Engineering photoautotrophic carbon fixation for enhanced growth and productivity

Liang, Feiyan; Lindberg, Pia; Lindblad, Peter

doi:10.1039/c8se00281a

Cited by 52 publications

(38 citation statements)

References 114 publications

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“…The upregulation of ROS quenching capability could be important, as ROS may deleteriously affect cellular metabolic processes, such as nutrient bioavailability, photosynthesis and carbon flux, in plants [54]. With the decreased H 2 O 2 content and increased cell growth of S. elongatus UTEX 2973, we also evaluated the expression levels of several genes involved in photosynthesis, including the carbon dioxide-concentrating mechanism protein-coding gene ccmM [55], ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)coding gene rbcL [56], PSII-related subunit-coding genes cp43 and cp47, PSI essential subunits of reaction centercoding genes psaA and psaB [57], and chlorophyll a synthesis-related genes chlaA and pcrA [58] (Additional file 5: Table S1); as expected, all these genes were upregulated (Additional file 7: Fig. S6).…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Zhang

Chen

Diao

et al. 2020

Biotechnol Biofuels

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Background: Cyanobacterial carbohydrates, such as sucrose, have been considered as potential renewable feedstock to support the production of fuels and chemicals. However, the separation and purification processes of these carbohydrates will increase the production cost of chemicals. Co-culture fermentation has been proposed as an efficient and economical way to utilize these cyanobacterial carbohydrates. However, studies on the application of co-culture systems to achieve green biosynthesis of platform chemicals are still rare. Results: In this study, we successfully achieved one-step conversion of sucrose derived from cyanobacteria to fine chemicals by constructing a microbial consortium consisting of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 and Escherichia coli to sequentially produce sucrose and then the platform chemical 3-hydroxypropionic acid (3-HP) from CO 2 under photoautotrophic growth conditions. First, efforts were made to overexpress the sucrose permease-coding gene cscB under the strong promoter P cpc560 in S. elongatus UTEX 2973 for efficient sucrose secretion. Second, the sucrose catabolic pathway and malonyl-CoA-dependent 3-HP biosynthetic pathway were introduced into E. coli BL21 (DE3) for heterologous biosynthesis of 3-HP from sucrose. By optimizing the cultivation temperature from 37 to 30 °C, a stable artificial consortium system was constructed with the capability of producing 3-HP at up to 68.29 mg/L directly from CO 2. In addition, cell growth of S. elongatus UTEX 2973 in the consortium was enhanced, probably due to the quick quenching of reactive oxygen species (ROS) in the system by E. coli, which in turn improved the photosynthesis of cyanobacteria. Conclusion: The study demonstrated the feasibility of the one-step conversion of sucrose to fine chemicals using an artificial consortium system. The study also confirmed that heterotrophic bacteria could promote the cell growth of cyanobacteria by relieving oxidative stress in this microbial consortium, which further suggests the potential value of this system for future industrial applications.

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Section: Discussionmentioning

confidence: 99%

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Zhang

Chen

Diao

et al. 2020

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…The upregulation of ROS quenching capability could be important, as ROS may deleteriously affect cellular metabolic processes, such as nutrient bioavailability, photosynthesis and carbon flux, in plants [54]. With the decreased H 2 O 2 content and increased cell growth of S. elongatus UTEX 2973, we also evaluated the expression levels of several genes involved in photosynthesis, including the carbon dioxide-concentrating mechanism protein-coding gene ccmM [55], ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO)-coding gene rbcL [56], PSII related subunit-coding genes cp43 and cp47, PSI essential subunits of reaction center-coding genes psaA and psaB [57], and chlorophyll a synthesis-related genes chlaA and pcrA [58] (Suppl. Table.…”

