Engineering ear-shaped cartilage using electrospun fibrous membranes of gelatin/polycaprolactone

Xue, Jixin; Feng, Bei; Zheng, Rui; Yang, Lu; Zhou, Guangdong; Liu, Wei; Cao, Yilin; Zhang, Yanzhong; Zhang, Wen Jie

doi:10.1016/j.biomaterials.2012.12.011

Cited by 145 publications

(105 citation statements)

References 33 publications

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“…Gelatin, a natural biopolymer derived from partial hydrolysis of native collagen, has many integrin-binding sites for cell adhesion and differentiation [21]. PCL-gelatin hybrid material, a new biomaterial with good biocompatibility and improved mechanical, physical, and chemical properties [22], has been successfully used in various tissue engineering applications [23][24][25]. However, phase separation between PCL and gelatin adversely affect both the electrospinning process and the resultant fiber performance.…”

Section: Introductionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

330

219

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Email address: sharell@126.com (R. Shi). AbstractInfection is the major reason for guided tissue regeneration/guided bone regeneration (GTR/GBR) membrane failure in clinical application. In this work, we developed GTR/GBR membranes with localized drug delivery function to prevent infection by electrospinning of poly(ε-caprolactone) (PCL) and gelatin blended with metronidazole (MNA). Acetic acid (HAc) was introduced to improve the miscibility of PCL and gelatin to fabricate homogeneous hybrid nanofiber membranes. The effects of the addition of HAc and the MNA content (0,1,5,10,20,30, and 40 wt.% of polymer) on the properties of the membranes were investigated. The membranes showed good mechanical properties, appropriate biodegradation rate and barrier function. The controlled and sustained release of MNA from the membranes significantly prevented the colonization of anaerobic bacteria. Cells could adhere to and proliferate on the membranes without cytotoxicity until the MNA content reached 30%.Subcutaneous implantation in rabbits for 8 months demonstrated that MNA-loaded membranes evoked a less severe inflammatory response depending on the dose of MNA than bare membranes. The biodegradation time of the membranes was appropriate for tissue regeneration. These results indicated the potential for using MNA-loaded PCL/gelatin electrospun membranes as anti-infective GTR/GBR membranes to optimize clinical application of GTR/GBR strategies.

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Section: Introductionmentioning

confidence: 99%

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Xue

Liu³

et al. 2014

Biomaterials

330

219

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“…Few groups have worked with methods that can apply patient-specific morphologies to engineered ear constructs, 19,20 resulting in generic ears that must be surgically manipulated to reproduce particular aesthetics. Additionally, the maintenance of ear morphology following implantation often requires mechanical assistance, either through external stenting over the skin 14 or internal plastic 21 or metal 10,[22][23][24] support that is included with the implant. Our group has previously applied digital photogrammetry and computer-assisted design/computer-aided manufacturing (CAD/CAM) techniques to fabricate molds replicating juvenile patient ears, a novel technique to recreate specific ear morphology.…”

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confidence: 99%

Long-Term Morphological and Microarchitectural Stability of Tissue-Engineered, Patient-Specific AuriclesIn Vivo

Cohen

Hooper

Puetzer

et al. 2016

Tissue Engineering Part A

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“…. Porém, uma das principais áreas de estudo ou aplicação da PCL na medicina é no desenvolvimento de scaffolds para regeneração tecidual em ossos [162][163][164] , cartilagens [165][166][167] , vasos sanguíneos 168,169 , peles 170,172 e nervos 173,174 . Para estas aplicações são frequentemente descritas as técnicas de copolimerização ou de modificação química da superfície do scaffold, que mantêm as propriedades do material, alterando apenas sua superfície, para melhorar a biocompatibilidade 175 .…”

Section: Policaprolactonaunclassified

Biopolímeros: aplicações farmacêutica e biomédica

et al. 2016

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____________________________________________________________________________Resumo: O estudo e desenvolvimento de polímeros para aplicação nas áreas biomédica e farmacêutica têm aumentado devido às suas propriedades peculiares que contribuem para a melhoria da qualidade de vida, como por exemplo, os polímeros usados em medicina regenerativa e sistemas de liberação de fármacos. O desenvolvimento de novos materiais baseados em polímeros depende desde os métodos de síntese, extração ou composição desses materiais até os estudos da influência de suas estruturas e propriedades em aplicações específicas, entre outras. Esta revisão descreve o uso de alguns de polímeros novos e convencionais com potencial para aplicação nas áreas farmacêutica e biomédica, enfatizando as principais propriedades que os tornam aplicáveis. Palavras-chave: biopolímero, medicina regenerativa, liberação de fármacos ____________________________________________________________________________ Abstract:The study and development of polymers for pharmaceutical and biomedical use has been increasing due to their peculiar properties that contribute for the improvement of the life quality, such as the polymers used in regenerative medicine and in drug release systems. The development of new polymer based materials and its composites depends on several steps, such as the synthesis approach, the extraction, the composition, the influence of their properties on the specific applications, and others. This review describes the use of conventional and new polymers with potential application in pharmaceutical and biomedical fields, highlighting the properties that allow them to be useful for such purposes.

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Engineering ear-shaped cartilage using electrospun fibrous membranes of gelatin/polycaprolactone

Cited by 145 publications

References 33 publications

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Drug loaded homogeneous electrospun PCL/gelatin hybrid nanofiber structures for anti-infective tissue regeneration membranes

Long-Term Morphological and Microarchitectural Stability of Tissue-Engineered, Patient-Specific AuriclesIn Vivo

Biopolímeros: aplicações farmacêutica e biomédica

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