Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

Chen, Xiangbin; Yin, Jun; Ye, Jianwen; Zhang, Haoqian; Che, Xuemei; Ma, Yiming; Li, Mengyi; Wu, Lin

doi:10.1016/j.biortech.2017.07.149

Cited by 130 publications

(59 citation statements)

References 39 publications

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“…Chen et al, 2017;Erkorkmaz, Kırtel, Ateş Duru, & Toksoy Öner, 2018;Fu et al, 2014;Jiang et al, 2017;Yue et al, 2014). Chen et al, 2017). Since PHA is synthesized under nutrient limitations (S. Y.…”

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confidence: 99%

“…with some successes (X. Chen et al, 2017;Erkorkmaz, Kırtel, Ateş Duru, & Toksoy Öner, 2018;Fu et al, 2014;Jiang et al, 2017;Yue et al, 2014). The continuous processes demand continuous biomass collection and product purification, leading to increased process complexity (Brethauer & Wyman, 2010;Lynd, Grethlein, & Wolkin, 1989).…”

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confidence: 99%

“…Lee, 1996;Steinbüchel, 1991), it is challenging to balance cell growth and PHA accumulation in a continuous process (X. Chen et al, 2017). While the fed-batch process requires repeated fermentation preparations and inoculations, it has relatively low efficiency in terms of energy, manpower, and equipment utilization (Brethauer & Wyman, 2010).…”

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confidence: 99%

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Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Chen

Qiao

Shuai

et al. 2019

Biotech & Bioengineering

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Halomonas has been developed as a platform for the next generation industrial biotechnology allowing open and nonsterile growth without microbial contamination under a high-salt concentration and alkali pH. To reduce downstream cost associated with continuous centrifugation and salt containing wastewater treatment, Halomonas campaniensis strain LS21 was engineered to become self-flocculating by knocking out an etf operon encoding two subunits of an electron transferring flavoprotein in the predicted electron transfer chain. Self-flocculation could be attributed to the decrease of the surface charge and increase of the cellular hydrophobicity resulted from deleted etf. A wastewaterless fermentation strategy based on the self-flocculating H. campaniensis was developed for growth and the production of poly-3-hydroxybutyrate (PHB) as an example. Most microbial cells flocculated and precipitated to the bottom of the bioreactor within 1 min after stopping the aeration and agitation. The supernatant can be used again without sterilization or inoculation for the growth of the next batch after collecting the precipitated cell mass. The wastewaterless process was conducted for four runs without generating wastewater. PHB accumulation by the self-flocculent strain was enhanced via promoter and ribosome binding site optimizations, the productivities of cell dry weight and PHB were increased from 0.45 and 0.18 g·L −1 ·hr −1 for the batch process compared to 0.82 and 0.33 g·L −1 ·hr −1 for the wastewaterless continuous process, respectively. This has clearly demonstrated the advantages of the wastewaterless process in that it not only reduces wastewater but also increases cell growth and product formation efficiency in a given period of time. K E Y W O R D S continuous process, etf, flocculation, Halomonas campaniensis, next generation industry biotechnology, NGIB, PHB

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Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Chen

Qiao

Shuai

et al. 2019

Biotech & Bioengineering

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“…Chen's group designed an engineering Halomonas bluephagenesis TD01 with genome optimization to produce P(3HB‐ co ‐4HB) with cell dry weight of more than 80 g L −1 . P(3HB‐ co ‐4HB) obtained by the engineered bacteria has excellent fracture growth rate and elasticity . Subsequently, Chen's group further designed halophilic phage TD01 with four genes that can encode succinate hemialdehyde dehydrogenase, 2‐oxy glutarate decarboxylase, 4‐hydroxybutyrate coenzyme A (CoA) transferase, and 4‐hydroxybutyrate dehydrogenase.…”

Section: Synthesis Of Polyhydroxyalkanoatesmentioning

confidence: 99%

Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

Luo

Liu

et al. 2019

Biotechnology Journal

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In recent years, naturally biodegradable polyhydroxyalkanoate (PHA) monopolymers have become focus of public attentions due to their good biocompatibility. However, due to its poor mechanical properties, high production costs, and limited functionality, its applications in materials, energy, and biomedical applications are greatly limited. In recent years, researchers have found that PHA copolymers have better thermal properties, mechanical processability, and physicochemical properties relative to their homopolymers. This review summarizes the synthesis of PHA copolymers by the latest biosynthetic and chemical modification methods. The modified PHA copolymer could greatly reduce the production cost with elevated mechanical or physicochemical properties, which can further meet the practical needs of various fields. This review further summarizes the broad applications of modified PHA copolymers in biomedical applications, which might shred lights on their commercial applications.

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“…For example, Halomonas nitroreducens isolated from the surface water of Las Coloradas solar salt pond (Mexico), was able to produce up to 33 w% PHB (Cervantes-Uc et al, 2014). Halomonas bluephagenesis was originally isolated from the hypersaline Aydingol Lake (Xinjiang Province, China) and showed high PHB production (61 w% PHB) during growth in a non-sterile bioreactor (Chen et al, 2017). Genetically transformed H. bluephagenesis was capable of enhanced PHB production in large scale bioreactors (Chen and Jang, 2018).…”

Section: Current and Potential Uses Of Halotolerant And Halophilic Bamentioning

confidence: 99%

The Saline Aquatic Systems As ‘Natural Reservoirs’ For Microorganisms with Current and Potential Applications

Buda¹,

Bulzu²,

Cristea³

et al. 2019

Air and Water Components of the Environment Conference

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Saline aquatic systems include inland and marine-derived lakes, solar salterns, or temporary inland saline ponds. Despite their high salinity, these ecosystems are inhabited by diverse microbial communities driving full biogeochemical cycling of main elements. To date, numerous salt-loving (or 'halophilic') environmental strains with metabolic traits of applicative interest belonging to all Domains of Life (Bacteria, Archaea, Eukarya) have been isolated from saline aquatic systems. The aim of the present work is to briefly review some of the most relevant achievements in the bioprospecting of saline aquatic systems for compounds and metabolic capabilities with potential uses in the environmental, industrial, and food biotechnologies. Additionally, the successful stories of current commercial exploitations of a few halotolerant (e.g., the bacterium Halomonas elongata), halophilic (e.g., the green algae Dunaliella sp.), and haloalkaliphilic microbes (e.g., the sulfur-oxidizing bacteria of genus Thioalkalivibrio sp.) are overviewed.

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Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate)

Cited by 130 publications

References 39 publications

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Recent Progress in Polyhydroxyalkanoates‐Based Copolymers for Biomedical Applications

The Saline Aquatic Systems As ‘Natural Reservoirs’ For Microorganisms with Current and Potential Applications

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