Engineering of glycerol utilization in Pseudomonas chlororaphis GP72 for enhancing phenazine-1-carboxylic acid production

Song, Chao; Yue, Sheng‐Jie; Liu, Wenhui; Zheng, Yifan; Zhang, Chenghao; Feng, Tong‐Tong; Hu, Hongbo; Wang, Wei; Zhang, Xuehong

doi:10.1007/s11274-020-02824-3

Cited by 21 publications

(26 citation statements)

References 30 publications

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“…Another commercial product is shenqinmycin, based on PCA. It has been in use since 2011 to control fungal diseases [ 160 ]. Metabolites from Streptomyces spp.…”

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

“…To heighten its antifungal potential, a combination of ten mutations in regulatory and synthesis pathway genes promotes 2-hydroxyphenazine synthesis in P. chlororaphis GP72 four-fold [ 179 ]. Changing glycerol utilization in this same microbe improves PCA production [ 160 ]. This effort is justified because of the success of the PCA-based pesticide “Shenqinmycin” and the availability of glycerol as a waste product from diesel formation [ 160 ].…”

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

“…Changing glycerol utilization in this same microbe improves PCA production [ 160 ]. This effort is justified because of the success of the PCA-based pesticide “Shenqinmycin” and the availability of glycerol as a waste product from diesel formation [ 160 ]. Other groups have modified different P. chlororaphis isolates to produce PCN and 1-hydroxyphenazines in order to enhance the range of plant fungal pathogens that are targeted by one isolate [ 180 , 181 ].…”

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

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Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

Lee¹,

Anderson

Kim

2022

Microorganisms

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Biological control is an important process for sustainable plant production, and this trait is found in many plant-associated microbes. This study reviews microbes that could be formulated into pesticides active against various microbial plant pathogens as well as damaging insects or nematodes. The focus is on the beneficial microbes that colonize the rhizosphere where, through various mechanisms, they promote healthy plant growth. Although these microbes have adapted to cohabit root tissues without causing disease, they are pathogenic to plant pathogens, including microbes, insects, and nematodes. The cocktail of metabolites released from the beneficial strains inhibits the growth of certain bacterial and fungal plant pathogens and participates in insect and nematode toxicity. There is a reinforcement of plant health through the systemic induction of defenses against pathogen attack and abiotic stress in the plant; metabolites in the beneficial microbial cocktail function in triggering the plant defenses. The review discusses a wide range of metabolites involved in plant protection through biocontrol in the rhizosphere. The focus is on the beneficial firmicutes and pseudomonads, because of the extensive studies with these isolates. The review evaluates how culture conditions can be optimized to provide formulations containing the preformed active metabolites for rapid control, with or without viable microbial cells as plant inocula, to boost plant productivity in field situations.

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“…Another commercial product is shenqinmycin, based on PCA. It has been in use since 2011 to control fungal diseases [ 160 ]. Metabolites from Streptomyces spp.…”

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

Section: Secondary Metabolites and Cell Preparations For Commercial B...mentioning

confidence: 99%

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Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

Lee¹,

Anderson

Kim

2022

Microorganisms

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“…2−4 The most typical phenazine is phenazine-1-carboxylic acid (PCA) registered as a novel biosynthetic fungicide "Shenqinmycin" by the Ministry of Agriculture of China in 2011. 3,5 PCA at a concentration of 1% showed highly effective activities in preventing pepper blight, rice sheath blight, and cucumber seedling damping-off. 6 2-Hydroxyphenazine (2-OH-PHZ) is another effective biocontrol antibiotic, which has a better inhibitory effect on the take-all disease of wheat than PCA.…”

