Engineering DNA on the Surface of Upconversion Nanoparticles for Bioanalysis and Therapeutics

Zhang, Dailiang; Peng, Richard; Liu, Wenfei; Donovan, Michael; Wang, Linlin; Ismail, Ismail; Li, Jin; Li, Juan; Qu, Fengli; Tan, Weihong

doi:10.1021/acsnano.1c08036

Cited by 47 publications

(30 citation statements)

References 142 publications

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“…Despite its great potential for regulation of cellular functions, it remains a great challenge for spatiotemporal control of cellular functions through engineering the cell surface with nucleic acids. For this case, integrating light-controlled DNA switches or other nanomaterials (e.g., upconversion nanoparticles) into the system for engineering cell surfaces may be one feasible direction. − Nevertheless, with more efforts made and nucleic acid technology developed, we believe that nucleic acid-based cell surface engineering will bloom in the near future.…”

Section: Discussionmentioning

confidence: 99%

Nucleic Acid-Based Cell Surface Engineering Strategies and Their Applications

Xiao

Lai

et al. 2022

ACS Appl. Bio Mater.

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The cell membrane is a biological interface regulating the communications between cells and their environment. The ability to functionalize the cell membrane with molecules or nanomaterials allows us to manipulate cellular behaviors and to expand cellular functions. Due to their unique merits of synthetic accessibility, flexible design, and precise programmability, nucleic acids provide an emerging and promising molecular toolkit for cell surface engineering. In this review, the recent progress in nucleic acid-based cell surface engineering are summarized. We first introduce approaches to nucleic acid-based cell surface engineering, including monovalent and polyvalent surface engineering strategies. Then, the biological applications of nucleic acid-based cell surface engineering in biosensing of extracellular microenvironment, programming cell−cell interactions, and mimicking cellular behaviors are reviewed. Finally, we analyze the current challenges existing in this area and discuss the prospects for the future development.

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Section: Discussionmentioning

confidence: 99%

Nucleic Acid-Based Cell Surface Engineering Strategies and Their Applications

Xiao

Lai

et al. 2022

ACS Appl. Bio Mater.

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“…As an emerging optical material, upconversion nanoparticles (UCNPs) consist of a host matrix and lanthanide dopants and can convert the laser energy from long to short wavelength. In recent years, UCNPs have been developed as attractive nanomaterials in imaging and detection because of advantages such as photostability and multiple narrow and well-separated emissions. − The surfaces of positively charged UCNPs can strongly attract negatively charged DNA by electrostatic interaction . In addition, the coordination bonds can be formed between yttrium ions (Y 3+ ) and the phosphate groups in DNA so that DNA chains are able to assemble on the interfaces of UCNPs (Figure A) .…”

Section: Dna Assembly On the Interfaces Of Nanoscale Particlesmentioning

confidence: 99%

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

Yao

Tang

et al. 2022

Acc. Chem. Res.

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Metrics & MoreArticle Recommendations CONSPECTUS: Facing increasing demand for precision medicine, materials chemistry systems for bioanalysis with accurate molecular design, controllable structure, and adjustable biological activity are required. As a genetic biomacromolecule, deoxyribonucleic acid (DNA) is created via precise, efficient, and mild processes in life systems and can in turn precisely regulate life activities. From the perspective of materials chemistry, DNA possesses the characteristics of sequence programmability and can be endowed with customized functions by the rational design of sequences. In recent years, DNA has been considered to be a potential biomaterial for analysis and has been applied in the fields of bioseparation, biosensing, and detection imaging. To further improve the precision of bioanalysis, the supramolecular assembly of DNA on micro/nanointerfaces is an effective strategy to concentrate functional DNA modules, and thus the functions of DNA molecules for bioanalysis can be enriched and enhanced. Moreover, the new modes of DNA supramolecular assembly on micro/nanointerfaces enable the integration of DNA with the introduced components, breaking the restriction of limited functions of DNA materials and achieving more precise regulation and manipulation in bioanalysis. In this Account, we summarize our recent work on DNA supramolecular assembly on micro/nanointerfaces for bioanalysis from two main aspects. In the first part, we describe DNA supramolecular assembly on the interfaces of microscale living cells. The synthesis strategy of DNA is based on rolling-circle amplification (RCA), which generates ultralong DNA strands according to circular DNA templates. The templates can be designed with complementary sequences of functional modules such as aptamers, which allow DNA to specifically bind with cellular interfaces and achieve efficient cell separation. In the second part, we describe DNA supramolecular assembly on the interfaces of nanoscale particles. DNA sequences are designed with functional modules such as targeting, drug loading, and gene expression and then are assembled on interfaces of particles including upconversion nanoparticles (UCNPs), gold nanoparticles (AuNPs), and magnetic nanoparticle (MNPs). The integration of DNA with these functional particles achieves cell manipulation, targeted tumor imaging, and cellular regulation. The processes of interfacial assembly are well controlled, and the functions of the obtained bioanalytical materials can be flexibly regulated. We envision that the work on DNA supramolecular assembly on micro/nanointerfaces will be a typical paradigm for the construction of more bioanalytical materials, which we hope will facilitate the development of precision medicine.

