Engineering the cellular mechanical microenvironment to regulate stem cell chondrogenesis: Insights from a microgel model

Feng, Qi; Gao, Huichang; Wen, Hongji; Huang, Hanhao; Li, Qingtao; Liang, Minhua; Liu, Yang; Dong, Hua; Cao, Xiaodong

doi:10.1016/j.actbio.2020.06.046

Cited by 49 publications

(45 citation statements)

References 90 publications

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“…In spite of the capability of hydrogels to mimic the extracellular matrix (ECM), their large size (i.e., low surface to volume ratio resulting in small diffusion area and long diffusion distance for soluble molecules) could hinder the uniform distribution of biophysical cues/nutrients leading to a biochemical gradient within the microgels and thus impeding high-throughput screening and evaluation. In this respect, Feng and co-workers [ 87 ] proposed a microgel model (i.e., gelatin/HA microgels formed in microfluidic devices and exhibiting a low, medium and high degree of cross-linking) ( Figure 9 ) mimicking the ECM microenvironment to examine in vitro the effect of mechanical cues embedded in the cellular microenvironment on the fate of bone marrow derived MSCs (BMSCs). BMSCs cultured in hydrogel beads of low cross-linking density were shown to differentiate to hyaline cartilage as opposed to those cultured in microgels with medium and high cross-linking density, which differentiated towards fibrocartilage, thus verifying the effect of the mechanical microenvironment on the proliferation, distribution and differentiation of MSCs.…”

Section: Hydrogels—preclinical Evaluationmentioning

confidence: 99%

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Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

2022

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Articular cartilage lesions resulting from injurious impact, recurring loading, joint malalignment, etc., are very common and encompass the risk of evolving to serious cartilage diseases such as osteoarthritis. To date, cartilage injuries are typically treated via operative procedures such as autologous chondrocyte implantation (ACI), matrix-associated autologous chondrocyte implantation (MACI) and microfracture, which are characterized by low patient compliance. Accordingly, cartilage tissue engineering (CTE) has received a lot of interest. Cell-laden hydrogels are favorable candidates for cartilage repair since they resemble the native tissue environment and promote the formation of extracellular matrix. Various types of hydrogels have been developed so far for CTE applications based on both natural and synthetic biomaterials. Among these materials, hyaluronic acid (HA), a principal component of the cartilage tissue which can be easily modified and biofunctionalized, has been favored for the development of hydrogels since it interacts with cell surface receptors, supports the growth of chondrocytes and promotes the differentiation of mesenchymal stem cells to chondrocytes. The present work reviews the various types of HA-based hydrogels (e.g., in situ forming hydrogels, cryogels, microgels and three-dimensional (3D)-bioprinted hydrogel constructs) that have been used for cartilage repair, specially focusing on the results of their preclinical and clinical assessment.

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Section: Hydrogels—preclinical Evaluationmentioning

confidence: 99%

“… Microfluidic fabrication and characterization of BMSC-laden gel-HA microgels: ( a ) effects of flow rate ratios of oil/water on the diameter of BMSC-laden gel-HA microgels; ( b ) BMSC viability and proliferation behaviors in gel-HA microgels (reprinted with the permission from [ 87 ]). …”

Section: Figurementioning

confidence: 99%

Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

2022

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“…Wongpinyochit et al [95] injected SF solution and organic solvent into two separate microfluidic channels, and then these two phases met and mixed at the junction, inducing the selfassembly of SF spheres. Microfluidic mixing was proved to not only allow the sphere size to be tuned by varying the flow Gelatin [116] Microsphere Physical crosslinking --Gelatin [117] Microsphere Enzymatic crosslinking --Gel-SH [119] Microsphere Chemical crosslinking BMSCs Cell BP-grafted GelMA [121] Microsphere Photo-crosslinking --…”

Section: Sfmentioning

confidence: 99%

“…On the other hand, the amino and carbonyl groups of gelation make it easy to have chemical modification. For example, thiol-modified gelatin (Gel-SH) was synthesized and could be rapidly crosslinked with HA-VS via thiol-Michael addition reaction at room temperature [119]. Using Gel-SH and HA-VS as dispersed phases, respectively, Gel-SH microspheres had spontaneous crosslinking once the liquid phases met without additional external stimulation.…”

Section: Gelationmentioning

confidence: 99%

Biomaterials for microfluidic technology

Chen

Zhang

et al. 2022

Mater. Futures

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Micro/nanomaterial-based drug and cell delivery systems play an important role in biomedical fields for their injectability and targeting. Microfluidics is a rapidly developing technology and has become a robust tool for preparing biomaterial micro/nanocarriers with precise structural control and high reproducibility. By flexibly designing microfluidic channels and manipulating fluid behavior, various forms of biomaterial carriers can be fabricated using microfluidics, including microspheres, nanoparticles and microfibers. In this review, recent advances in biomaterials for designing functional microfluidic vehicles are summarized. We introduce the application of natural materials such as polysaccharides and proteins as well as synthetic polymers in the production of microfluidic carriers. How the material properties determine the manufacture of carriers and the type of cargoes to be encapsulated is highlighted. Furthermore, the current limitations of microfluidic biomaterial carriers and perspectives on its future developments is presented.

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“…Besides, such bulk hydrogels have merely nanoporous meshes inside the crosslinked network and lack of micropores, indicative of insufficient nutrient exchange and low cell viability inside hydrogels [ 8 , 9 ]. A promising solution to this problem is the replacement of bulk hydrogel with microporous microgel assembly (also called as microporous annealed particle, MAP), whose large surface/volume ratio and short diffusion distance enhance the mass transport of nutrients and thus promote the long-term survival of cells [ [10] , [11] , [12] ]. Moreover, its interconnected pores can also guide cell ingrowth and tissue formation before hydrogel degradation.…”

Section: Introductionmentioning

confidence: 99%

Microgel assembly: Fabrication, characteristics and application in tissue engineering and regenerative medicine

Feng

et al. 2022

Bioactive Materials

Self Cite

109

106

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Microgel assembly, a macroscopic aggregate formed by bottom-up assembly of microgels, is now emerging as prospective biomaterials for applications in tissue engineering and regenerative medicine (TERM). This mini-review first summarizes the fabrication strategies available for microgel assembly, including chemical reaction, physical reaction, cell-cell interaction and external driving force, then highlights its unique characteristics, such as microporosity, injectability and heterogeneity, and finally itemizes its applications in the fields of cell culture, tissue regeneration and biofabrication, especially 3D printing. The problems to be addressed for further applications of microgel assembly are also discussed.

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Engineering the cellular mechanical microenvironment to regulate stem cell chondrogenesis: Insights from a microgel model

Cited by 49 publications

References 90 publications

Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

Recent Developments in Hyaluronic Acid-Based Hydrogels for Cartilage Tissue Engineering Applications

Biomaterials for microfluidic technology

Microgel assembly: Fabrication, characteristics and application in tissue engineering and regenerative medicine

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