Engineering phosphatidylinositol-4,5-bisphosphate model membranes enriched in endocytic cargo: A neutron reflectometry, AFM and QCM-D structural study

Pereira, Daniel; Santamaria, Andreas; Pawar, Nisha; Carrascosa-Tejedor, Javier; Sardo, Mariana; Mafra, Luís; Guzmán, Eduardo; Owen, David J.; Zaccai, Nathan R.; Maestro, Armando; Marín-Montesinos, Ildefonso

doi:10.1016/j.colsurfb.2023.113341

Cited by 4 publications

(3 citation statements)

References 51 publications

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“…Briefly, a shear stress applied to the film by the oscillating crystal results in both elastic (spring) and viscous (dashpot) contributions to the stress/strain relation, which can be represented by eq 1 σ x y = μ ∂ u x ( y , t ) ∂ y + η ∂ v x ( y , t ) ∂ y where μ is the shear modulus of the film, η is the shear viscosity of the film, and u x and v x represent the displacement and velocity in the x -direction, respectively. Using the Taylor expansion, the film viscoelastic properties [including viscosity, density, shear modulus, and thickness (mass)] can be obtained by fitting the measured Δ F and Δ D to eqs and at different overtones , | normalΔ F | ≅ 1 2 π ρ q t q t f ρ f ω ( 1 + 2 t normalf 2 χ 3 δ 2 false( 1 + χ 2 false) ) normalΔ D ≅ 2 t 3 ρ f f 3 π f 0 ρ q t q 1 δ 2 …”

Section: Methodsmentioning

confidence: 99%

“…where μ is the shear modulus of the film, η is the shear viscosity of the film, and u x and v x represent the displacement and velocity in the xdirection, respectively. Using the Taylor expansion, the film viscoelastic properties [including viscosity, density, shear modulus, and thickness (mass)] can be obtained by fitting the measured ΔF and ΔD to eqs 3 and 4 at different overtones 42,43…”

Section: = + U Y T Y V Y T Ymentioning

confidence: 99%

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Synthesis of Raspberry-like Nanoparticles via Surface Grafting of Positively Charged Polyelectrolyte Brushes: Colloidal Stability and Surface Properties

Aldakkan,

Chalmpes,

et al. 2024

Langmuir

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A method to synthesize stable, raspberry-like nanoparticles (NPs), using surface grafting of poly(glycidyl methacrylate) (PGMA) brushes on a polystyrene (PS) core with varying grafting densities, is reported. A twostep functionalization reaction of PGMA epoxide groups comprising an amination step first using ethylene diamine and then followed by a quaternization using glycidyltrimethylammonium chloride generates permanently and positively charged polyelectrolyte brushes, which result in both steric and electrostatic stabilization. The dispersion stability of the brush-bearing NPs is dramatically improved compared to that of the pristine PS core in salt solutions at ambient (25 °C) and elevated temperatures (60 °C). Additionally, the grafted polyelectrolyte chains undergo a reversible swelling in the presence of different ionic strength (IS) salts, which modulate the surface properties, including roughness, stiffness, and adhesion. An atomic force microscope under both dry and wet conditions was used to image conformational changes of the polyelectrolyte chains during the swelling and deswelling transitions as well as to probe the nanomechanical properties by analyzing the corresponding force−sample separation curves. The quaternized polyelectrolyte brushes undergo a conformational transition from a collapsed state to a swelled state in the osmotic brush (OB) regime triggered by the osmotic gradient of mobile ions to the interior of the polymer chain. At IS ∼ 1 M, the brushes contract and the globules reform (salted brush state) as evidenced by an increase in the surface roughness and a reduction in the adhesion of the brushes. Beyond IS ∼ 1 M, quartz crystal microbalance with dissipation monitoring measurements show that salt uptake continues to take place predominantly on the exterior surface of the brush since salt adsorption is not accompanied by a size increase as measured by dynamic light scattering. The study adds new insights into our understanding of the behavior of NPs bearing salt-responsive polyelectrolyte brushes with adaptive swelling thresholds that can ultimately modulate surface properties.

