Engineering Thermostable Microbial Xylanases Toward its Industrial Applications

Kumar, Vishal; Dangi, Arun Kumar; Shukla, Pratyoosh

doi:10.1007/s12033-018-0059-6

Cited by 120 publications

(53 citation statements)

References 88 publications

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“…The xylanolytic enzyme system comprises three synergistic biological enzymes, namely, endo--1,4-xylanase, exo--1,4-xylanase, and -xylosidase [7], which are crucial enzymes in the hydrolysis of the -1,4-xylosidic bond of the xylan polymer backbone. Xylanase has been used in various industrial processes, including the pulp and paper industry, textile processing, biofuel industry, organic waste treatment, and the food and feed industries [8]. In the treatment of lignocellulosic biomasses, xylanase is able not only to catalyze the hydrolysis of hemicellulose but also to remove an amount of lignin by degradation of the lignin-carbohydrate complex (LCC) [9].…”

Section: Introductionmentioning

confidence: 99%

Effects of Xylanase Pretreatment on the Quality of Refiner Mechanical Mulberry Branch Fibers

Tao

Guo

Meng

et al. 2019

Advances in Polymer Technology

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We performed xylanase pretreatment prior to mechanical refining in the production of mulberry branch fibers, with the objective of saving energy and studying the effects of such pretreatment on the quality of the fibers. To determine the effects of the enzyme action, we analyzed the energy required for refining, related yield, and the dimension, deformation, and morphology of the fibers. We found that, with the xylanase pretreatment, the refining energy was reduced by 4%, with the yield of fibers being maintained at >85%. In addition, the fiber bundles were defibered further, resulting in reduced average length of the fiber. Furthermore, the fiber widths increased because of the improved swelling effect of the xylanase pretreatment. However, in some instances, the fine elements were reduced. With a low enzyme dosage, the fiber coarseness decreased remarkably and, because of the swelling and softening effects of the xylanase pretreatment on the mulberry branches, the fiber kink ratios and curl were reduced. Additionally, the mulberry branch tissue was loosened, facilitating fiber separation. In view of these findings, the biomechanical process could be a potentially green and efficient process for the manufacturing of mulberry branch fibers.

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Section: Introductionmentioning

confidence: 99%

Effects of Xylanase Pretreatment on the Quality of Refiner Mechanical Mulberry Branch Fibers

Tao

Guo

Meng

et al. 2019

Advances in Polymer Technology

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“…Xylanases have attracted attention due to their potential applications in various industries, including paper, textiles, and food (Kumar, Dangi, & Shukla, 2018). The interest in xylanase and its application in the paper industry has increased over the years (Thomas, Raveendran, Parameswaran, & Pandey, 2015).…”

Section: Introductionmentioning

confidence: 99%

Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching

Rodrigues

Silva

et al. 2019

Acta Sci. Biol. Sci.

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In this study, two hundred fifty-seven bacterial isolates from a suppressive soil library were screened to study their secretion of alkali-thermostable xylanases for potential use in cellulose pulp biobleaching. Xylanase activity was evaluated in solid and liquid media using xylan as the carbon source. Isolates were initially evaluated for the degradation of xylan in solid media by the congo red test. Selected strains were evaluated in liquid media for enzymatic activity and determination of total protein concentration using a crude protein extract (CPE). An isolate identified as Bacillus species TC-DT13 produced the highest amount of xylanase (1808 U mL-1). The isolate was active and stable at 70°C and pH 9.0, conditions which are necessary for the paper industry. This isolate can grow and produce xylanase in medium containing wheat fiber as a substrate. The CPE of this isolate was used in preliminary testing on cellulose pulp bleaching; enzyme treatment of the pulp resulted in a 5% increase of whiteness.

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“…Xylan consists of xylose units linked by β-1,4 glycosyl linkages (Singh et al 2013). Complete degradation of xylan requires synergistic hydrolytic enzymes, including enzymes responsible for the degradation of xylan backbone and its various side chain groups (Kumar et al 2018). Xylanases catalyze the hydrolysis of xylan by cleaving the β-1,4-xylosidic bonds in the backbone of xylan, which consists of xylose units (Kumar et al 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Complete degradation of xylan requires synergistic hydrolytic enzymes, including enzymes responsible for the degradation of xylan backbone and its various side chain groups (Kumar et al 2018). Xylanases catalyze the hydrolysis of xylan by cleaving the β-1,4-xylosidic bonds in the backbone of xylan, which consists of xylose units (Kumar et al 2018). Endo-xylanases (EC 3.2.1.8) and βxylosidases (EC 3.2.1.37) are the major enzymes involved in xylan degradation (Juturu and Wu 2012).…”

Section: Introductionmentioning

confidence: 99%

“…bleaching in the paper industry, liquefaction of coffee mucilage, maceration of vegetable, extraction of pigments and flavors, bioremediation of agricultural wastes (Kulkarni et al 1999), and saccharification of xylan (Juturu and Wu 2012). Most of these industrial processes require stable xylanases at high temperatures (Kumar et al 2018); for example, the conversion of xylan into xylose during saccharification process occurs at 40−80°C (Juturu and Wu 2012). Therefore, the findings of microorganisms that are able to produce stable and active xylanases at high temperatures will have a great impact on various industrial applications (Beg et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

Rachmania¹,

Ningsih²,

Setyaningsih³

et al. 2019

Biodiversitas

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Abstract. Rachmania MK, Ningsih F, Setyaningsih PP, Syafitri WA, Sari DCAF, Yabe S, Yokota A, Oetari A, Sjamsuridjal W. 2020. Xylan-degrading ability of thermophilic Actinobacteria isolated from soil in a geothermal area. Biodiversitas 21: 144-154. This study was conducted to obtain the potential isolates of xylan-degrading thermophilic Actinobacteria. Seventeen isolates were obtained from the soil samples collected from Cisolok geysers in West Java, Indonesia. These isolates were screened for their xylan-degrading ability on minimal medium with the addition of 0.5% xylan as substrate, and were incubated at various temperatures for 7 days. A total of 15, 14, 4, and 3 isolates showed a xylan-degrading ability at 45°C, 50°C, 55°C, and 60°C, respectively. The three isolates (SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4) that showed xylan-degrading ability at 60°C on 0.5% xylan were further tested by using minimal medium with the addition of 0.1% RBB-xylan as a substrate. The results showed that these isolates were also able to utilized RBB-xylan at 45 to 60°C. The xylanolytic activity of the crude enzymes from the three isolates on minimal medium containing 0.5% xylan or 0.1% RBB-xylan as substrates, indicated that these isolates were able to produce extracellular xylanase. This study revealed that the soil of Cisolok geysers served as an ideal source for finding the thermophilic Actinobacteria which produced xylanase at high temperatures.

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Engineering Thermostable Microbial Xylanases Toward its Industrial Applications

Cited by 120 publications

References 88 publications

Effects of Xylanase Pretreatment on the Quality of Refiner Mechanical Mulberry Branch Fibers

Effects of Xylanase Pretreatment on the Quality of Refiner Mechanical Mulberry Branch Fibers

Screening of bacterial extracellular xylanase producers with potential for cellulose pulp biobleaching

Xylan-degrading ability of thermophilic Actinobacteria from soil in a geothermal area

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