Engineering lacZ Reporter Gene into an ephA8 Bacterial Artificial Chromosome Using a Highly Efficient Bacterial Recombination System

Kim, Yujin; Song, Eun-Sook; Choi, Sunwoong; Park, Soochul

doi:10.5483/bmbrep.2007.40.5.656

Cited by 10 publications

(21 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The targeting vectors for the EphB2-EphB2 (a full-length mouse Ephb2 cDNA clone) BAC, the EphB2-EphB2-dC (a mouse Ephb2 cDNA clone lacking the coding sequence for amino acids 589-986) BAC, and the EphB2-XIAP (a full-length human XIAP cDNA clone) BAC were constructed as described for EphB2-GFP, except that the GFP reporter and FRT-Kana-FRT cassette were replaced by the indicated cDNA and an IRES-lacZ-FRT-Kana-FRT cassette. The modified recombinant BACs were injected into fertilized C57BL/6 mouse eggs as previously described (Kim et al, 2007b). (005992) (Feldheim et al, 2000), Mapk8 tm1Flv/J (004319) (Dong et al, 1998) and Mapk9 tm1Flv/J (004321) mouse lines were purchased from The Jackson Laboratory.…”

Section: Bac Modification Transgenic and Knockout Micementioning

confidence: 99%

“…We then inserted homologous arms A and B into pGEM11z, a vector containing a GFP reporter with the SV40 polyadenylation site and a FRT-Kana-FRT cassette. The resulting vector was digested with SfiI and inserted into the Ephb2 BAC genomic DNA (RP23-237B18) using a published bacterial homologous recombination method (Kim et al, 2007b). The targeting vectors for the EphB2-EphB2 (a full-length mouse Ephb2 cDNA clone) BAC, the EphB2-EphB2-dC (a mouse Ephb2 cDNA clone lacking the coding sequence for amino acids 589-986) BAC, and the EphB2-XIAP (a full-length human XIAP cDNA clone) BAC were constructed as described for EphB2-GFP, except that the GFP reporter and FRT-Kana-FRT cassette were replaced by the indicated cDNA and an IRES-lacZ-FRT-Kana-FRT cassette.…”

Section: Bac Modification Transgenic and Knockout Micementioning

confidence: 99%

See 1 more Smart Citation

Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling

et al. 2016

View full text Add to dashboard Cite

The development of complex organs such as the eye requires a delicate and coordinated balance of cell division and cell death. Although apoptosis is prevalent in the proximoventral optic cup, the precise role it plays in eye development needs to be investigated further. In this study, we show that reduced apoptosis in the proximoventral optic cup prevents closure of the optic fissure. We also show that expression of ephrin A5 (Efna5) partially overlaps with Eph receptor B2 (Ephb2) expression in the proximoventral optic cup and that binding of EphB2 to ephrin A5 induces a sustained activation of JNK. This prolonged JNK signal promotes apoptosis and prevents cell proliferation. Thus, we propose that the unique cross-subclass interaction of EphB2 with ephrin A5 has evolved to function upstream of JNK signaling for the purpose of maintaining an adequate pool of progenitor cells to ensure proper closure of the optic fissure.

show abstract

Section: Bac Modification Transgenic and Knockout Micementioning

confidence: 99%

Section: Bac Modification Transgenic and Knockout Micementioning

confidence: 99%

Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Homologous BAC recombination was performed as described previously (7). For the enhancer trap assay, a transposable vector carrying a β-globin promoter, LacZ gene containing SV40 poly(A) site, and a kanamycin resistance gene was introduced into each BAC clone according to the method of Spitz et al (8,9).…”

Section: Homologous Bac Recombination and Enhancer Trap Assaymentioning

confidence: 99%

“…BAC DNA for microinjection was confirmed on a gel and diluted with injection buffer as described previously (7). The BAC DNA was injected into 200 pronuclei of fertilized oocytes from C57BL/6 mice as described previously (7,10).…”

Section: Generation Bac Transgenic Micementioning

confidence: 99%

See 1 more Smart Citation

Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer

Kim

Park²

2011

BMB Reports

Self Cite

View full text Add to dashboard Cite

Previous studies suggest that EphA7 plays a critical role in neural tube closure or cortical progenitor apoptosis. In this report, enhancer trap assay was used to modify various EphA7 BAC clones and screen a large genomic region spanning 570 kb downstream of the EphA7 gene. We found that the dorsal midline-specific EphA7 enhancer resides on the 457D20 EphA7 BAC clone and is localized to a 35 kb genomic region in between +345.7 kb to +379.

show abstract

Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development

Kim

Park

Noh

et al. 2013

Developmental Neurobiology

View full text Add to dashboard Cite

EphAs and ephrin-As are expressed in multiple regions of the developing brain and have been implicated in regulating brain size. Here, we report the identification of a novel mechanism in which reverse signaling through ephrin-As controls neural epithelial cell number in the developing brain. Ectopic expression of EphA8-Fc in transgenic embryos induced apoptosis of neural epithelial cells, which was accompanied by a dramatic decrease in brain size. The number of ephrin-A5-expressing cells was significantly reduced in the brain region where EphA8-Fc was ectopically expressed. Furthermore, in vitro culture of the dissociated neuroepithelial cells revealed that EphA8-Fc enhanced apoptotic cell death of the ephrinA5-expressing cells in a caspase-dependent manner. Thus, our results suggest that reverse signaling through ephrin-As is biochemically linked with caspase-dependent proapoptotic signaling during early brain development.

show abstract

Engineering lacZ Reporter Gene into an ephA8 Bacterial Artificial Chromosome Using a Highly Efficient Bacterial Recombination System

Cited by 10 publications

References 30 publications

Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling

Proper closure of the optic fissure requires ephrin A5-EphB2-JNK signaling

Identification of EphA7 BAC clone containing a long-range dorsal midline-specific enhancer

Expression of EphA8‐Fc in transgenic mouse embryos induces apoptosis of neural epithelial cells during brain development

Contact Info

Product

Resources

About