Section: Introductionmentioning

confidence: 99%

“…Phenazines are a series of heterocyclic nitrogen-containing compounds, which are widely used in the field of biological control due to their high-efficiency broad-spectrum antifungal activity . Moreover, phenazines also exhibit the functions of anti-tumor, anti-cancer, and anti-parasitic effects, as an electron carrier, etc., attracting more and more researchers’ attention. − The most typical phenazine is phenazine-1-carboxylic acid (PCA) registered as a novel biosynthetic fungicide “Shenqinmycin” by the Ministry of Agriculture of China in 2011. , PCA at a concentration of 1% showed highly effective activities in preventing pepper blight, rice sheath blight, and cucumber seedling damping-off . 2-Hydroxyphenazine (2-OH-PHZ) is another effective biocontrol antibiotic, which has a better inhibitory effect on the take-all disease of wheat than PCA. , The take-all disease of wheat is one of the most serious diseases in the rhizosphere of wheat, and there is still a lack of effective control measures. , Therefore, 2-OH-PHZ is a promising candidate to develop a biopesticide to control the take-all disease of wheat.…”

Section: Introductionmentioning

confidence: 99%

Characterization and Engineering of Pseudomonas chlororaphis LX24 with High Production of 2-Hydroxyphenazine

Liu

Yue

Feng

et al. 2021

J. Agric. Food Chem.

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The take-all disease of wheat is one of the most serious diseases in the field of food security in the world. There is no effective biological pesticide to prevent the take-all disease of wheat. 2-Hydroxyphenazine (2-OH-PHZ) was reported to possess a better inhibitory effect on the take-all disease of wheat than phenazine-1-carboxylic acid, which was registered as “Shenqinmycin” in China in 2011. The aim of this study was to construct a 2-OH-PHZ high-producing strain by strain screening, genome sequencing, genetic engineering, and fermentation optimization. First, the metabolites of the previously screened new phenazine-producing Pseudomonas sp. strain were identified, and the taxonomic status of the new Pseudomonas sp. strain was confirmed through 16S rRNA and matrix-assisted laser desorption ionization–time-of-flight mass spectrometry (MALDI-TOF MS). Then, the new Pseudomonas sp. strain was named Pseudomonas chlororaphis subsp. aurantiaca LX24, which is a new subspecies of P. chlororaphis that can synthesize 2-OH-PHZ. Next, the draft genome of strain LX24 was determined, and clusters of orthologous group (COG) analysis, KEGG analysis, and gene ontology (GO) analysis of strain LX24 were performed. Furthermore, the production of 2-OH-PHZ increased to 351.7 from 158.6 mg/L by deletion of the phenazine synthesis negative regulatory genes rpeA and rsmE in strain LX24. Finally, the 2-OH-PHZ production of strain LX24 reached 677.1 mg/L after fermentation optimization, which is the highest production through microbial fermentation reported to date. This work provides a reference for the efficient production of other pesticides and antibiotics.

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Pseudomonas as Versatile Aromatics Cell Factory

Schwanemann

Otto

Wierckx

et al. 2020

Biotechnology Journal

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Aromatics and their derivatives are valuable chemicals with a plethora of important applications and thus play an integral role in modern society. Their current production relies mostly on the exploitation of petroleum resources. Independency from dwindling fossil resources and rising environmental concerns are major driving forces for the transition towards the production of sustainable aromatics from renewable feedstocks or waste streams. Whole‐cell biocatalysis is a promising strategy that allows the valorization of highly abundant, low‐cost substrates. In the last decades, extensive efforts are undertaken to allow the production of a wide spectrum of different aromatics and derivatives using microbes as biocatalysts. Pseudomonads are intriguing hosts for biocatalysis, as they display unique characteristics beneficial for the production of aromatics, including a distinct tolerance and versatile metabolism. This review highlights biotechnological applications of Pseudomonas as host for the production of aromatics and derived compounds. This includes their de novo biosynthesis from renewable resources, biotransformations in single‐ and biphasic fermentation setups, metabolic funneling of lignin‐derived aromatics, and the upcycling of aromatic monomers from plastic waste streams. Additionally, this review provides insights into unique features of Pseudomonads that make them exceptional hosts for aromatics biotechnology and discusses engineering strategies.

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Engineering of glycerol utilization in Pseudomonas chlororaphis GP72 for enhancing phenazine-1-carboxylic acid production

Cited by 21 publications

References 30 publications

Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

Root-Associated Bacteria Are Biocontrol Agents for Multiple Plant Pests

Characterization and Engineering of Pseudomonas chlororaphis LX24 with High Production of 2-Hydroxyphenazine

Pseudomonas as Versatile Aromatics Cell Factory

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