Engineering DNA scaffolds for delivery of anticancer therapeutics

Sun, Wujin; Gu, Zhen

doi:10.1039/c4bm00459k

Cited by 60 publications

(24 citation statements)

References 77 publications

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“…Therefore, developing the different delivery systems which can target cancer cells either actively or passively, thereby reducing adverse side effects to the normal tissues, is desirable [67]. Recently, a variety of delivery systems with improved therapeutic efficacy have developed due to the understanding of the tumor biology and developing versatile materials with increased bioavailability such as polymers [68][69][70], lipids [71,72], polymeric hydrogels [73,74], inorganic carriers [75], and biomacromolecular scaffolds [76]. The entrance of the nanotechnology in the field of the clinical therapeutics has had grate impact during the last two decades.…”

Section: Alginate Applicationsmentioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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Hydrogels are a three-dimensional and crosslinked network of hydrophilic polymers. They can absorb a large amount of water or biological fluids, which leads to their swelling while maintaining their 3D structure without dissolving (Zhu and Marchant, Expert Rev Med Devices 8:607-626, 2011). Among the numerous polymers which have been utilized for the preparation of the hydrogels, polysaccharides have gained more attention in the area of pharmaceutics; Sodium alginate is a non-toxic, biocompatible, and biodegradable polysaccharide with several unique physicochemical properties for which has used as delivery vehicles for drugs (Kumar Giri et al., Curr Drug Deliv 9:539-555, 2012). Owing to their high-water content and resembling the natural soft tissue, hydrogels were studied a lot as a scaffold. The formation of hydrogels can occur by interactions of the anionic alginates with multivalent inorganic cations through a typical ionotropic gelation method. However, those applications require the control of some properties such as mechanical stiffness, swelling, degradation, cell attachment, and binding or release of bioactive molecules by using the chemical or physical modifications of the alginate hydrogel. In the current review, an overview of alginate hydrogels and their properties will be presented as well as the methods of producing alginate hydrogels. In the next section of the present review paper, the application of the alginate hydrogels will be defined as drug delivery vehicles for chemotherapeutic agents. The recent advances in the application of the alginate-based hydrogels will be describe later as a wound dressing and bioink in 3D bioprinting.

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Section: Alginate Applicationsmentioning

confidence: 99%

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

et al. 2020

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“…Future implementation of the delivery vehicles may focus on attaching cell-specific targeting ligands, [22] engineering the environmentally responsive release of CRISPR-Cas9, [14,23] modifying the sequence of the DNAN Cs to incorporate multiple sgRNAs for multiplex editing,o re mploying the DNAN Cs or packaged DNAs equences as templates for homology-directed repair.T he same NC architecture could also be used to incorporate other functional DNA-binding proteins,s uch as transcription factors,z inc-finger nucleases, and TALE nucleases,a sw ell as other functional or proteincoding RNAs.T he potential immunogenicity associated with DNAn anoclews should be further investigated for clinical translation. [24]…”

Section: Angewandte Chemiementioning

confidence: 99%

Self‐Assembled DNA Nanoclews for the Efficient Delivery of CRISPR–Cas9 for Genome Editing

et al. 2015

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CRISPR-Cas9 represents ap romising platform for genome editing, yet means for its safe and efficient delivery remain to be fully realized. Anovel vehicle that simultaneously delivers the Cas9 protein and single guide RNA( sgRNA) is based on DNAn anoclews,y arn-like DNAn anoparticles that are synthesized by rolling circle amplification. The biologically inspired vehicles were efficiently loaded with Cas9/sgRNA complexes and delivered the complexes to the nuclei of human cells,thus enabling targeted gene disruption while maintaining cell viability.E diting was most efficient when the DNA nanoclew sequence and the sgRNAg uide sequence were partially complementary,o ffering ad esign rule for enhancing delivery.Overall, this strategy provides aversatile method that could be adapted for delivering other DNA-binding proteins or functional nucleic acids.

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“…The potential immunogenicity associated with DNA NCs should be further investigated for clinical translation. [24] …”

mentioning

confidence: 99%

Self‐Assembled DNA Nanoclews for the Efficient Delivery of CRISPR–Cas9 for Genome Editing

et al. 2015

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CRISPR-Cas9 represents a promising technology for genome editing, yet means of safe and efficient delivery remain to be fully realized. Here, we report a novel delivery vehicle to deliver the Cas9 protein and single-guide RNA simultaneously based on DNA nanoclews, yarn-like DNA nanoparticles synthesized by rolling circle amplification. The bio-inspired vehicles efficiently loaded Cas9/single-guide RNA complexes and delivered the complexes to the nuclei of human cells, allowing targeted gene disruptions while maintaining cell viability. Editing was most efficient when the DNA nanoclew sequence and the sgRNA guide sequence were partially complementary, offering a design rule for enhancing delivery. Overall, this strategy provides a versatile platform that could be adapted for delivering other DNA-binding proteins or for functional nucleic acids.

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Engineering DNA scaffolds for delivery of anticancer therapeutics

Cited by 60 publications

References 77 publications

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Alginate-based hydrogels as drug delivery vehicles in cancer treatment and their applications in wound dressing and 3D bioprinting

Self‐Assembled DNA Nanoclews for the Efficient Delivery of CRISPR–Cas9 for Genome Editing

Self‐Assembled DNA Nanoclews for the Efficient Delivery of CRISPR–Cas9 for Genome Editing

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