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Section: Methodsmentioning

confidence: 99%

Section: = + U Y T Y V Y T Ymentioning

confidence: 99%

Synthesis of Raspberry-like Nanoparticles via Surface Grafting of Positively Charged Polyelectrolyte Brushes: Colloidal Stability and Surface Properties

Aldakkan,

Chalmpes,

et al. 2024

Langmuir

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“…Techniques such as Brewster Angle Microscopy (BAM), Atomic Force Microscopy (AFM), Surface Force Apparatus (SFA), Ellipsometry, Infrared Reflection Absorption Spectroscopy (IRRAS), or Epifluorescence Microscopy, and their combinations with tensiometric techniques (mainly Langmuir film balances), are powerful to study the changes in biological relevant film organization at micrometric and submicrometric scales [69][70][71][72]. Advanced tools such as neutron reflectivity or synchrotron grazing angle X-ray diffraction can also help to evaluate the organization of biologically relevant interfacial films [39,[73][74][75][76][77][78][79][80][81][82][83][84].…”

Section: Surface Science Methods For Studying Biophysical Relevant Sy...mentioning

confidence: 99%

Fluid Interfaces as Models for the Study of Lipid-Based Films with Biophysical Relevance

Argudo,

Maestro,

Guzmán

2023

Coatings

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This comprehensive review aims to provide a deep insight into the fascinating field of biophysics in living organisms through the study of model fluid interfaces that mimic specific lipid-based structures with biophysical relevance. By delving into the study of these physiological fluid interfaces, we can unravel crucial aspects of their behavior, lateral organization, and functions. Through specific examples, we will uncover the intricate dynamics at play and shed light on potential pathogenic conditions that may result from alterations in these interfaces. A deeper understanding of these aspects can lead to the emergence of novel technologies and medical advances, potentially leading to the development of innovative treatments and diagnostic tools.

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Exploring the Interaction of Lipid Bilayers with Curcumin–Laponite Nanoparticles: Implications for Drug Delivery and Therapeutic Applications

Pawar,

Peña‐Figueroa,

Verde‐Sesto

et al. 2024

Small

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Curcumin, the active compound in turmeric, is renowned for its anti‐inflammatory, antioxidant, and antimicrobial properties, making it beneficial for treating conditions like arthritis, neurodegenerative diseases, and various cancers. Despite its promising therapeutic potential, curcumin's poor bioavailability—due to its rapid metabolism and low solubility—limits its clinical efficacy. To address this, recent research has focused on enhancing curcumin delivery using nanoparticles, liposomes, and novel nanomaterials. Among these, laponite, a synthetic nanoclay, has shown promise in improving curcumin delivery due to its unique properties, including large surface area, dual charge, and stability in solution. This study explores the use of curcumin–laponite nanoparticles as carrier vehicles for controlled delivery to in vitro model membranes. Utilizing advanced techniques such as neutron reflectometry, atomic force microscopy, quartz crystal microbalance with dissipation, and infrared spectroscopy, the interaction between curcumin–laponite nanoparticles and solid‐supported lipid bilayers is monitored, revealing enhanced stability and controlled release of curcumin across the membrane. These findings pave the way for the development of curcumin‐based therapies targeting cardiovascular, neurological, and oncological diseases, leveraging the synergistic effects of curcumin's biological activity and laponite's delivery capabilities.

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Engineering phosphatidylinositol-4,5-bisphosphate model membranes enriched in endocytic cargo: A neutron reflectometry, AFM and QCM-D structural study

Cited by 4 publications

References 51 publications

Synthesis of Raspberry-like Nanoparticles via Surface Grafting of Positively Charged Polyelectrolyte Brushes: Colloidal Stability and Surface Properties

Synthesis of Raspberry-like Nanoparticles via Surface Grafting of Positively Charged Polyelectrolyte Brushes: Colloidal Stability and Surface Properties

Fluid Interfaces as Models for the Study of Lipid-Based Films with Biophysical Relevance

Exploring the Interaction of Lipid Bilayers with Curcumin–Laponite Nanoparticles: Implications for Drug Delivery and Therapeutic Applications

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