Engineering <i>Yarrowia lipolytica</i> for sustainable production of the chamomile sesquiterpene (−)-α-bisabolol

Ma, Yi-Rong; Li, Wenjuan; Mai, Jie; Wang, Jinpeng; Wei, Yongjun; Ledesma‐Amaro, Rodrigo; Ji, Xiao‐Jun

doi:10.1039/d0gc03180a

Cited by 60 publications

(52 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Y. lipolytica has been previously engineered for the production of various high‐value terpenoids including β‐farnesene, [ 30 ] β‐carotene, [ 16 ] lycopene, [ 26 ] astaxanthin, [ 31 ] betulinic acid, [ 32 ] (‐)‐α‐bisabolol [ 33 ] and so on. [ 34 ] Experimental results such as β‐carotene have reached a titer of 6.5 g L ‐1 (the highest reported so far) after 122 h, [ 24 ] indicate that Y. lipolytica can be engineered using synthetic biology and metabolic engineering to produce terpenoids on an industrial level.…”

Section: Discussionmentioning

confidence: 99%

Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction

Shi

Zhu³

et al. 2021

Biotechnology Journal

View full text Add to dashboard Cite

β‐farnesene is a sesquiterpenoid with various industrial applications which is now commercially produced by a Saccharomyces cerevisiae strain obtained by random mutagenesis and genetic engineering. We rationally designed a genetically defined Yarrowia lipolytica through recovery of L‐leucine biosynthetic route, gene dosage optimization of β‐farnesene synthase and disruption of the competition pathway. The resulting β‐farnesene titer was improved from 8 to 345 mg L‐1. Finally, the strategy for decreasing the lipid accumulation by individually and iteratively knocking out four acyltransferases encoding genes was adopted. The result displayed that β‐farnesene titer in the engineered strain CIBT6304 in which acyltransferases (DGA1 and DGA2) were deleted increased by 45% and reached 539 mg L‐1 (88 mg g‐1 DCW). Using fed‐batch fermentation, CIBT6304 could produce the highest β‐farnesene titer (22.8 g L‐1) among the genetically defined strains. This study will provide the foundation of engineering Y. lipolytica to produce other terpenoids more cost‐efficiently.

show abstract

Section: Discussionmentioning

confidence: 99%

Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction

Shi

Zhu³

et al. 2021

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

“…The yeast Yarrowia lipolytica natively contains the MVA pathway which is thought to be better at supporting the supply of terpene precursors (compared to the MEP pathway which is native in E. coli ), and has a strong acetyl CoA flux, so there is increased interest in using this yeast as a host for terpene synthesis. 386 Y. lipolytica has been engineered to use xylose as a carbon source by CRISPR/Cas9 mediated integration of xylose reductase, xylitol dehydrogenase and xylulose kinase into the genome (Table 1). 387…”

Section: Fragrancementioning

confidence: 99%

The beauty of biocatalysis: sustainable synthesis of ingredients in cosmetics

2022

View full text Add to dashboard Cite

show abstract

“…In order to improve the level, metabolic engineering strategies, including optimizing the mevalonate pathway and the lipid synthesis pathway, were adopted. The (-)-α-bisabolol titer was improved from 8 to 364 mg/L in yeast ( Ma et al, 2021 ). Nevertheless, the production of (-)-α-bisabolol in fungi is still very low ( Table 1 ).…”

Section: Introductionmentioning

confidence: 99%

YALIcloneNHEJ: An Efficient Modular Cloning Toolkit for NHEJ Integration of Multigene Pathway and Terpenoid Production in Yarrowia lipolytica

Yang

Shen

et al. 2022

Front. Bioeng. Biotechnol.

Self Cite

View full text Add to dashboard Cite

Non-homologous end-joining (NHEJ)-mediated random integration in Yarrowia lipolytica has been demonstrated to be an effective strategy for screening hyperproducer strains. However, there was no multigene assembly method applied for NHEJ integration, which made it challenging to construct and integrate metabolic pathways. In this study, a Golden Gate modular cloning system (YALIcloneNHEJ) was established to develop a robust DNA assembly platform in Y. lipolytica. By optimizing key factors, including the amounts of ligase and the reaction cycles, the assembly efficiency of 4, 7, and 10 fragments reached up to 90, 75, and 50%, respectively. This YALIcloneNHEJ system was subsequently applied for the overproduction of the sesquiterpene (-)-α-bisabolol by constructing a biosynthesis route and enhancing the flux in the mevalonate pathway. The resulting strain produced 4.4 g/L (-)-α-bisabolol, the highest titer reported in yeast to date. Our study expands the toolbox of metabolic engineering and is expected to enable a highly efficient production of various terpenoids.

show abstract

Engineering Yarrowia lipolytica for sustainable production of the chamomile sesquiterpene (−)-α-bisabolol

Abstract: The oleaginous yeast Yarrowia lipolytica was metabolically engineered to produce the chamomile sesquiterpene (−)-α-bisabolol in a green and sustainable way.

Cited by 60 publications

References 36 publications

Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction

Engineering the oleaginous yeast Yarrowia lipolytica for β‐farnesene overproduction

The beauty of biocatalysis: sustainable synthesis of ingredients in cosmetics

YALIcloneNHEJ: An Efficient Modular Cloning Toolkit for NHEJ Integration of Multigene Pathway and Terpenoid Production in Yarrowia lipolytica

Contact Info

Product

Resources

About