Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Nagasaki, Yukio

doi:10.1088/1468-6996/11/5/054505

Cited by 21 publications

(8 citation statements)

References 82 publications

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“…We have already confirmed the usefulness of the oligoamine anchor 15,16,26,27,56,[72][73][74][75][76] instead of the sulfanyl group anchor [77][78][79] for molecular immobilization on gold surfaces and developed polyamineended PEG-modified gold surfaces using PEG-block-poly [2- Densely PEG-chain-tethered biointerface Y Nagasaki platform for ssDNA immobilization and detection; 73 in our scheme, PEG-b-PAMA was immobilized onto the gold surface followed by treatment with a ssDNA-SH, resulting in the formation of ssDNA-SH/ PEG-b-PAMA co-immobilized gold surface.…”

Section: Construction Of Oligodna/peg Hybrid Surfaces and Their Perfosupporting

confidence: 64%

“…The present review describes the effect of mixed PEG on the non-biofouling character and also hybrid construction of a PEG/biopolymer mixture on substrate surfaces for versatile applications. Because many excellent reviews on PEG surface coating have been previously published, [15][16][17][18][19][20][21][22][23][24][25][26][27][28] this review mainly introduces our work. BACKGROUND PEG has long been manufactured industrially and utilized in many applications such as nonionic surfactants, lubricants, an intermediate for urethane composition, adhesives and cosmetics.…”

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confidence: 99%

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Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

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116

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Non-biofouling surfaces constitute one of the most important subjects in sensitively and selectively detecting biomolecular events. Poly(ethylene glycol) (PEG) chains tethered on substrate surfaces are well known to reduce non-biofouling characteristics. Protein adsorption onto a PEG-chain-tethered surface is strongly influenced by the density of the PEG chain and is almost completely suppressed by the successive treatment of longer PEG chains (5 kDa) followed by the treatment of PEG (2 kDa; mixed-PEG-chain-tethered surface) because of a significant increase in PEG chain density. To modify versatile substrate surface, PEG possessing pentaethylenehexamine at one end (N6-PEG) was prepared via a reductive amination reaction of aldehyde-ended PEG with pentaethylenehexamine. Using N6-PEG, antibody/PEG co-immobilization was conducted on a substrate possessing active ester groups. After the antibody was immobilized on the surface, PEG tethered chains were constructed surrounding the immobilized antibody. It is interesting to note that the PEG-chain-tethered functions not only as a non-fouling agent but also improves immune response. The hybrid surface was also applied to oligo DNA immobilization. The oligo DNA/PEG hybrid surface improved hybridization, retaining its non-fouling ability. Densely packed PEG tethered chains surrounding antibodies and/or oligo DNA improved their orientation on the surface. Thus, this material is promising as a high-performance biointerface for versatile applications. Keywords: antibody; biosensing; DNA; end-functionalized poly(ethylene glycol); enzyme-linked immunosorbent assay; hybrid biointerface; soft interface INTRODUCTION Specific biorecognition on substrate surfaces has long been utilized in many applications such as biosensing, 1 immunodiagnostics, 2 enzyme immunoassay, 3 western blotting 4 and protein microarrays. 5 Important factors for the improvement in specific biorecognition on surfaces are the suppression of nonspecific biofouling and the suitable orientation of immobilized biomolecules. The former decreases nonspecific noise and the background level, and the latter increases the specific recognition level. To improve the non-biofouling character of surfaces, numerous efforts have been undertaken. In general, natural polymers such as albumin, casein and dextran have been used for blocking on substrate surfaces. 6 These treatments work well and suppress protein biofouling extensively. If extremely high performance is required, however, these blocking treatments are not enough. In addition, natural products, especially those derived from animals, may have undesired contents such as bacteria and viruses, which may affect the user. 7 Under these circumstances, synthetic polymers have been recently suggested as suitable surface blocking agents. Numerous types of water-soluble polymers, such as poly(acrylamide), poly(vinyl alcohol), poly(vinyl pyrrolidone) and poly(ethylene glycol) (PEG), have been investigated so far. Recently, new types of synthetic polymers such as poly(methoxy ...

