Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach

Deb, Dipanwita; Mallick, Nirupama; Bhadoria, P. B. S.

doi:10.1016/j.renene.2020.10.086

Cited by 14 publications

(14 citation statements)

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“…These findings may be attributed to the importance of nitrogen and phosphorus as two main nutrients, without which the cell's metabolic route is diverted, resulting in the accumulation of energy storage molecules [49,50]. While +50% C-CO 3 2− was the optimum condition for the maximum carbohydrate contents of 251.67 and 206 mg L −1 for N. muscorum and A. variabilis, respectively (Figure 3A), our finding was consistent with a previous study that observed improving the total carbohydrate yield and content of A. variabilis to 438.2 mg L −1 and 59.6% CDW, respectively, using 3X NaHCO 3 supplementation [5]. The reason behind such an increase in cellular carbohydrate content under high carbonate levels might be because such conditions induced the synthesis of carbonic anhydrase and bicarbonate transporters that increase the fixed level of CO 2 , which helps to enhance their photosynthetic ability [51,52].…”

Section: Biochemical Compositionsupporting

confidence: 92%

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Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

et al. 2021

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The present study aims to improve biomass and biochemical constituents, especially lipid production of Anabaena variabilis and Nostoc muscorum by formulating an optimal growth condition using various concentrations of nutrients (NO3−, PO43− and CO32−) for biodiesel production. The supplementation of the three nutrients by +50% showed the maximum dry weight and biomass productivity, while the macromolecule contents were varied. The depletion of N-NO3− by 50% N-NO3− showed the maximum lipid yield (146.67 mg L−1) in A. variabilis and the maximum carbohydrate contents (285.33 mg L−1) in N. muscorum with an increase of 35% and 30% over control of the synthetic medium, respectively. However, variation in P-PO43− and C-CO32− showed insignificant improving results for all biochemical compositions in both cyanobacteria. A. variabilis was the superior species for lipid and protein accumulation; however, N. muscorum showed the maximum carbohydrate content. Accordingly, A. variabilis was selected for biodiesel production. In A. variabilis, −50% N-NO3− resulted in 35% higher lipid productivity compared to the control. Furthermore, the fatty acid profile and biodiesel quality-related parameters have improved under this condition. This study has revealed the strategies to improve A. variabilis lipid productivity for biodiesel production for small-scale in vitro application in terms of fuel quality under low nitrate levels.

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Section: Biochemical Compositionsupporting

confidence: 92%

“…Cyanobacteria are an ecologically sound option for biofuel production because of their rapid development rate, higher photosynthetic potential, sustenance in the face of environmental conditions and involvement in global CO 2 mitigation [5]. Cyanobacterial lipids could be one of the promising feedstocks for biodiesel production [6,7].…”

Section: Introductionmentioning

confidence: 99%

Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

et al. 2021

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“…The projected earnings from commercializing all the cyanobacterial products (EPS, SCC, C-PC, PHB, and bioethanol) obtained under a scaled-up scenario of the present approach corresponds to ~ 7.2 million US$. This estimation has been carried out considering the market price of each product 23 , 26 . However, the total production cost would also include the cost of the cultivation set-up.…”

Section: Resultsmentioning

confidence: 99%

“…Control cultures of the nitrogen-fixing cyanobacterium Anabaena variabilis were grown in 100 mL BG-11 medium, modified by eliminating the incorporation of additional nitrogen source 22 . The composition of this modified BG-11 medium has been elaborated in Deb et al 23 The culture room for incubating the cyanobacterial culture was set at a temperature of 25 ± 2 °C, receiving 75 µmole photon m −2 s −1 PAR (14:10 h) light intensity, without carbon dioxide/air sparging. The cultures were subjected to manual shaking on a regular basis (2–3 times a day) to negate their adherence to the flask’s lower surface.…”

Section: Methodsmentioning

confidence: 99%

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Deb

Mallick

Bhadoria

2022

Sci Rep

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The current research work was an innovative approach providing dual advantages of waste bioremediation and an effective biorefinery. The study attempted to exploit wastewater like aqua discharge and solid wastes like poultry litter/cow dung for cyanobacterial cultivation. Aqua discharge appended with 7.5 g L−1 poultry litter turned out as the best combination generating 46% higher carbohydrate yield than BG-11 control. A. variabilis cultivation in this waste-utilized medium also revealed its excellent bioremediation ability. While 100% removal was observed for nitrite, nitrate, and orthophosphate, a respective 74% and 81% reduction was noted for ammonium and total organic carbon. Chemical and biological oxygen demands were also reduced by 90%. This work was also novel in developing a sequential design for the production of bioethanol and co-products like exopolysaccharides, sodium copper chlorophyllin, C-phycocyanin, and poly-β-hydroxybutyrate from the same cyanobacterial biomass. The developed biorefinery implementing the waste-utilized medium was one of its kind, enabling biomass valorization of 61%. Therefore, the present study would provide a leading-edge for tackling the high production costs that limit the practical viability of biorefinery projects. The recyclability of the bioremediated wastewater would not only curtail freshwater usage, the waste disposal concerns would also be mitigated to a great extent.

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“…Anabaena variabilis using RSM. At pH 8.9, MgSO4 (168.8 mg/L) and NaHCO3 (64.3 mg/L), PC yield and content were 54.2 mg/L and 6.3 % dcw, respectively [47].…”

Section: The Effects Of Reactants On Phycocyanin (Pc) Concentrations By Using Response Surface Methodologymentioning

confidence: 98%

Enhancing Phycoerythrin and Phycocyanin Production from Porphyridium cruentum CCALA 415 in Synthetic Wastewater: The Application of Theoretical Methods on Microalgae

Onay

2021

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi

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A stra t: Phycoerythrin (PE) and phycocyanin (PC) are florescent pigments. They have the colorant role in the industry. In this study, production of PE and PC from Porphyridium cruentum were investigated at the various conditions such as different concentrations of municipal wastewater, wavelengths and salicylic acid using Response Surface Methodology-Central Composite Design (RSM-CCD), regression analysis and rstool models. The maximum RSM predicted PE concentration was 29.5 mg/g biomass at 50 % of wastewater, 510 nm of wavelength and 10 µM of salicylic acid. On the other hand, maximum RSM predicted PC concentration was 6.9 mg/g biomass at 50% of wastewater, 680 nm and 40 µM of salicylic acid. According to the ANOVA results, the square effects of the three variables (X1, X2 and X3) were found to be significant for the phycocyanin concentration, while the wastewater and salicylic acid variables (X1 and X3) were found to be important in the Phycoerythrin concentration. In addition to this, the highest PE and PC concentrations were 27.648 and 5.7104 mg/g biomass, respectively, for 50 % of wastewater, 512.5 nm and 47.0833 µM of salicylic acid according to rstool model. In con clusion, the variables such as wastewater, wavelength and salicylic acid can be used for the highest PE and PC concentration by means of RSM-CCD and rstool models and these variables may contribute to the industrial production of the two pigments.

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Engineering culture medium for enhanced carbohydrate accumulation in Anabaena variabilis to stimulate production of bioethanol and other high-value co-products under cyanobacterial refinery approach

Cited by 14 publications

References 60 publications

Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

Influence of Nutrient Manipulation on Growth and Biochemical Constituent in Anabaena variabilis and Nostoc muscorum to Enhance Biodiesel Production

A waste-to-wealth initiative exploiting the potential of Anabaena variabilis for designing an integrated biorefinery

Enhancing Phycoerythrin and Phycocyanin Production from Porphyridium cruentum CCALA 415 in Synthetic Wastewater: The Application of Theoretical Methods on Microalgae

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