Engineering Resistance in Brinjal against Nematode (Meloidogyne Incognita) Using cry1Ab Gene from Bacillus Thuringiensis Berliner

Abstract

Transgenic brinjal plants (Solanum melongena cv. Co2) expressing cryIAb gene from Bacillus thuringiensis Berliner (Bt) were evolved by Agrobacterium-mediated transformation system. In the present study, 7 T 0 lines (P-Co2IAb-5, P-Co2IAb-9, P-Co2IAb-10, P-Co2IAb-12, P-Co2IAb-16, P-Co2IAb-17 and P-Co2IAb-22) harboring cryIAb gene were obtained. PCR and Southern blotting analyses proved the presence and integration of cryIAb in these lines. A polyclonal rabbit anti-CryIAb antiserum detected the expression of a 65 kDa CryIAb polypeptide in all these lines. Expression of CryIAb protein in those transgenic lines was also confirmed by lateral flow sticks (CryIAb) assay. Molecular analyses in T 1 progenies harboring cryIAb gene proved the presence and expression of the transgene of interest. The chi-square (χ 2 ) tests revealed a 3:1 segregation ratio for cryIAb gene in the lines viz., P-Co2IAb-5 and P-Co2IAb-9, indicating that the transgene was integrated at a single locus.The transgenic lines thus obtained in this study were used to investigate the potential of the CryIAb protein in controlling the root knot nematode, Meloidogyne incognita. Bioassay for root knot nematode resistance in T 1 plants expressing CryIAb showed that there was a significant decrease in number of galls and eggmasses in cryIAb-transgenic lines (P-Co2IAb-5 and P-Co2IAb-9) as compared to control. In addition, there was a significant decrease in the number of eggs per eggmass on those transgenic lines tested than the non-transgenic controls. Thus, transgenic Bt-brinjal plants could also form a part of an integrated nematode management program.

Responses of nematode‐community structure in turfgrass soil to microbial filtrates from municipal–solid waste compost

“…Neidig et al (2011) found that bacterial secondary metabolites responsible for the suppression of plant pathogens strongly inhibited bacterivorous nematodes and thus likely contributed to the resistance of bacteria against predators in soil. Phap et al (2010) investigated the potential of CryIAb gene from B. thuringiensis Berliner in controlling the root-knot nematode, Meloido-gyne incognita. Xu et al (2010) Municipal-solid waste (MSW) composting has proved to be a safe and effective way to make full use of MSW resources and to divert organic-waste materials to soil amendment or fertilizer (Shiralipour et al, 1992;Moldes et al, 2007;Herrera et al, 2008).…”

Reproduction of root knot nematode (</i>Meloidogyne incognita<i>) on Bt cotton expressing Cry1Ac and Cry2Ab2 protein

“…M. incognita ingested the toxic 54 KDA protein which resulted in a fourfold decrease in reproduction. Phap et al (2010) reported that Cry1Ab protein in transgenic brinjal resulted in a decrease in the number of galls, egg masses and number of eggs per egg mass. Bt cotton has been evaluated for its reaction to parasitic nematodes.…”