Engineering Nanoscale Stem Cell Niche: Direct Stem Cell Behavior at Cell–Matrix Interface

Zhang, Yan; Gordon, Andrew H.; Qian, Weiyi; Chen, Weiqiang

doi:10.1002/adhm.201500351

Cited by 38 publications

(35 citation statements)

References 211 publications

(328 reference statements)

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“…This, in turn, may enable the generation of diversity in tissue/organotypic cultures. For a detailed discussion on the scientific details of tools and approaches to control cell behavior and function, please refer to recent reviews (Kim & Kino‐oka, ; Kim & Kino‐oka, ; Zhang, Gordon, Qian, & Chen, ). Design of the culturing environment: the development of approaches for controlling the cellular microenvironment of pluripotent and multipotent cells represents a fundamental aspect of stem cell bioprocessing. Indeed, as in the body, cells are extremely sensitive to environmental variables such as mechanical regulation (e.g., hydrostatic pressure, fluid flow, and stress) and physiologically relevant environmental conditions (e.g., temperature, pH, and CO 2 ; McKee & Chaudhry, ).…”

Section: Challenges In Designing a Blueprint For The Success Of Stem mentioning

confidence: 99%

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Designing a blueprint for next‐generation stem cell bioprocessing development

Kim

Kino‐oka

2019

Biotech & Bioengineering

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The creation of a blueprint for stem cell bioprocess development that it is easily readable and shareable among those involved in the construction of the bioprocess is a necessary step toward full-fledged bioprocess integration. The blueprint provides the culturing tools and methodologies, designed to highlight knowledge gaps within biological sciences and bioengineering. This review highlights a blueprint for stem cell bioprocessing development using a landscape architecture approach that can aid the development of culture technologies and tools that satisfy the demands for stem cell-derived products for use in clinical and industrial applications. This work is intended to provide insights to cell biologists, geneticists, bioengineers, and clinicians seeking knowledge outside of their field of expertise and fosters a leap from a reductionist approach to one, that is, globally integrated in stem cell bioprocessing. K E Y W O R D S bioprocessing blueprint, culture technologies and tools, stem cell, Waddington's epigenetic landscape

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Section: Challenges In Designing a Blueprint For The Success Of Stem mentioning

confidence: 99%

Section: Design Elements and Principlesmentioning

confidence: 99%

Designing a blueprint for next‐generation stem cell bioprocessing development

Kim

Kino‐oka

2019

Biotech & Bioengineering

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“…[76][77][78][79][80][81][82] There are excellent reviews on the effect of nanotopography on (stem) cells. [81,83] …”

Section: Geometrymentioning

confidence: 99%

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

Cipitria

Salmerón-Sánchez

2017

Adv Healthcare Materials

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Engineering cellular microenvironments involves biochemical factors, the extracellular matrix (ECM) and the interaction with neighbouring cells. This progress report provides a critical overview of key studies that incorporate growth factor (GF) signalling and mechanotransduction into the design of advanced microenvironments. Materials systems have been developed for surface‐bound presentation of GFs, either covalently tethered or sequestered through physico‐chemical affinity to the matrix, as an alternative to soluble GFs. Furthermore, some materials contain both GF and integrin binding regions and thereby enable synergistic signalling between the two. Mechanotransduction refers to the ability of the cells to sense physical properties of the ECM and to transduce them into biochemical signals. Various aspects of the physics of the ECM, i.e. stiffness, geometry and ligand spacing, as well as time‐dependent properties, such as matrix stiffening, degradability, viscoelasticity, surface mobility as well as spatial patterns and gradients of physical cues are discussed. To conclude, various examples illustrate the potential for cooperative signalling of growth factors and the physical properties of the microenvironment for potential applications in regenerative medicine, cancer research and drug testing.

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“…[16][17][18] Nanotopographic cues affect stem cell adhesion, migration, proliferation, differentiation, and apoptosis by adjusting their structures, surface stiffness, and porosity. 19 Tendons are aligned structures, and decreased tendon function caused by tendinopathy and tendon injury is associated with tendon misalignment. 20 Therefore, alignment of TDSCs is important in tendon regeneration and healing; well-aligned fibers commit stem cells to teno-lineage differentiation.…”

Section: Introductionmentioning

confidence: 99%

Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions

Kim¹,

Tatman²,

Song³

et al. 2018

IJN

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BackgroundTendon-derived stem cells (TDSCs) are key factors associated with regeneration and healing in tendinopathy. The aim of this study was to investigate the effects of mechanical stiffness and topographic signals on the differentiation of TDSCs depending on age and pathological conditions.Materials and methodsWe compared TDSCs extracted from normal tendon tissues with TDSCs from tendinopathic Achilles tendon tissues of Sprague Dawley rats in vitro and TDSCs cultured on nanotopographic cues and substrate stiffness to determine how to control the TDSCs. The tendinopathy model was created using a chemical induction method, and the tendon injury model was created via an injury-and-overuse method. Norland Optical Adhesive 86 (NOA86) substrate with 2.48 GPa stiffness with and without 800 nm-wide nanogrooves and a polyurethane substrate with 800 nm-wide nanogrooves were used.ResultsTDSCs from 5-week-old normal tendon showed high expression of type III collagen on the flat NOA86 substrate. In the 15-week normal tendon model, expression of type III collagen was high in TDSCs cultured on the 800 nm NOA86 substrates. However, in the 15-week tendon injury model, expression of type III collagen was similar irrespective of nanotopographic cues or substrate stiffness. The expression of type I collagen was also independent of nanotopographic cues and substrate stiffness in the 15-week normal and tendon injury models. Gene expression of scleraxis was increased in TDSCs cultured on the flat NOA86 substrate in the 5-week normal tendon model (P=0.001). In the 15-week normal tendon model, scleraxis was highly expressed in TDSCs cultured on the 800 nm and flat NOA86 substrate (P=0.043). However, this gene expression was not significantly different between the substrates in the 5-week tendinopathy and 15-week tendon injury models.ConclusionDevelopment and maturation of tendon are enhanced when TDSCs from normal tendons were cultured on stiff surface, but not when the TDSCs came from pathologic models. Therapeutic applications of TDSCs need to be flexible based on tendon age and tendinopathy.

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Engineering Nanoscale Stem Cell Niche: Direct Stem Cell Behavior at Cell–Matrix Interface

Cited by 38 publications

References 211 publications

Designing a blueprint for next‐generation stem cell bioprocessing development

Designing a blueprint for next‐generation stem cell bioprocessing development

Mechanotransduction and Growth Factor Signalling to Engineer Cellular Microenvironments

Nanotopographic cues and stiffness control of tendon-derived stem cells from diverse conditions

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