Engineering microbial membranes to increase stress tolerance of industrial strains

Qi, Yanli; Liu, Hui; Chen, Xiulai; Li, Liming

doi:10.1016/j.ymben.2018.12.010

Cited by 113 publications

(87 citation statements)

References 126 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Resistance to acetic acid has also been reported through the overexpression of WHI2 (a cytoplasmatic globular scaffold protein), in conjunction with PSR1 (plasma membrane phosphatase) . Engineering microbial membranes towards maintaining or enhancing the plasma membrane properties can therefore improve the productivity of industrial biocatalysts …”

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Brandt

Jansen

Görgens

et al. 2019

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Lignocellulose-derived biofuels present an attractive carbon-neutral alternative to fossil fuels amidst growing global energy and climate concerns. Bioethanol in particular, has been shown to be a viable additive to and / or replacement for petroleum in countries such as Brazil. The bioconversion of lignocellulose biomass to bioethanol is a developing technology with the yeast Saccharomyces cerevisiae playing a pivotal role in the fermentation-based processes. This yeast however, is challenged to ferment under harsh conditions, specifically, during exposure to lignocellulose-derived microbial inhibitors that are formed during pretreatment processes. This detrimentally affects biocatalyst performance, ultimately decreasing ethanol productivity and yield. To this end, S. cerevisiae strains are in development to increase the yeast microbial inhibitor resistance towards cost-effective cellulosic bioethanol production. This review discusses the current status of inhibitor resistance in S. cerevisiae strains and perspectives on the future of multi-tolerant phenotypes with a specific focus on existing and emerging strain development strategies designed to improve resistance phenotypes.

show abstract

Section: Expanding S Cerevisiae Inhibitor Resistancementioning

confidence: 99%

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Brandt

Jansen

Görgens

et al. 2019

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

“…Chemical stress can have inhibitory effects on the host organism, limiting the achievable titers and thereby the economic feasibility of the production process. These issues can be overcome by engineering a production strain that is tolerant to higher product titers, but this is rarely possible through rational engineering due to lack of knowledge about the molecular mechanisms of chemical toxicity or tolerance 3 . This requires either choosing a more robust, but potentially otherwise difficult to engineer production organism, or alternatively using non-rational approaches to engineer tolerance.…”

Section: Introductionmentioning

confidence: 99%

Adaptive laboratory evolution reveals general and specific chemical tolerance mechanisms and enhances biochemical production

Lennen

Jensen

Mohammed

et al. 2019

Preprint

View full text Add to dashboard Cite

Tolerance to high product concentrations is a major barrier to achieving economically viable processes for bio-based chemical production. Chemical tolerance mechanisms are often unknown, thus their rational design is not achievable. To reveal unknown tolerance mechanisms we used an automated platform to evolve Escherichia coli to grow in previously toxic concentrations of 11 chemicals that have applications as polymer precursors, chemical intermediates, or biofuels. Re-sequencing of isolates from 88 independently evolved 2 populations, reconstruction of mutations, and cross-compound tolerance profiling was employed to uncover general and specific tolerance mechanisms. We found that: 1) the broad tolerance of strains towards chemicals varied significantly depending on the chemical stress condition under which the strain was evolved; 2) the strains that acquired high levels of NaCl tolerance also became broadly tolerant to most chemicals; 3) genetic tolerance mechanisms included alterations in regulatory, cell wall, transcriptional and translational functions, as well as more chemical-specific mechanisms related to transport and metabolism; 4) using pre-tolerized starting strains can significantly enhance subsequent production of chemicals when a production pathway is inserted; and 5) only a subset of the evolved isolates showed improved production indicating that this approach is especially useful when a large number of independently evolved isolates are screened for production.We provide a comprehensive genotype-phenotype map based on identified mutations and growth phenotypes for 224 chemical tolerant strains.

show abstract

“…The membrane is a natural barrier separating extracellular conditions from intracellular components (4). Therefore, improving membrane function is a potential strategy to enhance the growth performance of industrial strains under harsh industrial conditions (5).…”

Section: Introductionmentioning

confidence: 99%

“…Microbial membranes are primarily composed of a mix of proteins and lipids, and manipulating these components may regulate the physiological function and growth of industrial strains (5). Cell membrane proteins have a number of different functions, but membrane protein engineering is focused on transporters as follows (3): (i) Engineering influx transporters: Expressing influx transporters is a strategy used to increase carbon flux for product synthesis (6).…”

Section: Introductionmentioning

confidence: 99%

Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiae

Zhu

Yin

Luo

et al. 2019

Preprint

Self Cite

View full text Add to dashboard Cite

20In order to enhance the growth performance of S. cerevisiae under harsh environmental conditions, 21 mutant XCG001, which tolerates up to 1.5M NaCl, was isolated via adaptive laboratory evolution 22 IMPORTANCE 33This study demonstrated a novel strategy for manipulation membrane complex sphingolipids to 34 enhance S. cerevisiae tolerance to osmotic stress. Osmotic tolerance was related to sphingolipid 35 acyl chain elongase, Elo2, via transcriptome analysis of the wild-type strain and an osmotic tolerant 36 strain generated from ALE. Overexpression of ELO2 increased complex sphingolipid with longer 37 3 acyl chain, thus improved membrane integrity and osmotic tolerance. 38 KERWORDS: Adaptive laboratory evolution; osmotic tolerance; membrane engineering; 39 complex sphingolipid; membrane integrity 40

show abstract

Engineering microbial membranes to increase stress tolerance of industrial strains

Cited by 113 publications

References 126 publications

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Overcoming lignocellulose‐derived microbial inhibitors: advancing the Saccharomyces cerevisiae resistance toolbox

Adaptive laboratory evolution reveals general and specific chemical tolerance mechanisms and enhances biochemical production

Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiae

Contact Info

Product

Resources

About