Engineering of a bi-enzymatic reaction for efficient production of the ascorbic acid precursor 2-keto-l-gulonic acid

Kaswurm, Vanja; Hecke, Wouter Van; Kulbe, Klaus D.; Ludwig, Roland

doi:10.1016/j.bej.2013.07.010

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…To improve the fermentation performance, synergic microorganisms such as Pseudomonas striata with G. oxydans spp. (later characterized and identified as Ketogulonicigenium vulgare) or enzymes [43,44] that generate vitamin C via the direct oxidation of L-sorbosone (an intermediate of the bio-oxidation of D-sorbitol to 2KGA) were investigated for vitamin C production. These open the possibility for a direct route production of vitamin C, eliminating the chemical rearrangement necessity of 2KGA [9,28].…”

Section: Two-step Fermentation Processmentioning

confidence: 99%

Strain Development, Substrate Utilization, and Downstream Purification of Vitamin C

Tucaliuc¹,

Cîșlaru²,

Kloetzer³

et al. 2022

Processes

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Vitamin C, C6H8O6, is a water-soluble vitamin that is widespread in nature. It is an essential nutrient involved in many biological processes in the living organisms: it enhances collagen biosynthesis, ensures the optimal functioning of enzymes and the immune system, has a major role in lipid and iron metabolism, and it enhances the biosynthesis of l-carnitine. Due to its antioxidant activity, vitamin C can neutralize the tissue-damaging effects of free radicals. Vitamin C is being related to the prevention of cancer and cardiovascular diseases. This review includes current information on the biosynthesis of ascorbic acid, as new methods are now challenging the traditional Reichstein process for vitamin C’s industrial-scale production. Different strains were analyzed in correlation with their ability to synthesize ascorbic acid, and several separation techniques were investigated for a more effective production of vitamin C.

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Section: Two-step Fermentation Processmentioning

confidence: 99%

Strain Development, Substrate Utilization, and Downstream Purification of Vitamin C

Tucaliuc¹,

Cîșlaru²,

Kloetzer³

et al. 2022

Processes

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show abstract

“…However, by implementing a regeneration system, the cofactor can be reused, and addition of expensive cofactors can thus be kept at a minimum, ensuring higher yield at reduced costs. For instance, adding glucose and glucose dehydrogenase (GDH) for the regeneration of NADPH by reduction of NADP+ represents a strategy frequently employed in the pharmaceutical and chemical industries [125].…”

Section: Reduction To Furan Alcoholsmentioning

confidence: 99%

Novel Routes in Transformation of Lignocellulosic Biomass to Furan Platform Chemicals: From Pretreatment to Enzyme Catalysis

2020

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The constant depletion of fossil fuels along with the increasing need for novel materials, necessitate the development of alternative routes for polymer synthesis. Lignocellulosic biomass, the most abundant carbon source on the planet, can serve as a renewable starting material for the design of environmentally-friendly processes for the synthesis of polyesters, polyamides and other polymers with significant value. The present review provides an overview of the main processes that have been reported throughout the literature for the production of bio-based monomers from lignocellulose, focusing on physicochemical procedures and biocatalysis. An extensive description of all different stages for the production of furans is presented, starting from physicochemical pretreatment of biomass and biocatalytic decomposition to monomeric sugars, coupled with isomerization by enzymes prior to chemical dehydration by acid Lewis catalysts. A summary of all biotransformations of furans carried out by enzymes is also described, focusing on galactose, glyoxal and aryl-alcohol oxidases, monooxygenases and transaminases for the production of oxidized derivatives and amines. The increased interest in these products in polymer chemistry can lead to a redirection of biomass valorization from second generation biofuels to chemical synthesis, by creating novel pathways to produce bio-based polymers.

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“…The full rate equation of Bacillus sp. GDH, which is often applied for the regeneration of both NADPH and NADH, was recently elucidated [19]. Furthermore, modeling provides guidance in converting batch to continuous conversions as recently demonstrated for lactobionic acid production [20].…”

Section: Introductionmentioning

confidence: 99%

Engineering an enzymatic regeneration system for NAD(P)H oxidation

Pham

Hollmann

Kracher

et al. 2015

Journal of Molecular Catalysis B: Enzymatic

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a b s t r a c tA recently proposed coenzyme regeneration system employing laccase and a number of various redox mediators for the oxidation of NAD(P)H was studied in detail by kinetic characterization of individual reaction steps. Reaction engineering by modeling was used to optimize the employed enzyme, coenzyme as well as redox mediator concentrations. Glucose dehydrogenase from Bacillus sp. served as a convenient model of synthetic enzymes that depend either on NAD + or NADP + . The suitability of laccase from Trametes pubescens in combination with acetosyringone or syringaldazine as redox mediator was tested for the regeneration (oxidation) of both coenzymes. In a first step, pH profiles and catalytic constants of laccase for the redox mediators were determined. Then, second-order rate constants for the oxidation of NAD(P)H by the redox mediators were measured. In a third step, the rate equation for the entire enzymatic process was derived and used to build a MATLAB model. After verifying the agreement of predicted vs. experimental data, the model was used to calculate different scenarios employing varying concentrations of regeneration system components. The modeled processes were experimentally tested and the results compared to the predictions. It was found that the regeneration of NADH to its oxidized form was performed very efficiently, but that an excess of laccase activity leads to a high concentration of the oxidized form of the redox mediator -a phenoxy radical -which initiates coupling (dimerization or polymerization) and enzyme deactivation.

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Engineering of a bi-enzymatic reaction for efficient production of the ascorbic acid precursor 2-keto-l-gulonic acid

Cited by 8 publications

References 26 publications

Strain Development, Substrate Utilization, and Downstream Purification of Vitamin C

Strain Development, Substrate Utilization, and Downstream Purification of Vitamin C

Novel Routes in Transformation of Lignocellulosic Biomass to Furan Platform Chemicals: From Pretreatment to Enzyme Catalysis

Engineering an enzymatic regeneration system for NAD(P)H oxidation

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