Engineering <i>Halomonas</i> spp. as A Low‐Cost Production Host for Production of Bio‐surfactant Protein PhaP

Lan, Lu-Hong; Zhao, Han; Chen, Jinchun; Chen, Guoqiang

doi:10.1002/biot.201600459

Cited by 36 publications

(12 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Halophile bacteria Halomonas spp. have been reported to be a convenient platform for economic production of polyhydroxyalkanoates (PHA) bioplastics including poly-3-hydroxybutyrate (PHB), copolymers of 3hydroxybutyrate and 4-hydroxybutyrate or P3HB4HB, copolymers of 3-hydroxybutyrate and 3-hydroxyvalerate or PHBV, as well as PHA granule associated proteins PhaR and PhaP under open and unsterile conditions (Fu et al, 2014;Lan, Zhao, Chen, & Chen, 2016;Simon-Colin, Raguénès, Cozien, & Guezennec, 2008). Halomonas campaniensis was reported to allow a seawater based and 65-day continuous open growth process for PHB production utilizing low-cost carbon source without sterilization (Yue et al, 2014).…”

mentioning

confidence: 99%

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Chen

Qiao

Shuai

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

Halomonas has been developed as a platform for the next generation industrial biotechnology allowing open and nonsterile growth without microbial contamination under a high-salt concentration and alkali pH. To reduce downstream cost associated with continuous centrifugation and salt containing wastewater treatment, Halomonas campaniensis strain LS21 was engineered to become self-flocculating by knocking out an etf operon encoding two subunits of an electron transferring flavoprotein in the predicted electron transfer chain. Self-flocculation could be attributed to the decrease of the surface charge and increase of the cellular hydrophobicity resulted from deleted etf. A wastewaterless fermentation strategy based on the self-flocculating H. campaniensis was developed for growth and the production of poly-3-hydroxybutyrate (PHB) as an example. Most microbial cells flocculated and precipitated to the bottom of the bioreactor within 1 min after stopping the aeration and agitation. The supernatant can be used again without sterilization or inoculation for the growth of the next batch after collecting the precipitated cell mass. The wastewaterless process was conducted for four runs without generating wastewater. PHB accumulation by the self-flocculent strain was enhanced via promoter and ribosome binding site optimizations, the productivities of cell dry weight and PHB were increased from 0.45 and 0.18 g·L −1 ·hr −1 for the batch process compared to 0.82 and 0.33 g·L −1 ·hr −1 for the wastewaterless continuous process, respectively. This has clearly demonstrated the advantages of the wastewaterless process in that it not only reduces wastewater but also increases cell growth and product formation efficiency in a given period of time. K E Y W O R D S continuous process, etf, flocculation, Halomonas campaniensis, next generation industry biotechnology, NGIB, PHB

show abstract

mentioning

confidence: 99%

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Chen

Qiao

Shuai

et al. 2019

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…[121,122] Two PHA synthesis regulatory proteins, PhaR and PhaP, which have amphiphilic property were produced as biosurfactants with good emulsification ability by engineered Halomonas spp. [110,123] Ectoin can also be produced by halophile bacterium Chromohalobacter salexigens at a high-cell-density weighting up to 61 g L −1 , 540 mg ectoine g −1 cells from two continuously operated bioreactors. [124] Other high value-added chemicals like 5-aminolevulinic acid (ALA) and coproduction of ALA and PHB were realized in engineered Halomonas spp.…”

Section: Halomonas Spp As a Platform For Ngibmentioning

confidence: 99%

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Chen

2019

Biotechnology Journal

View full text Add to dashboard Cite

The chemical industry has made a contribution to modern society by providing cost‐competitive products for our daily use. However, it now faces a serious challenge regarding environmental pollutions and greenhouse gas emission. With the rapid development of molecular biology, biochemistry, and synthetic biology, industrial biotechnology has evolved to become more efficient for production of chemicals and materials. However, in contrast to chemical industries, current industrial biotechnology (CIB) is still not competitive for production of chemicals, materials, and biofuels due to their low efficiency and complicated sterilization processes as well as high‐energy consumption. It must be further developed into “next‐generation industrial biotechnology” (NGIB), which is low‐cost mixed substrates based on less freshwater consumption, energy‐saving, and long‐lasting open continuous intelligent processing, overcoming the shortcomings of CIB and transforming the CIB into competitive processes. Contamination‐resistant microorganism as chassis is the key to a successful NGIB, which requires resistance to microbial or phage contaminations, and available tools and methods for metabolic or synthetic biology engineering. This review proposes a list of contamination‐resistant bacteria and takes Halomonas spp. as an example for the production of a variety of products, including polyhydroxyalkanoates under open‐ and continuous‐processing conditions proposed for NGIB.

show abstract

“…Protein production by halophiles has been investigated, including hydrolases amylases, DNases, lipases, proteases and pullulanases,[5a,73] PHA‐associated regulatory protein PhaR, and granule‐associated protein PhaP . The enzymes of halophiles are active at high salt concentrations; they also tolerate more organic solvents and usually more stable at higher temperatures .…”

Section: Protein Production By Halophilesmentioning

confidence: 99%

“…PhaP shows better emulsion properties than that of conventional surfactants such as sodium dodecyl sulfate, sodium oleate, Tween 20, and a liquefied detergent . An effective biosurfactant protein PhaP expression system was developed for H. bluephagenesis TD . The recombinant Halomonas strain induced at 37 °C accumulated 19% PhaP of total soluble proteins reaching 1.86 g L −1 PhaP .…”

Section: Protein Production By Halophilesmentioning

confidence: 99%

Halophiles as Chassis for Bioproduction

2018

Self Cite

View full text Add to dashboard Cite

Industrial biotechnology aims to solve the challenges of petroleum‐based production using microorganisms as catalysts. However, current industrial biotechnology suffers from high energy and fresh water consumption as well as difficult downstream purification. In addition, most industrial microorganisms are not resistant to other microbial contaminations, and this seriously compromises process effectiveness and the economy. The recently proposed “next‐generation industrial biotechnology” employs contamination resistant microorganisms, including alkaliphilic, acidophilic, thermophilic or halophilic bacteria, etc., for bioproduction. The most successful example is the use of halophilic bacteria as chassis for the production of chemicals, proteins, and biopolymers. This review summarizes some of the most recent studies to engineer the halophilic chassis Halomonas spp. for the production of chemicals, proteins, and several biopolyesters such as poly(3‐hydroxybutyrate), copolymers of 3‐hydroxybutyrate and 3‐hydroxyvalerate, and copolymers of 3‐hydroxybutyrate and 4‐hydroxybutyrate. The Halomonas chassis is also successfully engineered to change its morphology from short bars to long fibers and large spheres for convenient gravity separation. This simplifies the bioprocessing.

show abstract

Engineering Halomonas spp. as A Low‐Cost Production Host for Production of Bio‐surfactant Protein PhaP

Cited by 36 publications

References 36 publications

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Engineering self‐flocculating Halomonas campaniensis for wastewaterless open and continuous fermentation

Next‐Generation Industrial Biotechnology‐Transforming the Current Industrial Biotechnology into Competitive Processes

Halophiles as Chassis for Bioproduction

Contact Info

Product

Resources

About