Engineering Rhodosporidium toruloides for sustainable production of value-added punicic acid from glucose and wood residues

Wang, Juli; Haddis, Dagem Z.; Xiao, Qiong; Bressler, David C.; Chen, Guanqun

doi:10.1016/j.biortech.2024.131422

Bioresource Technology

2024

DOI: 10.1016/j.biortech.2024.131422

|View full text |Cite

Engineering Rhodosporidium toruloides for sustainable production of value-added punicic acid from glucose and wood residues

Juli Wang,

Dagem Z. Haddis,

Qiong Xiao

et al.

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article1

Relationship

Self Cite0

Independent1

Authors

Journals

Cited by 1 publication

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Engineering Saccharomyces cerevisiae for the Production of Punicic Acid-Rich Yeast Biomass

Wang,

Chen

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Punicic acid (PuA), an unusual conjugated linolenic acid found in pomegranate, offers diverse health benefits and has potential applications in the food industry. Due to the limited availability of PuA from natural plant sources, there is growing interest in producing it through microbial fermentation. In this study, the yeast Saccharomyces cerevisiae, which is classified as "generally recognized as safe", was engineered to produce PuA using a results-driven approach. Genes potentially involved in PuA synthesis were integrated directly into the yeast genome, targeting Ty retrotransposon sites. Screening of the yeast transformants, followed by optimization of culture conditions, resulted in the production of 26.7% PuA within the yeast's total fatty acids. Further analysis revealed that the strain's triacylglycerol fraction contained over 22% PuA. By incorporating this health-promoting lipid into the nutritional profile of S. cerevisiae, the engineered strain could serve as a sustainable source of yeast biomass with enhanced nutritional value.

show abstract

Engineering Saccharomyces cerevisiae for the Production of Punicic Acid-Rich Yeast Biomass

Wang,

Chen

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Engineering Rhodosporidium toruloides for sustainable production of value-added punicic acid from glucose and wood residues

Cited by 1 publication

References 47 publications

Engineering Saccharomyces cerevisiae for the Production of Punicic Acid-Rich Yeast Biomass

Engineering Saccharomyces cerevisiae for the Production of Punicic Acid-Rich Yeast Biomass

Contact Info

Product

Resources

About