermocellum. Our results elucidate the unique structure of metabolic network in this cellulolytic bacterium and demonstrate the capability of isotope-assisted metabolite studies in understanding microbial metabolism of industrial interests.

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Section: Introductionmentioning

confidence: 99%

Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Xiong

Chou

et al. 2018

Front. Microbiol.

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“…xylose as compared to cellulose alone (Xiong et al 2018). This study represents an encouraging step towards the use of plant biomass for the synthesis of valuable products.…”

Section: Metabolic Engineering Of Clostridial Strains To Promote H 2 mentioning

confidence: 83%

Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives

Latifi

Avilán

Brugna

2018

Appl Microbiol Biotechnol

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Strictly anaerobic bacteria of the Clostridium genus have attracted great interest as potential cell factories for molecular hydrogen production purposes. In addition to being a useful approach to this process, dark fermentation has the advantage of using the degradation of cheap agricultural residues and industrial wastes for molecular hydrogen production. However, many improvements are still required before large-scale hydrogen production from clostridial metabolism is possible. Here we review the literature on the basic biological processes involved in clostridial hydrogen production, and present the main advances obtained so far in order to enhance the hydrogen productivity, as well as suggesting some possible future prospects.

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“…1). Studies of xylose metabolic engineering in solventogenic Clostridia provide a reference for cellulolytic clostridia used in CBP (Xiong et al, 2018).…”

Section: Integrase-mediated Chromosome Integrationmentioning

confidence: 99%

Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

Wen

Liu

et al. 2019

Microbial Biotechnology

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Summary Butanol is an important bulk chemical, as well as a promising renewable gasoline substitute, that is commonly produced by solventogenic Clostridia. The main cost of cellulosic butanol fermentation is caused by cellulases that are required to saccharify lignocellulose, since solventogenic Clostridia cannot efficiently secrete cellulases. However, cellulolytic Clostridia can natively degrade lignocellulose and produce ethanol, acetate, butyrate and even butanol. Therefore, cellulolytic Clostridia offer an alternative to develop consolidated bioprocessing (CBP), which combines cellulase production, lignocellulose hydrolysis and co‐fermentation of hexose/pentose into butanol in one step. This review focuses on CBP advances for butanol production of cellulolytic Clostridia and various synthetic biotechnologies that drive these advances. Moreover, the efforts to optimize the CBP‐enabling cellulolytic Clostridia chassis are also discussed. These include the development of genetic tools, pentose metabolic engineering and the improvement of butanol tolerance. Designer cellulolytic Clostridia or consortium provide a promising approach and resource to accelerate future CBP for butanol production.

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Engineering cellulolytic bacterium Clostridium thermocellum to co‐ferment cellulose‐ and hemicellulose‐derived sugars simultaneously

Cited by 57 publications

References 42 publications

Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Isotope-Assisted Metabolite Analysis Sheds Light on Central Carbon Metabolism of a Model Cellulolytic Bacterium Clostridium thermocellum

Clostridial whole cell and enzyme systems for hydrogen production: current state and perspectives

Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization

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