Engineering the Extracellular Matrix for Organoid Culture

Heo, Jeong Hyun; Kang, Dongyun; Seo, Seung Ju; Jin, Yoonhee

doi:10.15283/ijsc21190

Cited by 49 publications

(39 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…OptoGels are promising tools for scaling clinically viable cell cultures and organoids, which are miniature in vitro tissue approximations for in vivo organs ( Kim et al (2020) ; Heo et al (2022) ). While nearly all cells of our bodies develop in a 3D environment, multiple cell culture techniques are still 2D, likely due to the ease of passaging and imaging monolayers.…”

Section: Emerging Applicationsmentioning

confidence: 99%

“…The gold standard scaffold for generating organoids is Matrigel, a passive gel whose properties can be neither spatially nor dynamically controlled ( Heo et al (2022) ). Thus, organoid model systems can suffer from a lack of reproducibility ( Gjorevski et al (2022) ), which is especially problematic in clinical adaptions where reproducible organoid patterning will be essential.…”

Section: Emerging Applicationsmentioning

confidence: 99%

See 1 more Smart Citation

Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science

Månsson

Pitenis

Wilson

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

We review fundamental mechanisms and applications of OptoGels: hydrogels with light-programmable properties endowed by photoswitchable proteins (“optoproteins”) found in nature. Light, as the primary source of energy on earth, has driven evolution to develop highly-tuned functionalities, such as phototropism and circadian entrainment. These functions are mediated through a growing family of optoproteins that respond to the entire visible spectrum ranging from ultraviolet to infrared by changing their structure to transmit signals inside of cells. In a recent series of articles, engineers and biochemists have incorporated optoproteins into a variety of extracellular systems, endowing them with photocontrollability. While other routes exist for dynamically controlling material properties, light-sensitive proteins have several distinct advantages, including precise spatiotemporal control, reversibility, substrate selectivity, as well as biodegradability and biocompatibility. Available conjugation chemistries endow OptoGels with a combinatorially large design space determined by the set of optoproteins and polymer networks. These combinations result in a variety of tunable material properties. Despite their potential, relatively little of the OptoGel design space has been explored. Here, we aim to summarize innovations in this emerging field and highlight potential future applications of these next generation materials. OptoGels show great promise in applications ranging from mechanobiology, to 3D cell and organoid engineering, and programmable cell eluting materials.

show abstract

Section: Emerging Applicationsmentioning

confidence: 99%

Section: Emerging Applicationsmentioning

confidence: 99%

Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science

Månsson

Pitenis

Wilson

2022

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…Recently, several efforts were developed to find synthetic alternatives for the Matrigel [51], [53], [54]. These alternative materials may provide a chemically defined and xeno-free culture niche that can be independently tuned by adjusting various variables, such as its composition and the polymerization method to produce the expected mechanical and biological properties [51], [53]. Moreover, by using a bioengineering approach, these synthetic matrices can be further utilized to provide a specific boundary guiding tissue morphogenesis [55].…”

Section: Matrigel Embedded Techniquementioning

confidence: 99%

Strategies for Generating Human Pluripotent Stem Cell- Derived-Organoid Culture for Disease Modelling, Drug Screening, and Regenerative Therapy

Gania¹,

Noorintan²,

Septiari³

et al. 2022

Preprint

View full text Add to dashboard Cite

Human pluripotent stem cells (hPSCs) have become a powerful tool to generate various kinds of cell types comprising the human body. Recently, organoid technology emerged as a platform to build a physiologically relevant tissue-like structure from the PSCs, which provides a more relevant three-dimensional microenvironment to the actual human body than the conventional monolayer culture system for transplantation, disease modeling, and drug development. Although it holds so many advantages, the organoid culture system still has various problems related to culture methods, which became a challenge to get similar physiological properties to their original tissue counterparts. Here, we discuss the current development of organoid culture methods, including the problem that may arise from the currently available culture systems as well as the possible approach to overcoming the current limitation and improving their optimum utilization for translational application purposes.

show abstract

“…Micromachining, 3D printing, and hydrogels are the basic elements of 3D cell culture techniques [ 1 ]. Various 3D culture methods for different tissues have been developed in recent years, which laid a solid foundation for the development of organoid culture techniques [ 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ]. Stem-cell-derived organoids are in vitro organ models that form functional tissue types by self-organization and differentiation, which can potentially facilitate a better understanding of the biology and physiology of various organs [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review

Yue

Liu

et al. 2022

Gels

View full text Add to dashboard Cite

Organoids are novel in vitro cell culture models that enable stem cells (including pluripotent stem cells and adult stem cells) to grow and undergo self-organization within a three-dimensional microenvironment during the process of differentiation into target tissues. Such miniature structures not only recapitulate the histological and genetic characteristics of organs in vivo, but also form tissues with the capacity for self-renewal and further differentiation. Recent advances in biomaterial technology, particularly hydrogels, have provided opportunities to improve organoid cultures; by closely integrating the mechanical and chemical properties of the extracellular matrix microenvironment, with novel synthetic materials and stem cell biology. This systematic review critically examines recent advances in various strategies and techniques utilized for stem-cell-derived organoid culture, with particular emphasis on the application potential of hydrogel technology in organoid culture. We hope this will give a better understanding of organoid cultures for modelling diseases and tissue engineering applications.

show abstract

Engineering the Extracellular Matrix for Organoid Culture

Cited by 49 publications

References 52 publications

Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science

Extracellular Optogenetics at the Interface of Synthetic Biology and Materials Science

Strategies for Generating Human Pluripotent Stem Cell- Derived-Organoid Culture for Disease Modelling, Drug Screening, and Regenerative Therapy

Advances of Engineered Hydrogel Organoids within the Stem Cell Field: A Systematic Review

Contact Info

Product

Resources

About