Engineering photo-methylotrophic Methylobacterium for enhanced 3-hydroxypropionic acid production during non-growth stage fermentation

Ma, Zeng-Xin; Feng, Chen-Xi; Song, Ya-Zhen; Sun, Jing; Shao, Yi; Song, Shu-Zhen; Wan, Bin; Zhang, Cong; Fan, Huan; Bao, Kai; Yang, Song

doi:10.1016/j.biortech.2023.130104

Cited by 2 publications

(1 citation statement)

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“…PHB extraction and analysis were performed following previously established protocols with slight modifications 72 . The instrumental analysis employed a GC-MS system (GC17A coupled with a QP-2020 mass spectrometer, Shimadzu, Kyoto, Japan) equipped with an SH-Rxi-5Sil MS capillary column (30 m × 0.25 mm × 0.25 μm, Shimadzu, Japan).…”

Section: Methodsmentioning

confidence: 99%

Phosphoribosylpyrophosphate synthetase as a metabolic valve advances Methylobacterium/Methylorubrum phyllosphere colonization and plant growth

Zhang,

Zhou,

Wang

et al. 2024

Nat Commun

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The proficiency of phyllosphere microbiomes in efficiently utilizing plant-provided nutrients is pivotal for their successful colonization of plants. The methylotrophic capabilities of Methylobacterium/Methylorubrum play a crucial role in this process. However, the precise mechanisms facilitating efficient colonization remain elusive. In the present study, we investigate the significance of methanol assimilation in shaping the success of mutualistic relationships between methylotrophs and plants. A set of strains originating from Methylorubrum extorquens AM1 are subjected to evolutionary pressures to thrive under low methanol conditions. A mutation in the phosphoribosylpyrophosphate synthetase gene is identified, which converts it into a metabolic valve. This valve redirects limited C1-carbon resources towards the synthesis of biomass by up-regulating a non-essential phosphoketolase pathway. These newly acquired bacterial traits demonstrate superior colonization capabilities, even at low abundance, leading to increased growth of inoculated plants. This function is prevalent in Methylobacterium/Methylorubrum strains. In summary, our findings offer insights that could guide the selection of Methylobacterium/Methylorubrum strains for advantageous agricultural applications.

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Section: Methodsmentioning

confidence: 99%