Engineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids

Hadiati, Atika; Krahn, Irene; Lindner, Steffen N.; Wendisch, Volker F.

doi:10.1186/s40643-014-0025-5

Cited by 21 publications

(9 citation statements)

References 58 publications

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“…Notable improvements in glycerol consumption were observed upon the addition of glucuronic acid (45% glycerol consumption and Y SA = 0.42) and galacturonic acid (48% glycerol consumption and Y SA = 0.33); these improvements are similar to those obtained with the two aforementioned acids (fumaric and malic). This comparison, however, indicates the superiority of fumaric and malic acid addition with regard to the specific production of succinic acid (Y SA ), which is likely attributable to the more oxidized character of these compounds compared to glucose (Clomburg and Gonzalez, 2013), which can partially compensate for the highly reduced glycerol compounds in nature (Hadiati et al, 2014). According to the auxiliary concept of Babel (2009), the simultaneous consumption of physiologically similar substrates can increase the yield of a target product.…”

Section: Resultsmentioning

confidence: 99%

Enterobacter sp. LU1 as a novel succinic acid producer – co‐utilization of glycerol and lactose

Podleśny

Jarocki

Wyrostek

et al. 2016

Microbial Biotechnology

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SummarySuccinic acid is an important C4‐building chemical platform for many applications. A novel succinic acid‐producing bacterial strain was isolated from goat rumen. Phylogenetic analysis based on the 16S rRNA sequence and physiological analysis indicated that the strain belongs to the genus Enterobacter. This is the first report of a wild bacterial strain from the genus Enterobacter that is capable of efficient succinic acid production. Co‐fermentation of glycerol and lactose significantly improved glycerol utilization under anaerobic conditions, debottlenecking the utilization pathway of this valuable biodiesel waste product. Succinic acid production reached 35 g l−1 when Enterobacter sp. LU1 was cultured in medium containing 50 g l−1 of glycerol and 25 g l−1 of lactose as carbon sources.

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Section: Resultsmentioning

confidence: 99%

Enterobacter sp. LU1 as a novel succinic acid producer – co‐utilization of glycerol and lactose

Podleśny

Jarocki

Wyrostek

et al. 2016

Microbial Biotechnology

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“…Finally, by the action of 2‐keto‐3‐deoxygluconate‐6‐phosphate aldolase ( eda ), 2‐dehydro‐3‐deoxy‐D‐gluconate 6‐phosphate is further degraded into pyruvate and D‐glyceraldehyde 3‐phosphate. By the introduction of these genes, the recombinant strains C. glutamicum DM1933, C. glutamicum Δ crtYEb and C. glutamicum ORN1 were able to produce 0.04–0.07 g/g of l ‐lysine, 0.08–0.09 g/g of lycopene and 0.001–0.01 g/g of l ‐ornithine from hexuronic acid …”

Section: Utilization Of Biomass For the Production Of Chemicals And Pmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…C. glutamicum utilizes a large variety of sugars and organic acids as sources of carbon and energy (6,7) and additionally has been genetically engineered for the utilization of alternative feedstocks such as starch, glycerol, xylose, glucuronic acid, and N-acetylglucosamine (8)(9)(10)(11)(12). In contrast to many other bacterial species, C. glutamicum prefers to use multiple carbon sources simultaneously.…”

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confidence: 99%

Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR

et al. 2016

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Corynebacterium glutamicum metabolizes sialic acid (Neu5Ac) to fructose-6-phosphate (fructose-6P) via the consecutive activity of the sialic acid importer SiaEFGI, N-acetylneuraminic acid lyase (NanA), N-acetylmannosamine kinase (NanK), N-acetylmannosamine-6P epimerase (NanE), N-acetylglucosamine-6P deacetylase (NagA), and glucosamine-6P deaminase (NagB). Within the cluster of the three operons nagAB, nanAKE, and siaEFGI for Neu5Ac utilization a fourth operon is present, which comprises cg2936, encoding a GntR-type transcriptional regulator, here named NanR. Microarray studies and reporter gene assays showed that nagAB, nanAKE, siaEFGI, and nanR are repressed in wild-type (WT) C. glutamicum but highly induced in a ⌬nanR C. glutamicum mutant. Purified NanR was found to specifically bind to the nucleotide motifs A [AC]G[CT][AC]TGATGT C[AT][TG]ATGT[AC]TA located within the nagA-nanA and nanR-sialA intergenic regions. Binding of NanR to promoter regions was abolished in the presence of the Neu5Ac metabolism intermediates GlcNAc-6P and N-acetylmannosamine-6-phosphate (ManNAc-6P). We observed consecutive utilization of glucose and Neu5Ac as well as fructose and Neu5Ac by WT C. glutamicum, whereas the deletion mutant C. glutamicum ⌬nanR simultaneously consumed these sugars. Increased reporter gene activities for nagAB, nanAKE, and nanR were observed in cultivations of WT C. glutamicum with Neu5Ac as the sole substrate compared to cultivations when fructose was present. Taken together, our findings show that Neu5Ac metabolism in C. glutamicum is subject to catabolite repression, which involves control by the repressor NanR. IMPORTANCENeu5Ac utilization is currently regarded as a common trait of both pathogenic and commensal bacteria. Interestingly, the nonpathogenic soil bacterium C. glutamicum efficiently utilizes Neu5Ac as a substrate for growth. Expression of genes for Neu5Ac utilization in C. glutamicum is here shown to depend on the transcriptional regulator NanR, which is the first GntR-type regulator of Neu5Ac metabolism not to use Neu5Ac as effector but relies instead on the inducers GlcNAc-6P and ManNAc-6P. The identification of conserved NanR-binding sites in intergenic regions within the operons for Neu5Ac utilization in pathogenic Corynebacterium species indicates that the mechanism for the control of Neu5Ac catabolism in C. glutamicum by NanR as described in this work is probably conserved within this genus. The Gram-positive Corynebacterium glutamicum is mostly known for its application in the industrial production of amino acids, mainly L-glutamate and L-lysine (1, 2), and has become a versatile cell factory for the production of various commodity products (3-5). C. glutamicum utilizes a large variety of sugars and organic acids as sources of carbon and energy (6, 7) and additionally has been genetically engineered for the utilization of alternative feedstocks such as starch, glycerol, xylose, glucuronic acid, and N-acetylglucosamine (8-12). In contrast to many other bacterial species, C. glutamic...

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Engineering of Corynebacterium glutamicum for growth and production of L-ornithine, L-lysine, and lycopene from hexuronic acids

Cited by 21 publications

References 58 publications

Enterobacter sp. LU1 as a novel succinic acid producer – co‐utilization of glycerol and lactose

Enterobacter sp. LU1 as a novel succinic acid producer – co‐utilization of glycerol and lactose

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Transcription of Sialic Acid Catabolism Genes in Corynebacterium glutamicum Is Subject to Catabolite Repression and Control by the Transcriptional Repressor NanR

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