Engineering traits through CRISPR/cas genome editing in woody species to improve forest diversity and yield

Thapliyal, Garima; Bhandari, Maneesh S.; Vemanna, Ramu S.; Pandey, Shailesh; Meena, Rajendra K.; Barthwal, Santan

doi:10.1080/07388551.2022.2092714

Cited by 12 publications

(7 citation statements)

References 177 publications

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“…One prominent challenge lies in the limited regenerative capacity observed in many forest tree species. Additionally, the long growth cycle of forest trees further complicates the timeline required for achieving mature transgenic individuals [4]. These factors contribute to the substantial temporal and financial investments associated with research in forest genetic transformation.…”

Section: Advancements In Tree Genetic Transformation Processmentioning

confidence: 99%

“…Traditional breeding methods are inadequate to cope with escalating environmental pressures and the intricate challenges posed by pests and diseases. Moreover, the genetic improvement of trees is constrained by issues such as long-life cycles, long generation times, late sexual maturity and limited genetic diversity [3,4]. Therefore, tree genetic transformation has become a focal point of intensive research.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Li,

Yuan,

et al. 2024

Forests

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Forests represent a vital natural resource and play a crucial role in climate regulation and maintaining biodiversity. However, the growth and development of forest trees are increasingly challenged by rising environmental pressures, particularly detrimental abiotic stressors. To address these challenges, genetic transformation technologies have emerged as effective solutions. Despite various difficulties in genetic transformation for forest trees, including prolonged life cycles, genetic diversity, interspecies variations, and complex regeneration systems, significant research progress has been achieved in tree gene editing, transgenic technology, and methods for delivering exogenous molecules. These technologies have the potential to enhance tree quality, increase productivity, and improve resistance to abiotic stress. This review provides an overview of the main methods and transformation receptors in tree genetic transformation. Additionally, we summarize several novel techniques, such as nanoparticle-mediated gene transformation, advanced gene editing technology, various novel delivery carriers, and non-genetically modified protein function interference through peptide aptamer. Notably, we also place emphasis on several referable genes from forest trees and common crops, together with their potential function for improving abiotic stress responses. Through this research, we aspire to achieve sustainable utilization and conservation of tree resources, thereby providing substantial support for future livelihoods and economic development.

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Section: Advancements In Tree Genetic Transformation Processmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Li,

Yuan,

et al. 2024

Forests

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“… 7 Due to the development of this technique, Emmanuel Charpentier and Jennifer Doudna received the Nobel Prize in 2020. 8 , 9 …”

Section: Introductionmentioning

confidence: 99%

Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine

Khoshandam¹,

Soltaninejad²,

Mousazadeh³

et al. 2024

Genes & Diseases

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“…Furthermore, researchers are exploring new ways to use CRISPR to introduce new traits that reduce the need of pesticides, increase wood production, improve wood quality, and increase the sustainability of forestry operations. 30 , 31 Here this review will focus on the acceleration of plant breeding through gene editing strategy of CRISPR/Cas9 RNA-guided endonuclease (RGEN) system and how it is helpful to accelerate molecular plant breeding for crop improvement program.…”

Section: Introduction/basic Gene Editing Strategymentioning

confidence: 99%

CRISPR/Cas9-gene editing approaches in plant breeding

Saini,

Thakur,

Gill

et al. 2023

GM Crops & Food

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CRISPR/Cas9 gene editing system is recently developed robust genome editing technology for accelerating plant breeding. Various modifications of this editing system have been established for adaptability in plant varieties as well as for its improved efficiency and portability. This review provides an in-depth look at the various strategies for synthesizing gRNAs for efficient delivery in plant cells, including chemical synthesis and in vitro transcription. It also covers traditional analytical tools and emerging developments in detection methods to analyze CRISPR/Cas9 mediated mutation in plant breeding. Additionally, the review outlines the various analytical tools which are used to detect and analyze CRISPR/Cas9 mediated mutations, such as next-generation sequencing, restriction enzyme analysis, and southern blotting. Finally, the review discusses emerging detection methods, including digital PCR and qPCR. Hence, CRISPR/Cas9 has great potential for transforming agriculture and opening avenues for new advancements in the system for gene editing in plants.

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Engineering traits through CRISPR/cas genome editing in woody species to improve forest diversity and yield

Cited by 12 publications

References 177 publications

Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Genetic Transformation of Forest Trees and Its Research Advances in Stress Tolerance

Clinical applications of the CRISPR/Cas9 genome-editing system: Delivery options and challenges in precision medicine

CRISPR/Cas9-gene editing approaches in plant breeding

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