Engineering of formate dehydrogenase for improving conversion potential of carbon dioxide to formate

Abstract

Formate dehydrogenase (FDH) is a D-2-hydroxy acid dehydrogenase, which can reversibly reduce CO 2 to formate and thus act as non-photosynthetic CO 2 reductase. In order to increase catalytic e ciency of formate dehydrogenase for CO 2 reduction, the formate dehydrogenase CbFDH M2 was used as the parent to conduct single-site saturation and iterative combinatorial mutagenesis, and two mutants V328I/F285W and V354G/F285W were obtained of which reduction activity was about two times more than the CbFDH M2 and had greater potential in CO 2 reduction. The optimal temperature for V328I/F285W and V354G/F285W was 55℃, and the optimal pH was 9.0. Then the molecular basis for its improvement of biochemical characteristics were preliminarily elucidated by computer-aided methods. All of these results further established a solid foundation for molecular modi cation of formate dehydrogenase and CO 2 reduction.

Engineered Escherichia coli Whole Cell-Mediated Electro-Biocatalysis for Carbon Dioxide to Formic Acid Conversion

“…1.2.1.2) acts as a biocatalyst to catalyze the oxidation of formic acid to CO 2 and transfers electrons to the oxidized coenzyme (S ox ) or reversely reduces CO 2 to formic acid and transfers electrons to the reduced coenzyme (S red ), as shown in eq HCO 2 normalH + S ox ⇌ FDH CO 2 + S red + 2 H + Depending on the presence or absence of metal cofactors, FDH can be classified into simple NAD + -dependent and NAD + /metal-containing types. NAD + /metal-containing FDHs have a metal cluster in their activity center, which is not the case for simple NAD + -dependent FDHs . NAD + /metal-containing FDHs can react not only with natural cofactors (NAD + /NADH) for formic acid oxidation/CO 2 reduction but also with artificial electron acceptors/donors .…”

Current status and emerging frontiers in enzyme engineering: An industrial perspective

“…Sequence-based and structure-based methods are typically used to obtain information regarding the structure and function of proteins [ 62 ]. The sequence-based method involves aligning the target protein's sequence with a well-characterized homologous protein sequence [ 63 ].…”