Investigation into the cause of road failure has been carried out along a 60km Sagamu –Papalanto highway southwestern Nigeria. Geochemical, mineralogical, geotechnical and geophysical analyses were conducted to evaluate the cause of failure along the study area. The results of the laboratory tests showed that the percentage amount of fines ranges from 12-61.3%, natural moisture content from 6.8 to 19.7%, liquid limit in the range of 25.1-52.2%, linear shrinkage between 3.96 to 12.71%, plastic limit ranges from 18.2-35%, plasticity index ranges from 5.2 to 24.6%, free swell in the range from 5.17 – 43.9%, maximum dry density ranges from 1.51 -1.74g /cm3, specific gravity ranges from 2.52-2.64 and CBR between 3-12%. The Cone Penetrometer Test (CPT) shows a resistance value of 20-138 kgf/cm2. The major clay mineral that is predominant in the studied soil is kaolinite. The major oxides present are SiO2, Al2O3, Fe2O3, K2O, Na2O, MgO and CaO. The result of the 2D Electrical Resistivity Imaging revealed a low resistivity values for profile 2 and 3 ranging from 100 Ωm – 300 Ωm, between a distance of 20m – 240 m along the profile to a depth of 7.60m and a low resistivity value ranging from 50 Ωm – 111Ωm, between a distance of 80 m –120 m along the profile to a depth of 15m. It was concluded that the low CBR, low MDD and the class of subsoils namely A-26, A-7, A-2-7 (clayey soils) which were identified are responsible for the cause of failure experienced in the study area. These makes the soils unsuitable as road construction materials and hence, there is need for stabilization during the reconstruction and rehabilitation of the road.