Engineering, Differentiation and Harvesting of Human Adipose-Derived Stem Cell Multilayer Cell Sheets

Oliva, Joan; Florentino, Arjie; Bardag‐Gorce, Fawzia; Niihara, Yutaka

doi:10.2217/rme-2018-0053

Cited by 14 publications

(17 citation statements)

References 37 publications

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“…Hamdi showed that grafting cells directly onto a heart damage area improved the heart functionality more, compared to injected single cells in the veins [123]. Different scaffolds can be considered as an option for an organ’s direct grafting of the MSC: Ceramic, collagen, hyaluronan [124] or none [125,126]. The scaffold should play a temporary role in grafting the cells onto the tissue, to let the normal tissue take over the scaffold when it degrades [127].…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Therapeutic Properties of Mesenchymal Stem Cell on Organ Ischemia-Reperfusion Injury

Oliva¹

2019

IJMS

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The shortage of donor organs is a major global concern. Organ failure requires the transplantation of functional organs. Donor’s organs are preserved for variable periods of warm and cold ischemia time, which requires placing them into a preservation device. Ischemia and reperfusion damage the organs, due to the lack of oxygen during the ischemia step, as well as the oxidative stress during the reperfusion step. Different methodologies are developed to prevent or to diminish the level of injuries. Preservation solutions were first developed to maximize cold static preservation, which includes the addition of several chemical compounds. The next chapter of organ preservation comes with the perfusion machine, where mechanical devices provide continuous flow and oxygenation ex vivo to the organs being preserved. In the addition of inhibitors of mitogen-activated protein kinase and inhibitors of the proteasome, mesenchymal stem cells began being used 13 years ago to prevent or diminish the organ’s injuries. Mesenchymal stem cells (e.g., bone marrow stem cells, adipose derived stem cells and umbilical cord stem cells) have proven to be powerful tools in repairing damaged organs. This review will focus upon the use of some bone marrow stem cells, adipose-derived stem cells and umbilical cord stem cells on preventing or decreasing the injuries due to ischemia-reperfusion.

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Section: Discussion and Perspectivesmentioning

confidence: 99%

Therapeutic Properties of Mesenchymal Stem Cell on Organ Ischemia-Reperfusion Injury

Oliva¹

2019

IJMS

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“…In addition to the thermoresponsive surface, other methodologies were developed to have the cell sheets in one piece: collagenase, amniotic membrane, electroresponsive/photoresponsive/pHresponsive/magnetic-responsive surface, and mechanical harvesting [94]. Instead of injecting single isolated cells, we have focused on engineering cell sheet with adipose stromal cells for different reasons, as explained in the introduction [95].…”

Section: Cell Sheet Technologymentioning

confidence: 99%

“…We have developed multilayer cell sheets, using adipose stromal cells [95]. Using specific cell culture conditions, the undifferentiated multilayer adipose stromal cell sheets have been engineered as well as differentiated cell sheets.…”

Section: Cell Sheet Technologymentioning

confidence: 99%

“…In the contrary, autologous stem cell transplantation is limited by the number of cells available, the outcome of the treatment will vary based on the patients, and it will be more expensive than allogeneic transplantation because stem cells from each patient will have to be tested. Using commercially available ASCs, we succeeded to engineer chondrocyte multilayer cell sheets, harvested and characterized after differentiation treatment, as well as osteoblast cell sheets [95]. Bone marrow stem cells were used to engineer osteoblast cells sheets, using a specific scaffold [104].…”

Section: Cell Sheet Technologymentioning

confidence: 99%

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Development of Multilayer Mesenchymal Stem Cell Cell Sheets

Ochiai¹,

Niihara²,

Oliva³

2021

IJTM

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Cell and gene therapies have been developing dramatically over the past decade. To face and adapt to the development of these new therapies, the Food and Drug Administration (FDA) wrote and updated new guidelines from 2016 and keep updating them. Mesenchymal stem cells (MSCs) are the most used cells for treatment, far ahead from the induced pluripotent stem cells (iPSCs), based on registered clinical trials at clinicaltrials.gov. They are widely used because of their differentiation capacity and their anti-inflammatory properties, but some controversies still require clear answers. Additional studies are needed to determine the dosage, the number, and the route of injections (location and transplantation method), and if allogenic MSCs are safe compared to autologous MSC injection, including their long-term effect. In this review, we summarize the research our company is conducting with the adipose stromal cells in engineering cell sheets and their potential application.

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“…A thermo-responsive surface can be used to collect a multilayer cell sheet, which can also be used for single-layer cell sheets. Other methodologies have been used for cell sheet harvesting, such as polyvinylidene di-fluoride membrane or Ethylene diamine tetraacetic acid, only for cell sheets with a strong cell-cell connection [9].…”

Section: Scaffold-free Strategymentioning

confidence: 99%

Scaffold-Free 3-D Cell Sheet Technique Bridges the Gap between 2-D Cell Culture and Animal Models

Alghuwainem

Alshareeda

Al-Sowayan

2019

IJMS

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Various tissue engineering techniques have been created in research spanning two centuries, resulting in new opportunities for growing cells in culture and the creation of 3-D tissue-like constructs. These techniques are classified as scaffold-based and scaffold-free techniques. Cell sheet, as a scaffold-free technique, has attracted research interest in the context of drug discovery and tissue repair, because it provides more predictive data for in vivo testing. It is one of the most promising techniques and has the potential to treat degenerative tissues such as heart, kidneys, and liver. In this paper, we argue the advantages of cell sheets as a scaffold-free approach, compared to other techniques, including scaffold-based and scaffold-free techniques such as the classic systemic injection of cell suspension.

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Engineering, Differentiation and Harvesting of Human Adipose-Derived Stem Cell Multilayer Cell Sheets

Cited by 14 publications

References 37 publications

Therapeutic Properties of Mesenchymal Stem Cell on Organ Ischemia-Reperfusion Injury

Therapeutic Properties of Mesenchymal Stem Cell on Organ Ischemia-Reperfusion Injury

Development of Multilayer Mesenchymal Stem Cell Cell Sheets

Scaffold-Free 3-D Cell Sheet Technique Bridges the Gap between 2-D Cell Culture and Animal Models

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