Engineering the interface between electroactive bacteria and electrodes

Catania, Chelsea; Karbelkar, Amruta A.; Furst, Ariel L.

doi:10.1016/j.joule.2021.02.001

Cited by 45 publications

(25 citation statements)

References 15 publications

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“…Prior efforts to direct electroactive biofilm formation on surfaces have focused solely on engineering cell-electrode attachment, rather than patterning, through synthetic biology and materials engineering strategies. These works were based on either (I) enhancing biofilm formation by expressing adhesive appendages on cell surfaces 49,50 and increasing c-di-GMP levels 51,52 or (II) placing complementary chemical or DNA-based structures on substrates and cell surfaces to bond cells to electrodes [53][54][55][56][57] . Compared with these previous works, our strategy does not require electrode pretreatment for electroactive biofilm patterning and can generate robust biofilms with defined dimensions.…”

Section: Discussionmentioning

confidence: 99%

Light-induced Patterning of Electroactive Bacterial Biofilms

Zhao

Chavez

Naughton

et al. 2021

Preprint

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Electroactive bacterial biofilms can function as living biomaterials that merge the functionality of living cells with electronic components. However, the development of such advanced living electronics has been challenged by the inability to control the geometry of electroactive biofilms relative to solid-state electrodes. Here, we developed a lithographic strategy to pattern conductive biofilms of Shewanella oneidensis by controlling aggregation protein CdrAB expression with a blue light-induced genetic circuit. This controlled deposition enabled S. oneidensis biofilm patterning on transparent electrode surfaces and measurements demonstrated tunable biofilm conduction dependent on pattern size. Controlling biofilm geometry also enabled us, for the first time, to quantify the intrinsic conductivity of living S. oneidensis biofilms and experimentally confirm predictions based on simulations of a recently proposed collision-exchange electron transport mechanism. Overall, we developed a facile technique for controlling electroactive biofilm formation on electrodes, with implications for both studying and harnessing bioelectronics.

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Section: Discussionmentioning

confidence: 99%

Light-induced Patterning of Electroactive Bacterial Biofilms

Zhao

Chavez

Naughton

et al. 2021

Preprint

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“…As the components of these systems become ever more complex and advanced, this interface becomes increasingly important. 9 Moreover, one consistent challenge in biosensing is the development of systems that can monitor biological signals without perturbing or changing the system of interest, which can cause artifacts in sensor signals and prevent the production of a biologically relevant readout. Thus, there is increasing interest in bioelectrochemical platforms based on functional assays and the behavior and activity of biomolecules rather than simply monitoring passive binding interactions.…”

Section: Functional Assays At the Abiotic–biotic Interfacementioning

confidence: 99%

“… 8 These platforms are also compatible with multiplexing, miniaturization, and automation. 9 Thus, electrochemical biosensors are highly useful for the study of host–pathogen interactions, as well as point-of-care detection of these pathogens. A number of recent reviews, most notably that of Ruiz de Eguilaz et al and Bukkitgar et al, have cataloged the advances in the methods and materials used for these sensors.…”

Section: Introductionmentioning

confidence: 99%

Bioelectrochemical platforms to study and detect emerging pathogens

et al. 2021

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Graphic Abstract The ongoing SARS-CoV-2 pandemic has emphasized the importance of technologies to rapidly detect emerging pathogens and understand their interactions with hosts. Platforms based on the combination of biological recognition and electrochemical signal transduction, generally termed bioelectrochemical platforms, offer unique opportunities to both sense and study pathogens. Improved bio-based materials have enabled enhanced control over the biotic–abiotic interface in these systems. These improvements have generated platforms with the capability to elucidate biological function rather than simply detect targets. This advantage is a key feature of recent bioelectrochemical platforms applied to infectious disease. Here, we describe developments in materials for bioelectrochemical platforms to study and detect emerging pathogens. The incorporation of host membrane material into electrochemical devices has provided unparalleled insights into the interaction between viruses and host cells, and new capture methods have enabled the specific detection of bacterial pathogens, such as those that cause secondary infections with SARS-CoV-2. As these devices continue to improve through the merging of hi-tech materials and biomaterials, the scalability and commercial viability of these devices will similarly improve.

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“…Electrogenic bacteria are a special group of microbes that can release their metabolically generated electrons extracellularly across the cell membrane to terminal acceptors, or acquire electrons from external donors. [1][2][3][4] The bacterial electron transfer to external acceptors is a remarkable and crucial process for their growth, respiration, communication, sensing, and cooperation with surrounding environments (Figure 1). [5,6] On the other hand, exogenous electrons can be transferred into intracellular compartments of bacterial cells, small MFC units has been proposed as an alternative solution for large-scale development, the performance has not noticeably improved because of intrinsic voltage reversal and mass transport limitations within the serially connected units.…”

Section: Introductionmentioning

confidence: 99%

“…[7][8][9] The bidirectional electron transfer occurs via multiple adaptive routes such as direct electron transfer, nanowire transfer, and shuttle transfer, indicating that the electron transfer efficiency is the key factor affecting the microbial electrochemical activities. [2,5,10] With the discovery that external electrodes can effectively serve as electron acceptors or donors, the intensive exploration of bidirectional electron exchange between the bacteria and the electrodes has created novel techniques in various bioelectrochemical systems (e.g., microbial fuel cells (MFCs), microbial electrolysis cells (MECs), microbial desalination cells (MDCs), and microbial electrosynthesis (MES)). [1,11] With the bioelectrochemical systems, the electrogenic bacteria can revolutionarily generate renewable bioelectricity from organic waste, synthesize high-value chemicals and biofuels, or perform many other environmentally important functions, such as bioremediation, desalination, and biosensing.…”

mentioning

confidence: 99%

Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing

Choi

2022

Small

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Considerable research efforts into the promises of electrogenic bacteria and the commercial opportunities they present are attempting to identify potential feasible applications. Metabolic electrons from the bacteria enable electricity generation sufficient to power portable or small‐scale applications, while the quantifiable electric signal in a miniaturized device platform can be sensitive enough to monitor and respond to changes in environmental conditions. Nanomaterials produced by the electrogenic bacteria can offer an innovative bottom‐up biosynthetic approach to synergize bacterial electron transfer and create an effective coupling at the cell–electrode interface. Furthermore, electrogenic bacteria can revolutionize the field of bioelectronics by effectively interfacing electronics with microbes through extracellular electron transfer. Here, these new directions for the electrogenic bacteria and their recent integration with micro‐ and nanosystems are comprehensively discussed with specific attention toward distinct applications in the field of powering, sensing, and synthesizing. Furthermore, challenges of individual applications and strategies toward potential solutions are provided to offer valuable guidelines for practical implementation. Finally, the perspective and view on how the use of electrogenic bacteria can hold immeasurable promise for the development of future electronics and their applications are presented.

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Engineering the interface between electroactive bacteria and electrodes

Cited by 45 publications

References 15 publications

Light-induced Patterning of Electroactive Bacterial Biofilms

Light-induced Patterning of Electroactive Bacterial Biofilms

Bioelectrochemical platforms to study and detect emerging pathogens

Electrogenic Bacteria Promise New Opportunities for Powering, Sensing, and Synthesizing

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