Engineering Ralstonia eutropha for Production of Isobutanol from CO2, H2, and O2

Brigham, Christopher J.; Gai, Claudia S.; Lu, Jingnan; Speth, Daan R.; Worden, R. Mark; Sinskey, Anthony J.

doi:10.1007/978-1-4614-3348-4_39

Cited by 16 publications

(18 citation statements)

References 94 publications

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“…The addition of 5,000 U mL À1 catalase alone without g-C 3 N 4 did not improve PHB production, which demonstrates that R. eutropha cells could not use the enzyme as substrate for their metabolism. (Brigham et al, 2013). (B and C) (B) PHB production from CO 2 over time and (C) PHB concentration after 48 h. REH16, R. eutropha H16.…”

Section: Hybrid Photosynthesis With G-c 3 N 4 -Catalase and R Eutropha For Co 2 Reductionmentioning

confidence: 99%

Nonmetallic Abiotic-Biological Hybrid Photocatalyst for Visible Water Splitting and Carbon Dioxide Reduction

Tremblay

Chen

et al. 2020

iScience

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Both artificial photosystems and natural photosynthesis have not reached their full potential for the sustainable conversion of solar energy into specific chemicals. A promising approach is hybrid photosynthesis combining efficient, non-toxic, and low-cost abiotic photocatalysts capable of water splitting with metabolically versatile non-photosynthetic microbes. Here, we report the development of a water-splitting enzymatic photocatalyst made of graphitic carbon nitride (g-C 3 N 4 ) coupled with H 2 O 2 -degrading catalase and its utilization for hybrid photosynthesis with the nonphotosynthetic bacterium Ralstonia eutropha for bioplastic production. The g-C 3 N 4 -catalase system has an excellent solar-to-hydrogen efficiency of 3.4% with a H 2 evolution rate up to 55.72 mmol h À1 while evolving O 2 stoichiometrically. The hybrid photosynthesis system built with the water-spitting g-C 3 N 4 -catalase photocatalyst doubles the production of the bioplastic polyhydroxybutyrate by R. eutropha from CO 2 and increases it by 1.84-fold from fructose. These results illustrate how synergy between abiotic non-metallic photocatalyst, enzyme, and bacteria can augment solar-to-multicarbon chemical conversion.

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Section: Hybrid Photosynthesis With G-c 3 N 4 -Catalase and R Eutropha For Co 2 Reductionmentioning

confidence: 99%

Nonmetallic Abiotic-Biological Hybrid Photocatalyst for Visible Water Splitting and Carbon Dioxide Reduction

Tremblay

Chen

et al. 2020

iScience

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“…In this model, the organism uses its membrane bound NiFe-hydrogenase (MBH) to supply electrons to its electron transfer chain for ATP generation. The soluble hydrogenase (SBH) is used for the direct reduction of NAD + , which is then used to generate NADPH via a transhydrogenase reaction 89 . This NADPH is used for subsequent biosynthetic reactions, including carbon fixation.…”

Section: Bringing Electrons To the Cell Surfacementioning

confidence: 99%

“…eutropha is typically grown under an atmosphere containing H2, O2 and CO2 at a ratio of 8:1:1 89 . At the laboratory-scale, the H2 pressure in the headspace is typically restricted to 5% or less of a total of 1 atmosphere in order to reduce the risks of H2 explosion 89 . Under these conditions a 1 L volume is restricted to a geometry of, ,…”

Section: Bringing Electrons To the Cell Surfacementioning

confidence: 99%

Section: Bringing Electrons To the Cell Surfacementioning

confidence: 99%

“…A high H2 atmosphere is maintained on the exterior of the fiber while a high O2 atmosphere is maintained on the interior. This permits packaging of high surface areas within a reasonable volume while affording the culture access to both gases but minimizes mixing and thus circumvents some of the safety concerns of containing both within the same vessel 89 . The recycled-gas culture system designed by Schlegel and Lafferty [95][96][97] which increases H2 transfer rate by stirring and uses and elaborate H2 storage system to minimize gas losses.…”

Section: Bringing Electrons To the Cell Surfacementioning

confidence: 99%

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Molecular Mechanisms for the Biological Storage of Renewable Energy

Barstow

2015

Preprint

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Recent and ongoing discoveries in the field of extracellular electron transport offer the potential to electrically power highly flexible, carbon-fixing microbial metabolisms and generate a rich variety of chemicals and fuels. This process, electrosynthesis, creates the opportunity to use biology for the low cost storage of renewable electricity and the synthesis of fuels that produce no net carbon dioxide. This article highlights recent discoveries on the molecular machinery underpinning electrosynthesis and reviews recent work on the energy conversion efficiency of photosynthesis to begin to establish a framework to quantify the overall energy storage and conversion efficiency of electrosynthesis.

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