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“…Surface modification of UCNPs with DNA has been largely exploited for bioanalysis and therapeutics. [ 26 ] The surface modification endows the UCNPs with water dispersibility, biocompatibility, and a recognition unit for biodetection, drug loading, or assembly with other NPs. However, one of the main challenges is the synthesis of DNA‐capped UCNPs that are stable in physiological environments while keeping the hybridization functionality of the surface DNA.…”

Section: Introductionmentioning

confidence: 99%

“…Coating with polymers or small molecules that include functional groups (e.g., COOH or NH 2 ) has the potential to combine good shielding of the UCNP with relatively thin shells and keeping the functionality of DNA. [ 37,38 ] Modified (e.g., with NH 2 or SH groups) [ 26 ] DNA can be electrostatically adsorbed or covalently linked to the polymer via EDC/NHS chemistry or thiol‐amine crosslinking. The polymer shell prevents interactions between the UCNP surface and the phosphate backbone of DNA as well as other biological molecules.…”

Section: Introductionmentioning

confidence: 99%

DNA‐Coated Upconversion Nanoparticles for Sensitive Nucleic Acid FRET Biosensing

Francés‐Soriano

Estébanez

Pérez‐Prieto

et al. 2022

Adv Funct Materials

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Despite the significant advancements of developing upconversion nanoparticles (UCNPs) for high performance biosensing and bioimaging, the development of DNA‐functionalized UCNPs with thin coatings, efficient surface passivation, and fully functional DNAs for hybridization sensing in biological media remains extremely challenging. Here, a straightforward concept of labeling DNA to a thin polysulfonate polymer layer on UCNPs is presented. Both UCNPs and DNA preserve their full functionality as demonstrated by Förster resonance energy transfer (FRET) hybridization assays with a Cy3‐conjugated complementary DNA. The important bioanalytical benefits of the UCNP‐DNA nanohybrids are demonstrated by their implementation into rapid and wash‐free microRNA hybridization FRET assays. Using a simple fiber‐coupled USB‐spectrometer and 980 nm diode laser excitation in a 96‐well microplate, ratiometric FRET from UCNP‐to‐Cy3 can quantify miR20a in a 0.01–10 × 10−9 m concentration range with a detection limit of 30 × 10−12 m (4.5 fmol of miR20a), which is more than 20‐fold lower compared to quantum dot‐based FRET assays. The functional thin‐coating DNA‐UCNP nanohybrids have a strong potential for translating UCNPs into advanced DNA‐based biosensing, bioimaging, and high‐throughput and point‐of‐care clinical diagnostics.

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Engineering DNA on the Surface of Upconversion Nanoparticles for Bioanalysis and Therapeutics

Cited by 47 publications

References 142 publications

Nucleic Acid-Based Cell Surface Engineering Strategies and Their Applications

Nucleic Acid-Based Cell Surface Engineering Strategies and Their Applications

DNA Supramolecular Assembly on Micro/Nanointerfaces for Bioanalysis

DNA‐Coated Upconversion Nanoparticles for Sensitive Nucleic Acid FRET Biosensing

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