Engineering <i>Yarrowia lipolytica</i> for Enhanced Production of Arbutin

Shang, Yanzhe; Wei, Wenping; Zhang, Ping; Ye, Bang‐Ce

doi:10.1021/acs.jafc.9b07151

Cited by 42 publications

(46 citation statements)

References 47 publications

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“…These results indicate that Y. lipolytica could be a good host for producing shikimate derived molecules, as it has been previously proposed for other compounds derived from aromatic amino acids ( Gu et al., 2020 ), arbutin ( Shang et al., 2020 ), bisdemethoxycurcumin ( Palmer et al., 2020 ), etc. Y. lipolytica , with just the overexpression of the minimal set of enzymes was already able to produce de novo 430 mg/L of resveratrol, while similar modifications in other hosts such as E. coli and S. cerevisiae produced, for example, about 57.77 mg/L ( Zhao et al., 2018 ) and 3.3 mg/L ( Shin et al., 2012 ), respectively.…”

Section: Resultssupporting

confidence: 75%

“…Since resveratrol production requires the condensation of 3 malonyl-CoA molecules, the high availability of this molecule in Y. lipolytica could be advantageous to produce high titers of resveratrol. This organism has been recently engineered to produce different molecules from the shikimate pathway such as 2-phenylethanol, p -coumaric acid, violacein, and deoxyviolacein ( Gu et al., 2020 ), naringenin ( Lv et al., 2020 ; Palmer et al., 2020 ; Wei et al., 2020 ) or arbutin ( Shang et al., 2020 ). Y. lipolytica has also been reported to produce small amount of resveratrol when engineered with such purpose.…”

Section: Introductionmentioning

confidence: 99%

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De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

Szczepańska²,

Yuzbashev³

et al. 2020

Metabolic Engineering Communications

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Resveratrol is a polyphenol with multiple applications in pharma, cosmetics and food. The aim of this study was to construct Yarrowia lipolytica strains able to produce resveratrol. For this purpose, resveratrol-biosynthesis genes from bacteria and plants were expressed in this host. Since resveratrol can be produced either via tyrosine or phenylaniline, both pathways were tested, first with a single copy and then with two copies. The phenylalanine pathway resulted in slightly higher production in glucose media, although when the media was supplemented with amino acids, the best production was found in the strain with two copies of the tyrosine pathway, which reached 0.085 g/L. When glucose was replaced by glycerol, a preferred substrate for bioproduction, the best results, 0.104 g/L, were obtained in a strain combining the expression of the two synthesis pathways. Finally, the best producer strain was tested in bioreactor conditions where a production of 0.43 g/L was reached. This study suggests that Y. lipolytica is a promising host for resveratrol production from glycerol.

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Section: Resultssupporting

confidence: 75%

Section: Introductionmentioning

confidence: 99%

De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

Szczepańska²,

Yuzbashev³

et al. 2020

Metabolic Engineering Communications

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“…In recent years, herbal and medicinal plant extracts such as arbutin have attracted special attention due to multiple biological and pharmacological properties and their unique therapeutic benefits (Shang, Wei, Zhang, & Ye, 2020; Zhu et al, 2018). Kawalier discovered arbutin (in the leaves of Uva ursi ) in 1851.…”

Section: Chemical Characterizations and Therapeutic Effects Of Arbutin In Cosmetic And Pharmaceutical Preparationsmentioning

confidence: 99%

A comprehensive review of the therapeutic potential of α‐arbutin

Saeedi

Khezri

Zakaryaei

et al. 2021

Phytotherapy Research

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Cosmetic dermatology preparations such as bleaching agents are ingredients with skin‐related biological activities for increasing and improving skin beauty. The possibility of controlling skin hyperpigmentation disorders is one of the most important research goals in cosmetic preparations. Recently, cosmetics containing herbal and botanical ingredients have attracted many interests for consumers of cosmetic products because these preparations are found safer than other preparations with synthetic components. However, high‐quality trial studies in larger samples are needed to confirm safety and clinical efficacy of phytotherapeutic agents with high therapeutic index. Arbutin (p‐hydroxyphenyl‐β‐d‐glucopyranoside) is a bioactive hydrophilic polyphenol with two isomers including alpha‐arbutin (4‐hydroxyphenyl‐α‐glucopyranoside) and β‐arbutin (4‐hydroxyphenyl‐β‐glucopyranoside). It is used as a medicinal plant in phytopharmacy. Studies have shown that alpha‐arbutin is 10 times more effective than natural arbutin. A comparison of IC50 values showed that α‐arbutin (with concentration 2.0 mM) has a more potent inhibitory activity on human tyrosinase against natural arbutin (with higher concentration than 30 mM). A review of recent studies showed that arbutin could be beneficial in treatment of various diseases such as hyperpigmentation disorders, types of cancers, central nervous system disorders, osteoporosis, diabetes, etc. This study was designed to describe the therapeutic efficiencies of arbutin.

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“…Yarrowia lipolytica has excellent capability of accumulating acetyl-CoA and malonyl-CoA, and is hence a theoretically preferred host for the production of fatty acids, terpenoids, flavonoids, and other compounds that use acetyl/malonyl-CoA as a precursor (Abdel-Mawgoud et al, 2018). In the past few years, the potential of this host has been greatly explored in the biosynthesis of useful compounds such as lipids and biofuels from various carbon sources (Zhu and Jackson, 2015; Du et al, 2016;Ledesma-Amaro et al, 2016;Lv et al, 2019;Ma et al, 2019;Palmer et al, 2020;Shang et al, 2020); however, xylose-based biosynthesis of natural products has been scarcely investigated despite attempts to understand and improve xylose metabolism in Y. lipolytica (Tsigie et al, 2011;Ryu et al, 2015;Rodriguez et al, 2016;Wei et al, 2020b). On one hand, this is attributed to the lower growth rate and biomass accumulation when cells are cultivated in xylose than in glucose (Ledesma-Amaro et al, 2016); on the other hand, the functional and highly efficient expression of heterologous metabolic pathways relevant to natural product biosynthesis depends on sophisticated tools for genetic manipulation, which are still very limited.…”

Section: Biosynthesis Of Natural Products From Xylose By Yarrowia Lipolyticamentioning

confidence: 99%

Yeast-Based Biosynthesis of Natural Products From Xylose

Zha

Yuwen

Qian

et al. 2021

Front. Bioeng. Biotechnol.

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Xylose is the second most abundant sugar in lignocellulosic hydrolysates. Transformation of xylose into valuable chemicals, such as plant natural products, is a feasible and sustainable route to industrializing biorefinery of biomass materials. Yeast strains, including Saccharomyces cerevisiae, Scheffersomyces stipitis, and Yarrowia lipolytica, display some paramount advantages in expressing heterologous enzymes and pathways from various sources and have been engineered extensively to produce natural products. In this review, we summarize the advances in the development of metabolically engineered yeasts to produce natural products from xylose, including aromatics, terpenoids, and flavonoids. The state-of-the-art metabolic engineering strategies and representative examples are reviewed. Future challenges and perspectives are also discussed on yeast engineering for commercial production of natural products using xylose as feedstocks.

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Engineering Yarrowia lipolytica for Enhanced Production of Arbutin

Cited by 42 publications

References 47 publications

De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

De novo production of resveratrol from glycerol by engineering different metabolic pathways in Yarrowia lipolytica

A comprehensive review of the therapeutic potential of α‐arbutin

Yeast-Based Biosynthesis of Natural Products From Xylose

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