Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin

Ren, Yaoyao; Qin, Zongmin; Li, Congcong; Yuan, Bo; Yang, Yang; Qu, Ge; Sun, Zhoutong

doi:10.1016/j.jbiotec.2024.03.004

Journal of Biotechnology

2024

DOI: 10.1016/j.jbiotec.2024.03.004

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Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin

Yaoyao Ren,

Zongmin Qin,

Congcong Li

et al.

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Cited by 2 publications

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Biocatalytic synthesis of vanillin from biomass-derived compounds: A review

Zhang,

Jiang,

Shen

et al. 2025

Catalysis Today

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Biocatalytic synthesis of vanillin from biomass-derived compounds: A review

Zhang,

Jiang,

Shen

et al. 2025

Catalysis Today

View full text Add to dashboard Cite

Computation-driven redesign of an NRPS-like carboxylic acid reductase improves activity and selectivity

Shi,

Li,

Wang

et al. 2024

Sci. Adv.

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Engineering nonribosomal peptide synthetases (NRPSs) has been a “holy grail” in synthetic biology due to their modular nature and limited understanding of catalytic mechanisms. Here, we reported a computational redesign of the “gate-keeper” adenylation domain of the model NRPS-like enzyme carboxylic acid reductases (CARs) by using approximate mechanism-based geometric criteria and the Rosetta energy score. Notably, Mab CAR3 mutants ACA-1 and ACA-4 displayed a remarkable improvement in catalytic efficiency ( k cat / K M ) for 6-aminocaproic acid, up to 101-fold. Furthermore, G418K exhibited an 86-fold enhancement in substrate specificity for adipic acid compared to 6-aminocaproic acid. Our work provides not only promising biocatalysts for nylon monomer biosynthesis but also a strategy for efficient NRPSs engineering.

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Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin

Cited by 2 publications

References 52 publications

Biocatalytic synthesis of vanillin from biomass-derived compounds: A review

Biocatalytic synthesis of vanillin from biomass-derived compounds: A review

Computation-driven redesign of an NRPS-like carboxylic acid reductase improves activity and selectivity

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