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Section: Construction Of Oligodna/peg Hybrid Surfaces and Their Perfosupporting

confidence: 64%

mentioning

confidence: 99%

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Nagasaki

2011

Polym J

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116

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“…Similarly, only a trace amount of TBA was immobilized on the pentaethylenehexamine-terminated PEG (N6-PEG) surface owing to the high density of PEG chains (Figure b). However, TBAs were immobilized on the gold surface after the modification of PEG- b -PAMA (Figure c–e) The amino groups in the PAMA chain show a strong affinity for the gold atoms, mainly through coordination of the lone pair of the amino group. , We confirmed previously that the chain density of tethered PEG can be controlled by the number of amino groups in the PAMA segment. According to a previous report, the density of PEG- b -PAMA(43) is less than half that of PEG- b -PAMA(3) .…”

Section: Resultssupporting

confidence: 84%

High-Performance Surface Acoustic Wave Immunosensing System on a PEG/Aptamer Hybridized Surface

Horiguchi

Miyachi²,

Nagasaki

2013

Langmuir

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Label-free immunoassay systems have the advantages of procedural simplicity and a low construction cost of surfaces for immunosensing. When label-free immunoassay systems are considered, the nonspecific adsorption of unwanted materials should be eliminated unless it aids in the detection of error. PEG is well-known as a blocking agent for the prevention of the adsorption of nonspecific binding materials when coimmobilized with ligands for targets such as antibodies and oligonucleotides. The construction strategy for PEG/ligand coimmobilized surfaces is an important point in the preparation of a high-performance assays because the physiological condition of the ligand depends strongly on its interaction with the PEG chain. In this report, we investigate the interaction between thrombin and a thrombin-binding aptamer (TBA) on a PEG/TBA coimmobilized surface by using a shear horizontal surface acoustic wave (SAW) sensor. The thrombin-TBA binding property shows remarkable differences with changes in the PEG density and the distance from the gold surface to the aptamer.

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“…The bidentate thiols bind to the QD surface strongly and helps contribute to QD dispersions that are stable in aqueous solutions for long periods of time by alleviating many of the issues associated with the dynamic off-rate that monothiolated ligands typically experience. ,, The majority of the ligands then display a poly(ethylene glycol) or PEG linker of ∼13 ethylene oxide repeats except for the methoxy ligand which has ∼16 repeats. PEG is the preferred molecule for mediating colloidal stability in many established and developing NP and nanomedicine applications due to it is generally accepted hydrophilic, inert and nonfouling character. − The ligand set then terminates in different functional groups including an amine (NH 2 ), acetyl (COCH 3 ), methoxy (OMe), hydroxy (OH), carboxy (COOH), and zwitterionic group (CL4/PEG-CL4), see Figure and Table . Theses names/designations are used somewhat interchangeably throughout.…”

Section: Resultsmentioning

confidence: 99%

Elucidating Surface Ligand-Dependent Kinetic Enhancement of Proteolytic Activity at Surface-Modified Quantum Dots

et al. 2017

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Combining biomolecules such as enzymes with nanoparticles has much to offer for creating next generation synergistically functional bionanomaterials. However, almost nothing is known about how these two disparate components interact at this critical biomolecular-materials interface to give rise to improved activity and emergent properties. Here we examine how the nanoparticle surface can influence and increase localized enzyme activity using a designer experimental system consisting of trypsin proteolysis acting on peptide-substrates displayed around semiconductor quantum dots (QDs). To minimize the complexity of analyzing this system, only the chemical nature of the QD surface functionalizing ligands were modified. This was accomplished by synthesizing a series of QD ligands that were either positively or negatively charged, zwitterionic, neutral, and with differing lengths. The QDs were then assembled with different ratios of dye-labeled peptide substrates and exposed to trypsin giving rise to progress curves that were monitored by Förster resonance energy transfer (FRET). The resulting trypsin activity profiles were analyzed in the context of detailed molecular dynamics simulations of key interactions occurring at this interface. Overall, we find that a combination of factors can give rise to a localized activity that was 35-fold higher than comparable freely diffusing enzyme-substrate interactions. Contributing factors include the peptide substrate being prominently displayed extending from the QD surface and not sterically hindered by the longer surface ligands in conjunction with the presence of electrostatic and other productive attractive forces between the enzyme and the QD surface. An intimate understanding of such critical interactions at this interface can produce a set of guidelines that will allow the rational design of next generation high-activity bionanocomposites and theranostics.

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Engineering of poly(ethylene glycol) chain-tethered surfaces to obtain high-performance bionanoparticles

Cited by 21 publications

References 82 publications

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

Construction of a densely poly(ethylene glycol)-chain-tethered surface and its performance

High-Performance Surface Acoustic Wave Immunosensing System on a PEG/Aptamer Hybridized Surface

Elucidating Surface Ligand-Dependent Kinetic Enhancement of Proteolytic Activity at Surface-Modified Quantum Dots

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