Engineering of a polymer layered bio-hybrid heart valve scaffold

Jahnavi, S.; Kumary, T. V.; Bhuvaneshwar, G.S.; Natarajan, T. S.; Verma, Rama Shanker

doi:10.1016/j.msec.2015.03.009

Cited by 33 publications

(30 citation statements)

References 38 publications

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“…By integrating the ECM directly into the polymer fibers, as we have done in this study using electrospinning, we are able to create a highly tailored and physically repeatable structure that both includes the biochemical cues found in native ECM, and has the mechanical strength of the electrospun polymer. Moreover, as found in this study and previous, there are mechanical and biochemical benefits to combining ECM with polymers (Jahnavi et al, ; Wu et al, ).…”

Section: Discussionsupporting

confidence: 83%

“…In this study, we have combined the two in an attempt to harness the beneficial characteristics from each to create a bioscaffold more suited for vascular tissue engineering. Recently, work has focused on combining the two to take advantage of these characteristics, in a similar vein to this study (Gao et al, ; Hong et al, ; Jahnavi et al, ; Jiang et al, ; Wu et al, ). For example, coating vascular ECM with polymers has been implemented before, with the aim of improving biochemical and mechanical performance (Jahnavi et al, ; Wu et al, ).…”

Section: Discussionmentioning

confidence: 79%

“…Other methods of manufacturing scaffolds/constructs using vascular ECM and polymers have ranged from repopulating aortic valves to vascular grafts in the cardiovascular realm (Schoen, Avrahami, Baruch, et al, 2017). Interestingly, studies focusing on vascular ECM have shown improvements in native cell repopulation and mechanical properties when combining the ECM with a polymer (Hong et al, 2008;Jahnavi, Kumary, Bhuvaneshwar, Natarajan, & Verma, 2015;Jiang et al, 2015;Wu et al, 2007). Furthermore, electrospinning was successfully utilized to generate these hybrid structures that showed increased mechanical strength and improved cellular repopulation (Hong et al, 2008;Jahnavi et al, 2015;Wu et al, 2007).…”

mentioning

confidence: 99%

“…Interestingly, studies focusing on vascular ECM have shown improvements in native cell repopulation and mechanical properties when combining the ECM with a polymer (Hong et al, 2008;Jahnavi, Kumary, Bhuvaneshwar, Natarajan, & Verma, 2015;Jiang et al, 2015;Wu et al, 2007). Furthermore, electrospinning was successfully utilized to generate these hybrid structures that showed increased mechanical strength and improved cellular repopulation (Hong et al, 2008;Jahnavi et al, 2015;Wu et al, 2007). These previous studies combining vascular ECM and polymers to create hybrid scaffolds show promising results, with improvements in cellular performance noted in most cases.…”

mentioning

confidence: 99%

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Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering

Reid

Callanan

2019

J Biomed Mater Res

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The aim when designing a scaffold is to provide a supportive microenvironment for the native cells, which is generally achieved by structurally and biochemically imitating the native tissue. Decellularized extracellular matrix (ECM) possesses the mechanical and biochemical cues designed to promote native cell survival. However, when decellularized and reprocessed, the ECM loses its cell supporting mechanical integrity and architecture. Herein, we propose dissolving the ECM into a polymer/solvent solution and electrospinning it into a fibrous sheet, thus harnessing the biochemical cues from the ECM and the mechanical integrity of the polymer. Bovine aorta and myocardium were selected as ECM sources. Decellularization was achieved using sodium dodecyl sulfate (SDS), and the ECM was combined with polycaprolactone and hexafluoro‐2‐propanol for electrospinning. The scaffolds were seeded with human umbilical vein endothelial cells (HUVECs). The study found that the inclusion of aorta ECM increased the scaffold's wettability and subsequently lead to increased HUVEC adherence and proliferation. Interestingly, the inclusion of myocardium ECM had no effect on wettability or cell viability. Furthermore, gene expression and mechanical changes were noted with the addition of ECM. The results from this study show the vast potential of electrospun ECM/polymer bioscaffolds and their use in tissue engineering.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 79%

mentioning

confidence: 99%

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confidence: 99%

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Hybrid cardiovascular sourced extracellular matrix scaffolds as possible platforms for vascular tissue engineering

Reid

Callanan

2019

J Biomed Mater Res

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“…However, pure polymeric nanofibers are suitable mainly for soft tissue engineering, such as reconstruction and regeneration of blood vessels [13,22,23,31,32], myocardium [33,34], heart valves [35,36], skeletal muscle [37,38], skin [15, [39][40][41], tendon and ligament [42,43], intestine [44,45], tissues of the respiratory system, such as trachea and bronchi [46,47], components of urinary tract, such as bladder [48] and urethra [49], visceral organs, such as liver [50,51] or pancreas (pancreatic islets [52,53]), central nervous system, such as brain [6,54,55], spinal cord [56,57], optic system, such as optical nerve [58] and retina [59], and peripheral nervous system [17,60]. Nanofibrous scaffolds can be associated with another advanced technique in recent tissue engineering-controlled delivery of various types of stem cells, such as bone marrow mesenchymal stem cells [51,[61][62][63], adipose tissue-derived stem cells [64,65], neural tissue-derived stem cells [57], and induced pluripotent stem cells …”

Section: Introductionmentioning

confidence: 99%

Nanofibrous Scaffolds as Promising Cell Carriers for Tissue Engineering

Bačáková¹,

Bačáková²,

Pajorová³

et al. 2016

Nanofiber Research - Reaching New Heights

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Nanofibers are promising cell carriers for tissue engineering of a variety of tissues and organs in the human organism. They have been experimentally used for reconstruction of tissues of cardiovascular, respiratory, digestive, urinary, nervous and musculoskeletal systems. Nanofibers are also promising for drug and gene delivery, construction of biosensors and biostimulators, and wound dressings. Nanofibers can be created from a wide range of natural polymers or synthetic biostable and biodegradable polymers. For hard tissue engineering, polymeric nanofibers can be reinforced with various ceramic, metal-based or carbon-based nanoparticles, or created directly from hard materials. The nanofibrous scaffolds can be loaded with various bioactive molecules, such as growth, differentiation and angiogenic factors, or funcionalized with ligands for the cell adhesion receptors. This review also includes our experience in skin tissue engineering using nanofibers fabricated from polycaprolactone and its copolymer with polylactide, cellulose acetate, and particularly from polylactide nanofibers modified by plasma activation and fibrin coating. In addition, we studied the interaction of human bone-derived cells with nanofibrous scaffolds loaded with hydroxyapatite or diamond nanoparticles. We also created novel nanofibers based on diamond deposition on a SiO 2 template, and tested their effects on the adhesion, viability and growth of human vascular endothelial cells.

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