Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

Erben, Amelie; Kellerer, Thomas; Lissner, Josefine; Eulenkamp, Constanze; Hellerer, Thomas; Clausen‐Schaumann, Hauke; Sudhop, Stefanie; Heymann, Michaël

doi:10.1101/2022.03.16.484492

Cited by 3 publications

(3 citation statements)

References 85 publications

(121 reference statements)

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“…Detailed descriptions of TD-and FD-FLIM can be found in, 9 while instant FLIM and SUPER-FLIM are based on the design detailed in. 22…”

Section: Fluorescent Beadsmentioning

confidence: 99%

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Rigorous investigation and comparison of different fluorescence lifetime imaging microscopy (FLIM) techniques analyzed using the phasor plot

Kellerer,

Janusch,

Moser

et al. 2024

Multiphoton Microscopy in the Biomedical Sciences XXIV

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In microscopy, quantitative information such as fluorescence lifetime allows researchers to provide detailed information about the microenvironment and the physicochemical state of the molecule under study. However, the high number of influencing factors might be an explanation for the strongly deviating values of fluorescent lifetimes for the same fluorophore as reported in literature. This could be the reason for the impression that inconsistent results are obtained depending on which FLIM technique is used. To clarify this controversy, the two most common as well as two newly developed techniques for measuring fluorescence lifetimes in the time-domain (TD) and in the frequency-domain (FD) were implemented in one single microscopy setup. Furthermore, we applied each of them to a variety of fluorophores under different environmental conditions such as pH-value, temperature, solvent polarity, etc., along with distinct state forms that depend, for example, on the concentration. From a vast amount of measurement results, both setup- and sample-dependent parameters were extracted and represented using a single display form, the phasor-plot. The measurements showed consistent results between the techniques and revealed which of the tested parameters has the strongest influence on the fluorescence lifetime. In addition, together with the instant FLIM setup we present a new technique that we coined Speed-Up PhasE-Resolved (SUPER)-FLIM, which we adapted from elsewhere such that it combines the advantages from TD- with FD-FLIM. For the first time, we demonstrate that this method opens the door for very fast (124ns/px) FLIM measurements required for imaging with a resonant galvo scanner.

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“…Detailed descriptions of TD-and FD-FLIM can be found in, 9 while instant FLIM and SUPER-FLIM are based on the design detailed in. 22…”

Section: Fluorescent Beadsmentioning

confidence: 99%

“…The TD, instant FLIM, and SUPER-FLIM setups (top left, bottom left, and bottom right, respectively) utilize laser scanning modalities, whereas the FD-FLIM system (top right) employs a wide-field approach. Detailed descriptions of TD-and FD-FLIM can be found in,9 while instant FLIM and SUPER-FLIM are based on the design detailed in 22.…”

mentioning

confidence: 99%

Rigorous investigation and comparison of different fluorescence lifetime imaging microscopy (FLIM) techniques analyzed using the phasor plot

Kellerer,

Janusch,

Moser

et al. 2024

Multiphoton Microscopy in the Biomedical Sciences XXIV

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“…[36,37] The algorithmic design framework is not just limited to simple perfusable shapes but can also be expanded to more complex shapes such as alveoli surrounded with a perfusable vessel network. [38] The alveolus is created by dividing a sphere into Rapid design and fabrication of perfusable constructs. A).…”

Section: Algorithmic Design Of Perfusable Architecturesmentioning

confidence: 99%

Synergizing Algorithmic Design, Photoclick Chemistry and Multi‐Material Volumetric Printing for Accelerating Complex Shape Engineering

et al. 2023

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The field of biomedical design and manufacturing has been rapidly evolving, with implants and grafts featuring complex 3D design constraints and materials distributions. By combining a new coding‐based design and modeling approach with high‐throughput volumetric printing, a new approach is demonstrated to transform the way complex shapes are designed and fabricated for biomedical applications. Here, an algorithmic voxel‐based approach is used that can rapidly generate a large design library of porous structures, auxetic meshes and cylinders, or perfusable constructs. By deploying finite cell modeling within the algorithmic design framework, large arrays of selected auxetic designs can be computationally modeled. Finally, the design schemes are used in conjunction with new approaches for multi‐material volumetric printing based on thiol‐ene photoclick chemistry to rapidly fabricate complex heterogeneous shapes. Collectively, the new design, modeling and fabrication techniques can be used toward a wide spectrum of products such as actuators, biomedical implants and grafts, or tissue and disease models.

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mSLAb – An open-source masked stereolithography (mSLA) bioprinter

Kaufmann,

Rudolph,

Pechtl

et al. 2024

HardwareX

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Engineering Principles and Algorithmic Design Synthesis for Ultracompact Bio-Hybrid Perfusion Chip

Cited by 3 publications

References 85 publications

Rigorous investigation and comparison of different fluorescence lifetime imaging microscopy (FLIM) techniques analyzed using the phasor plot

Rigorous investigation and comparison of different fluorescence lifetime imaging microscopy (FLIM) techniques analyzed using the phasor plot

Synergizing Algorithmic Design, Photoclick Chemistry and Multi‐Material Volumetric Printing for Accelerating Complex Shape Engineering

mSLAb – An open-source masked stereolithography (mSLA) bioprinter

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