Engineering thermotolerant Yarrowia lipolytica for sustainable biosynthesis of mannitol and fructooligosaccharides

Zhang, Yue; Zhang, Xinyue; Xu, Yanhong; Xu, Shuo; Bilal, Muhammad; Cheng, Hairong

doi:10.1016/j.bej.2022.108604

Cited by 16 publications

(15 citation statements)

References 56 publications

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“…The ratio of mannitol to erythritol reached 2.07 at 35 °C as compared to 0.269 in the cultures grown at 30 °C. The expression of YALI0C13508g and YALI0F18590g did not increase in this study 36 . Those results further support the significant role of YALIB07117g in the polyol synthesis pathway.…”

Section: Discussioncontrasting

confidence: 61%

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

Szczepańczyk

Rzechonek

Neuvéglise

et al. 2023

Sci Rep

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The unconventional yeast Yarrowia lipolytica produces erythritol as an osmoprotectant to adapt to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strains were tested for their ability to produce polyols in osmotic stress conditions. Lack of six of the reductase genes does not affect erythritol significantly, as the production of this polyol is comparable to the control strain. Deletion of eight of the homologous erythrose reductase genes resulted in a 91% decrease in erythritol synthesis, a 53% increase in mannitol synthesis, and an almost 8-fold increase in arabitol synthesis as compared to the control strain. Additionally, the utilization of glycerol was impaired in the media with induced higher osmotic pressure. The results of this research may shed new light on the production of arabitol and mannitol from glycerol by Y. lipolytica and help to develop strategies for further modification in polyol pathways in these microorganisms.

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Section: Discussioncontrasting

confidence: 61%

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

Szczepańczyk

Rzechonek

Neuvéglise

et al. 2023

Sci Rep

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show abstract

“…The ratio of mannitol to erythritol reached 2.07 at 35°C as compared to 0.269 in the cultures grown at 30°C. The expression of YALI0C13508g and YALI0F18590g did not increase in this study 37 . Those results further support the signi cant role of YALIB07117g in the polyol synthesis pathway.…”

Section: Discussioncontrasting

confidence: 61%

“…The lack of 8 putative ER genes alters the polyol production and forces the production of arabitol and mannitol. Those polyols may act as a secondary defense to osmotic stress and the pathways of their synthesis may be intertwined with erythritol production, as was previously discussed 31,37 . It is a possible outcome as mannitol is produced in response to osmotic stress as one of the main mechanisms of protection by bacteria Acinetobacter baumannii, Pseudomonas putida, and Acinetobacter baylyi [39][40][41] .…”

Section: Discussionmentioning

confidence: 84%

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

Szczepańczyk

Rzechonek

Neuvéglise

et al. 2023

Preprint

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The unconventional yeast Yarrowia lipolytica produces erythritol as a response to osmotic stress. In this study, the array of putative erythrose reductases, responsible for the conversion of d-erythrose to erythritol, was analyzed. Single knockout and multiple knockout strains were tested for their ability to produce polyols in osmotic stress conditions. Lack of six of the reductase genes does not affect erythritol significantly, as the production of this polyol is comparable to the control strain. Deletion of eight of the homologous erythrose reductase genes resulted in a 91% decrease in erythritol synthesis, a 53% increase in mannitol synthesis, and an almost 8-fold increase in arabitol synthesis as compared to the control strain. Additionally, the utilization of glycerol was impaired in the media with induced higher osmotic pressure. The results of this research may shed new light on the production of arabitol and mannitol from glycerol by Y. lipolytica and help to develop strategies for further modification in polyol pathways in these microorganisms.

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“…Above all, the strain overexpressing Hsp T.te-Gros2 demonstrated a four-fold rise in cellular growth at 42°C for 72 h compared to the control cells ( Gao et al, 2016 ). Interestingly, Y. lipolytica cells, overexpressing native Hsp90, had increased thermotolerance when cultivated at temperatures rising from 30°C to 35°C ( Zhang et al, 2022 ). However, the genes from thermophiles have not been reported to increase the thermotolerance of Y. lipolytica .…”

Section: Introductionmentioning

confidence: 99%

Enhancing the thermotolerance and erythritol production of Yarrowia lipolytica by introducing heat-resistant devices

Liang

Wang

et al. 2023

Front. Bioeng. Biotechnol.

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Yarrowia lipolytica has been widely used in the food biotech-related industry, where it plays the host’s role in producing erythritol. Nevertheless, a temperature of about 28°C–30°C has been estimated as the yeast’s optimal growth temperature, leading to the consumption of a considerable quantity of cooling water, especially in summer, which is obligatory for fermentation. Herein is described a method for improving the thermotolerance and erythritol production efficiency at high temperatures of Y. lipolytica. Through screening and testing different heat resistant devices, eight refactored engineered strains showed better growth at higher temperature and the antioxidant properties of the eight engineered strains were also improved. In addition, the erythritol titer, yield and productivity of the strain FOS11-Ctt1 represented the best among the eight strains, reaching at 39.25 g/L, 0.348 g/g glucose, and 0.55 g/L/h respectively, which were increased by 156%, 86% and 161% compared with the control strain, respectively. This study provides insight into an effective heat-resistant device that could enhance the thermotolerance and erythritol production of Y. lipolytica, which might be considered a valued scientific reference for other resistant strains’ construction.

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Engineering thermotolerant Yarrowia lipolytica for sustainable biosynthesis of mannitol and fructooligosaccharides

Cited by 16 publications

References 56 publications

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

In-depth analysis of erythrose reductase homologs in Yarrowia lipolytica

Enhancing the thermotolerance and erythritol production of Yarrowia lipolytica by introducing heat-resistant devices

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