Engineering plant microbiomes by integrating eco-evolutionary principles into current strategies

Moreira, Zayda Piedad Morales; Chen, Melissa Y.; Ortuno, Daniela L. Yanez; Haney, Cara H.

doi:10.1016/j.pbi.2022.102316

Cited by 32 publications

(20 citation statements)

References 91 publications

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“…Future research should embrace the seemingly chaotic qualities of microbial communities and leverage the current wave of novel high-throughput tools to better resolve the underlying drivers of stochasticity in difficult datasets. Doing so will facilitate the translation of lab results into practical and effective approaches in microbiome engineering that can help combat pathogens in diverse host systems 21,22 .…”

Section: Discussionmentioning

confidence: 99%

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Order among chaos: high throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system

Chen,

Fulton,

Huang

et al. 2024

Preprint

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While microbiomes provide diverse benefits for their host, they are notoriously variable in structure and function. As a result, substantial experimental replication and scalability are required to identify the contributions of and interactions between microbiota, the host and the environment. Here, we describe a novel high throughput plant growth system (MYCroplanters) to test how multiple host, microbiota, and pathogen variables predict host health. Using an Arabidopsis-Pseudomonas host-microbiome-pathogen model, we found that host genotype and order of arrival predict competition outcome between strains in the rhizosphere, but pathogen and microbiota dose can overwhelm these effects. Regardless of host or inoculation conditions, final microbial community structure emerged as a consistent predictor of host health. We demonstrate that high-throughput tools like MYCroplanters can isolate interacting drivers of host health. Increasing the scale at which we can screen components of microbiome-mediated host benefits will facilitate building microbiome engineering solutions for medicine and agricultural applications.

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Section: Discussionmentioning

confidence: 99%

“…These data indicate that with sufficient throughput, multiple interacting variables can be isolated in highly complex systems. Dissecting contributions of individual variables can determine which are most important in predicting health outcomes, which will facilitate microbiome engineering for agriculture 21 or medicine 22 .…”

Section: Introductionmentioning

confidence: 99%

Order among chaos: high throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system

Chen,

Fulton,

Huang

et al. 2024

Preprint

Self Cite

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“…Incorporating microbial inoculants in agricultural technologies may aid in achieving viable soil properties. Bio‐sequestration refers to the biological sequestration and preservation of CO 2 by soil microorganisms as well as photosynthetic organisms (Bhattacharjee et al, 2023; Macreadie et al, 2014; Moreira et al, 2023). Microbial inoculants might be applied to elevate carbon intake while decreasing carbon release from the terrestrial pool.…”

Section: Carbon Sequestration In Soilsmentioning

confidence: 99%

Harnessing the power of microbes for sustainable development: Climate change mitigation and sustainable food security

Ahmed,

McKay

2023

Ecological Research

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The effects of releasing carbon from the terrestrial pool into the atmosphere have significant long‐term and short‐term implications that affect many parts of our globe. Agricultural fields might help to address these concerns and provide cost‐effective solutions, such as lowering carbon dioxide levels (CO2) in the atmosphere while raising carbon levels in the soil. In order to increase agricultural land's potential to absorb and store carbon, the proper selection of suitable microbial inoculants with the capability to sequester carbon into soils is critical. Soil quality and characteristics would improve as a result, and atmospheric carbon would therefore be reduced. Soil microbes have the potential to influence the level of organic matter, which has an impact on the soil's ecological system and characteristics. Soil microbes play a role in carbon sequestration in soils by regulating multiple and distinct pathways for CO2 inputs and losses, such as biochemical processes that could sequester CO2, the capacity to sediment carbonates, the rigid nature of their components and vegetative tissues, or the composition of complex substances that preserve carbon in the soil. Further research is needed to investigate if particular microbial strains that can sequester carbon will help enhance soil quality and prevent climate change.

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“…Because microbial interactions can benefit plant sustainability and productivity, it is important to understand the genetic and environmental factors that determine interactions and their outcome on plants and their surrounding environments. Understanding the ecological and evolutionary principles governing these interactions provides an opportunity to engineer microbes and plants to achieve more sustainable and productive ecosystems [ 7 ] and mitigate risks associated with introducing microbes into non-native environments [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Lectin Receptor-like Kinase Signaling during Engineered Ectomycorrhiza Colonization

Shrestha

Yao

Qiao

et al. 2023

Cells

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Mutualistic association can improve a plant’s health and productivity. G-type lectin receptor-like kinase (PtLecRLK1) is a susceptibility factor in Populus trichocarpa that permits root colonization by a beneficial fungus, Laccaria bicolor. Engineering PtLecRLK1 also permits L. bicolor root colonization in non-host plants similar to Populus trichocarpa. The intracellular signaling reprogramed by PtLecRLK1 upon recognition of L. bicolor to allow for the development and maintenance of symbiosis is yet to be determined. In this study, phosphoproteomics was utilized to identify phosphorylation-based relevant signaling pathways associated with PtLecRLK1 recognition of L. bicolor in transgenic switchgrass roots. Our finding shows that PtLecRLK1 in transgenic plants modifies the chitin-triggered plant defense and MAPK signaling along with a significant adjustment in phytohormone signaling, ROS balance, endocytosis, cytoskeleton movement, and proteasomal degradation in order to facilitate the establishment and maintenance of L. bicolor colonization. Moreover, protein–protein interaction data implicate a cGMP-dependent protein kinase as a potential substrate of PtLecRLK1.

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Engineering plant microbiomes by integrating eco-evolutionary principles into current strategies

Cited by 32 publications

References 91 publications

Order among chaos: high throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system

Order among chaos: high throughput MYCroplanters can distinguish interacting drivers of host infection in a highly stochastic system

Harnessing the power of microbes for sustainable development: Climate change mitigation and sustainable food security

Lectin Receptor-like Kinase Signaling during Engineered Ectomycorrhiza Colonization

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