Engineering of Secondary Metabolites in Tissue and Cell Culture of Medicinal Plants: An Alternative to Produce Beneficial Compounds Using Bioreactor Technologies

Ebrahimi, Mortaza; Mokhtari, Arash

doi:10.1007/978-3-319-65079-1_7

Cited by 6 publications

(1 citation statement)

References 142 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Presently, plants are widely used in industry for the production of different kinds of primary and secondary metabolites, which are used as dyes, fragrances, food additives, insecticides, or drugs (Hussain et al 2012). Plant cell and tissue culture systems in bioreactors are commonly used in the production of such active compounds (Ebrahimi and Mokhtari 2017). However, to be able to synthesize secondary metabolites in tissue cultures, appropriate techniques need to be developed and work efficiently.…”

Section: Introductionmentioning

confidence: 99%

Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.

Klimek-Chodacka

Kadluczka

Łukasiewicz

et al. 2020

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

In this study we report the development of effective in vitro systems for a medicinal plant Nigella damascena L. comprising: (1) callus induction, (2) somatic embryogenesis in callus cultures with subsequent plant regeneration, and (3) isolation and regeneration of callus-derived protoplasts. Callus development was achieved on 83–100% of hypocotyl and cotyledon explants, whereby Murashige and Skoog medium (MS) supplemented with 3 mg L−1 6-benzylaminopurine and 0.5 mg L−1α-naphthaleneacetic acid (NAA; BN medium) was more advantageous than MS with kinetin and NAA (KN medium). Histological observations of calli revealed the presence of embryogenic zones from which somatic embryos developed on the hormone-free medium. Plant regeneration was observed on 76–95% of calli. A high capacity to form somatic embryos and regeneration was maintained in long-lasting cultures, i.e. even in 2 year old callus. The obtained callus was also a good source tissue for protoplast isolation. By applying a mixture of cellulase and pectolyase, the acceptable yield of viable protoplasts was achieved, especially from hypocotyl-derived callus maintained on BN medium. Protoplasts embedded in an alginate matrix and cultured in modified Kao and Michayluk media re-constructed their cell wall and re-entered mitotic divisions. About 30% of small cell aggregates formed microcalli, which, after the release from alginate, proliferated continuously on KN and BN media, irrespective of the tissue variant used as the protoplast source. Somatic embryo formation and plant regeneration were successful on hormone-free media. An effective plant regeneration system of N. damascena protoplast cultures has been developed and is being reported for the first time.

show abstract

Section: Introductionmentioning

confidence: 99%