Engineering of
            <i>Deinococcus radiodurans</i>
            R1 for Bioprecipitation of Uranium from Dilute Nuclear Waste

Appukuttan, Deepti; Rao, A. T.; Apte, Shree Kumar

doi:10.1128/aem.02902-06

Cited by 25 publications

(39 citation statements)

References 26 publications

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“…Radionuclides can be immobilized through interactions between microbially-produced sulfide (White, Sharman, & Gadd, 1998;Lebranz et al, 2000) and phosphate (Macaskie et al, 1992;Boswell, Dick, & Macaskie, 1999;Jeong & Macaskie, 1999), or through bacterial iron oxidation (Banfield et al, 2000) in the general process of biomineralization (Martinez et al, 2007). Uranium phosphate precipitation has been facilitated by diverse bacterial genera including Arthrobacter, BacillusI, Rahnella, Deinococcus, Escherichia and Pseudomonas (Basnakova et al,24 1998; Powers et al, 2002;Appukuttan, Rao, & Apte, 2006). It has also been shown that Bacillus subtilis can immobilize U through the formation of uranyl-hydroxide, uranyl-carbonate, and calcium-uranyl-carbonate species with functional groups present on cell surfaces (Fowle, Fein, & Martin, 2000;Gorman-Lewis, Elias, & Fein, 2005).…”

Section: Radionuclide Bioprecipitation By Urease-producing Bacteriamentioning

confidence: 99%

Microbially-induced Carbonate Precipitation for Immobilization of Toxic Metals

Kumari

Qian

Pan

et al. 2016

Advances in Applied Microbiology

159

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Section: Radionuclide Bioprecipitation By Urease-producing Bacteriamentioning

confidence: 99%

Microbially-induced Carbonate Precipitation for Immobilization of Toxic Metals

Kumari

Qian

Pan

et al. 2016

Advances in Applied Microbiology

159

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“…Studies examining phosphatase activities (e.g., alkaline or acid) of genetically engineered and naturally occurring strains of Gram-positive and Gram-negative bacteria were shown to promote U immobilization (>90% precipitation of soluble U) via precipitation and coprecipitation reactions [50,57,60,74,[149][150][151][152][153]. Interestingly, soil bacterial isolates demonstrated constitutive phosphatase activities that liberated comparable, if not greater concentrations, of reactive phosphate when compared to the phosphatase activities of genetically modified strains [57,149].…”

Section: Phosphatase-mediated Biomineralizationmentioning

confidence: 99%

Phosphate-Mediated Remediation of Metals and Radionuclides

Martinez

Beazley

Sobecky

2014

Advances in Ecology

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Worldwide industrialization activities create vast amounts of organic and inorganic waste streams that frequently result in significant soil and groundwater contamination. Metals and radionuclides are of particular concern due to their mobility and longterm persistence in aquatic and terrestrial environments. As the global population increases, the demand for safe, contaminantfree soil and groundwater will increase as will the need for effective and inexpensive remediation strategies. Remediation strategies that include physical and chemical methods (i.e., abiotic) or biological activities have been shown to impede the migration of radionuclide and metal contaminants within soil and groundwater. However, abiotic remediation methods are often too costly owing to the quantities and volumes of soils and/or groundwater requiring treatment. The in situ sequestration of metals and radionuclides mediated by biological activities associated with microbial phosphorus metabolism is a promising and less costly addition to our existing remediation methods. This review highlights the current strategies for abiotic and microbial phosphatemediated techniques for uranium and metal remediation.

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“…Genome manipulation has been extensively used to clone and express genes encoding bioremediation functions in Deinococcus, for example, the use of merA to detoxify highly toxic, thiolreactive Hg(II), to much less toxic and nearly inert elemental and volatile Hg(0), 19 the tod and xyl operons to oxidize toluene and reduce Cr(VI) in sediment microcosms, 20 and phoN that is capable of bioprecipitation and biorecovery of uranium. 21 Previously, the S-layer lattices from D. radiodurans have been successfully used as a biotemplate for guided self-assembly of commercially procured hexagonal and honeycomb-ordered arrays of the dendrimer-encapsulated platinum nanoparticles, citrate-capped gold nanoparticles, and various species of CdSe/ ZnS core-shell quantum dots. 22 The present study involves the synthesis and extracellular accumulation of AgNPs using the bacterium D. radiodurans.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

Kulkarni¹,

Shaiwale²,

Deobagkar³

et al. 2015

IJN

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There has been rapid progress in exploring microorganisms for green synthesis of nanoparticles since microbes show extraordinary diversity in terms of species richness and niche localization. Microorganisms are easy to culture using relatively inexpensive and simple nutrients under varied conditions of temperature, pressure, pH, etc. In this work, Deinococcus radiodurans that possesses the ability to withstand extremely high radiation and desiccation stress has been employed for the synthesis of silver nanoparticles (AgNPs). D. radiodurans was able to accumulate AgNPs in medium under various conditions, and process optimization was carried out with respect to time, temperature, pH, and concentration of silver salt. AgNPs were characterized using UV/vis spectroscopy, scanning electron microscopy, transmission electron microscopy, X-ray diffraction, energy-dispersive X-ray spectroscopy, and Fourier transform infrared spectroscopy. The microbially synthesized AgNPs exhibited good antimicrobial activity against both Gram-negative and Gram-positive organisms and anti-biofouling activity. Their ability to inhibit growth and proliferation of cancer cell line was also examined, and it could be seen that AgNPs synthesized using D. radiodurans exhibited excellent anticancer activity.

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Engineering of Deinococcus radiodurans R1 for Bioprecipitation of Uranium from Dilute Nuclear Waste

Cited by 25 publications

References 26 publications

Microbially-induced Carbonate Precipitation for Immobilization of Toxic Metals

Microbially-induced Carbonate Precipitation for Immobilization of Toxic Metals

Phosphate-Mediated Remediation of Metals and Radionuclides

Synthesis and extracellular accumulation of silver nanoparticles by employing radiation-resistant Deinococcus radiodurans, their characterization, and determination of bioactivity

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