Engineering the filamentous fungus Penicillium oxalicum for rapid, low-background and efficient protein expression

Xiang, Boyu; Zhao, Shengfang; Chen, Jie; Chen, Yu; Zhu, Cui; Hu, Shengbiao; Hu, Yibo

doi:10.1016/j.enzmictec.2022.110150

Cited by 4 publications

(1 citation statement)

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“…Another drawback of some filamentous fungal strains is that the secretion of recombinant proteins is accompanied by the secretion of host proteins, which in many cases also include proteases, and this could (1) affect the subsequent purification steps and application of the target protein(s), and (2) result in degradation of the heterologous protein(s). The construction of low background host strains in filamentous fungi has been implemented [ 13 , 14 ]. However, the application of mutagenesis to obtain hosts with low background expression is labor intensive.…”

Section: Introductionmentioning

confidence: 99%

Strategies for the Development of Industrial Fungal Producing Strains

Salazar-Cerezo

Vries

Garrigues

2023

JoF

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The use of microorganisms in industry has enabled the (over)production of various compounds (e.g., primary and secondary metabolites, proteins and enzymes) that are relevant for the production of antibiotics, food, beverages, cosmetics, chemicals and biofuels, among others. Industrial strains are commonly obtained by conventional (non-GMO) strain improvement strategies and random screening and selection. However, recombinant DNA technology has made it possible to improve microbial strains by adding, deleting or modifying specific genes. Techniques such as genetic engineering and genome editing are contributing to the development of industrial production strains. Nevertheless, there is still significant room for further strain improvement. In this review, we will focus on classical and recent methods, tools and technologies used for the development of fungal production strains with the potential to be applied at an industrial scale. Additionally, the use of functional genomics, transcriptomics, proteomics and metabolomics together with the implementation of genetic manipulation techniques and expression tools will be discussed.

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Section: Introductionmentioning

confidence: 99%