Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones

Li, Xuanyue; Xia, Jun; Nicolescu, Calin; Massidda, Miles W.; Ryan, Tyler; Tien, Joe

doi:10.1088/1758-5090/aae5fe

Cited by 24 publications

(22 citation statements)

References 37 publications

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“…In vitro microvascular systems have significant potential in three main areas, as highlighted in the excellent review in Reference 89: therapeutic vascularization of damaged tissue, vascularizing engineered tissues to create better implants (90), and microphysiological systems. In vitro microvascular systems that do not use blood have made enormous contributions to our…”

Section: Minding the Gap: Bridging Engineering And Microvascular Researchmentioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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Microengineering advances have enabled the development of perfusable, endothelialized models of the microvasculature that recapitulate the unique biological and biophysical conditions of the microcirculation in vivo. Indeed, at that size scale (<100 μm)—where blood no longer behaves as a simple continuum fluid; blood cells approximate the size of the vessels themselves; and complex interactions among blood cells, plasma molecules, and the endothelium constantly ensue—vascularized microfluidics are ideal tools to investigate these microvascular phenomena. Moreover, perfusable, endothelialized microfluidics offer unique opportunities for investigating microvascular diseases by enabling systematic dissection of both the blood and vascular components of the pathophysiology at hand. We review ( a) the state of the art in microvascular devices and ( b) the myriad of microvascular diseases and pressing challenges. The engineering community has unique opportunities to innovate with new microvascular devices and to partner with biomedical researchers to usher in a new era of understanding and discovery of microvascular diseases. Expected final online publication date for the Annual Review of Biomedical Engineering, Volume 23 is June 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

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Section: Minding the Gap: Bridging Engineering And Microvascular Researchmentioning

confidence: 99%

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Myers

Lam

2021

Annu. Rev. Biomed. Eng.

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“…Solute permeability tends to be comparable to those of explanted microvessels and up to an order-of-magnitude higher than those observed in vivo. Vessels in these gels can also be used to perfuse microscale biomaterials and tissues (64,246,340). Skin equivalents that incorporate vessels can be used to study vascular absorption of topically applied substances (390).…”

Section: Decellularization Of Organsmentioning

confidence: 99%

“…Skin equivalents that incorporate vessels can be used to study vascular absorption of topically applied substances (390). The addition of adipocytes to the gel increased vascular permeability (340).…”

Section: Decellularization Of Organsmentioning

confidence: 99%

Tissue Engineering of the Microvasculature

Tien

2019

Comprehensive Physiology

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The ability of generate new microvessels in desired numbers and at desired locations has been a long-sought goal in vascular medicine, engineering, and biology. Historically, the need to revascularize ischemic tissues non-surgically (so-called therapeutic vascularization) served as the main driving force for the development of new methods of vascular growth. More recently, vascularization of engineered tissues and the generation of vascularized microphysiological systems have provided additional targets for these methods, and have required adaptation of therapeutic vascularization to biomaterial scaffolds and to microscale devices. Three complementary strategies have been investigated to engineer microvasculature: angiogenesis (the sprouting of existing vessels), vasculogenesis (the coalescence of adult or progenitor cells into vessels), and microfluidics (the vascularization of scaffolds that possess the open geometry of microvascular networks). Over the past several decades, vascularization techniques have grown tremendously in sophistication, from the crude implantation of arteries into myocardial tunnels by Vineberg in the 1940s, to the current use of micropatterning techniques to control the exact shape and placement of vessels within a scaffold. This review provides a broad historical view of methods to engineer the microvasculature, and offers a common framework for organizing and analyzing the numerous studies in this area of tissue engineering and regenerative medicine.

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“…Then hydrogel was added into the reservoir and the stainless‐steel needle was pulled out after the gel was set. The PDMS‐based chip allowed robust perfusion of supplied nutrition in the casted hydrogel . Li et al demonstrated the engineering of a vascularized adipose tissue by casting collagen gel with adipocytes in the reservoir and vascularizing the channel with ECs.…”

Section: Engineering Approaches On Fabricating Perfusable Microchannementioning

confidence: 99%

“…Li et al demonstrated the engineering of a vascularized adipose tissue by casting collagen gel with adipocytes in the reservoir and vascularizing the channel with ECs. With the constant and robust perfusion, the adipose tissue could be remained viable and respond to hormone stimuluses with lipid accumulation or loss . Mannino et al modeled a complex tumor microenvironment by constructing a perfusable and vascularized lumen within the hyaluronic acid‐based hydrogel.…”

Section: Engineering Approaches On Fabricating Perfusable Microchannementioning

confidence: 99%

Engineering of Hydrogel Materials with Perfusable Microchannels for Building Vascularized Tissues

Xie

Zheng

Guan

et al. 2019

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129

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Vascular systems are responsible for various physiological and pathological processes related to all organs in vivo, and the survival of engineered tissues for enough nutrient supply in vitro. Thus, biomimetic vascularization is highly needed for constructing both a biomimetic organ model and a reliable engineered tissue. However, many challenges remain in constructing vascularized tissues, requiring the combination of suitable biomaterials and engineering techniques. In this review, the advantages of hydrogels on building engineered vascularized tissues are discussed and recent engineering techniques for building perfusable microchannels in hydrogels are summarized, including micromolding, 3D printing, and microfluidic spinning. Furthermore, the applications of these perfusable hydrogels in manufacturing organ‐on‐a‐chip devices and transplantable engineered tissues are highlighted. Finally, current challenges in recapitulating the complexity of native vascular systems are discussed and future development of vascularized tissues is prospected.

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Engineering of microscale vascularized fat that responds to perfusion with lipoactive hormones

Cited by 24 publications

References 37 publications

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Vascularized Microfluidics and Their Untapped Potential for Discovery in Diseases of the Microvasculature

Tissue Engineering of the Microvasculature

Engineering of Hydrogel Materials with Perfusable Microchannels for Building Vascularized Tissues

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