Engineering Escherichia coli to Combat Cancer

Mycoplasma pneumoniae Genome Editing Based on Oligo Recombineering and Cas9-Mediated Counterselection

“…O ne main aim of synthetic biology is to design microorganisms with novel capabilities that could be interesting for a huge variety of applications, such as green chemistry, 1,2 bioremediation, 3 and microbial therapies. 4 As an integrative discipline, synthetic biology aims to bring an engineering perspective to the design of new living forms. 5 Following this rationale, designer organisms should be based on a genomic backbone (i.e., chassis), ideally depleted of most of the functions irrelevant to its programmed purpose, with additional modules that can be plugged into this chassis to add novel functionalities.…”