Section: Discussionmentioning

confidence: 99%

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Zhang

Chen

Diao

et al. 2020

Preprint

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Background Cyanobacterial carbohydrates, such as sucrose, have been considered as potential renewable feedstock to support the production of fuels and chemicals. However, the separation and purification processes of these carbohydrates will increase the production cost of chemicals. Co-culture fermentation has been proposed as an efficient and economical way to utilize these cyanobacterial carbohydrates. However, studies on the application of co-culture systems to achieve green biosynthesis of platform chemicals are still rare. Results In this study, we successfully achieved one-step conversion of sucrose derived from cyanobacteria to fine chemicals by constructing a microbial consortium consisting of the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 and Escherichia coli to sequentially produce sucrose and then the platform chemical 3-hydroxypropionic acid (3-HP) from CO 2 under photoautotrophic growth conditions. First, efforts were made to overexpress the sucrose permease-coding gene cscB under the strong promoter P cpc560 in S. elongatus UTEX 2973 for efficient sucrose secretion. Second, the sucrose catabolic pathway and malonyl CoA-dependent 3-HP biosynthetic pathway were introduced into E. coli BL21(DE3) for heterologous biosynthesis of 3-HP from sucrose. By optimizing the cultivation temperature from 37℃ to 30℃, a stable artificial consortium system was constructed with the capability of producing 3-HP at up to 68.29 mg/L directly from CO 2 . In addition, cell growth of S. elongatus UTEX 2973 in the consortium was enhanced, probably due to the quick quenching of reactive oxygen species (ROS) in the system by E. coli , which in turn improved the photosynthesis of cyanobacteria. Conclusion The study demonstrated the feasibility of the one-step conversion of sucrose to fine chemicals using an artificial consortium system. The study also confirmed that heterotrophic bacteria could promote the cell growth of cyanobacteria by relieving oxidative stress in this microbial consortium, which further suggests the potential value of this system for future industrial applications.

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“…In the carbon-fixation pathway, many DEGs exhibited higher expression in both the hybrids from one of the parental lines directly related to yield, e.g., glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Ribulose-Bisphosphate Carboxylase (RuBisCO), malate dehydrogenase (MDH), pyruvate orthophosphate dikinase (PPDK), and fructose-1,6-bisphosphatase (FBP). For instance, RuBisCO (Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase) is the key enzyme for CO 2 fixation in a rice plant and subsequent formation of starch and sucrose [27]. The same enzyme is also responsible for photorespiratory loss of CO 2 .…”

Section: Photosynthesis and Yield In Hybrid Ricementioning

confidence: 99%

Differential Expression of Genes at Panicle Initiation and Grain Filling Stages Implied in Heterosis of Rice Hybrids

Katara

Verma

Parida

et al. 2020

IJMS

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RNA-Seq technology was used to analyze the transcriptome of two rice hybrids, Ajay (based on wild-abortive (WA)-cytoplasm) and Rajalaxmi (based on Kalinga-cytoplasm), and their respective parents at the panicle initiation (PI) and grain filling (GF) stages. Around 293 and 302 million high quality paired-end reads of Ajay and Rajalaxmi, respectively, were generated and aligned against the Nipponbare reference genome. Transcriptome profiling of Ajay revealed 2814 and 4819 differentially expressed genes (DEGs) at the PI and GF stages, respectively, as compared to its parents. In the case of Rajalaxmi, 660 and 5264 DEGs were identified at PI and GF stages, respectively. Functionally relevant DEGs were selected for validation through qRT-PCR, which were found to be co-related with the expression patterns to RNA-seq. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated significant DEGs enriched for energy metabolism pathways, such as photosynthesis, oxidative phosphorylation, and carbon fixation, at the PI stage, while carbohydrate metabolism-related pathways, such as glycolysis and starch and sucrose metabolism, were significantly involved at the GF stage. Many genes involved in energy metabolism exhibited upregulation at the PI stage, whereas the genes involved in carbohydrate biosynthesis had higher expression at the GF stage. The majority of the DEGs were successfully mapped to know yield related rice quantitative trait loci (QTLs). A set of important transcription factors (TFs) was found to be encoded by the identified DEGs. Our results indicated that a complex interplay of several genes in different pathways contributes to higher yield and vigor in rice hybrids.

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Engineering photoautotrophic carbon fixation for enhanced growth and productivity

Abstract: This review discusses strategies to enhance oxygenic photoautotrophic carbon fixation with a focus on the Calvin–Benson–Bassham cycle.

Cited by 52 publications

References 114 publications

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Construction and analysis of an artificial consortium based on the fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 to produce the platform chemical 3-hydroxypropionic acid from CO2

Differential Expression of Genes at Panicle Initiation and Grain Filling Stages Implied in Heterosis of Rice Hybrids

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