Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants

Liu, Changjiang; Yu, Huan; Zhang, Baocai; Liu, Shilin; Liu, Chenguang; Li, Feng; Song, Hao

doi:10.1016/j.biotechadv.2022.108019

Cited by 68 publications

(32 citation statements)

References 141 publications

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“… 27 However, colorimetric analysis facilitates the development of a mini-equipment biosensor that can be read directly by the naked eye. 9 Thus, the crtY gene encoding lycopene cyclase, which transforms lycopene into more stable β-carotene, was introduced into the reporter system ( Fig. 1C ).…”

Section: Resultsmentioning

confidence: 99%

“…6 As a supplement to instrumental methods, various programmed whole-cell biosensors have been artificially designed to focus on detecting bioavailable heavy metals, including Hg( ii ), by simulating environmental microbes for their ecological risk prediction. 7–9…”

Section: Introductionmentioning

confidence: 99%

“…6 As a supplement to instrumental methods, various programmed whole-cell biosensors have been articially designed to focus on detecting bioavailable heavy metals, including Hg(II), by simulating environmental microbes for their ecological risk prediction. [7][8][9] Whole-cell biosensors consist of a sensor and an actuator, allowing them to monitor environmental heavy metals using colorimetric, electrochemical, uorescent, or luminescent output signals. 10,11 Among traditional reporters, colorimetric rainbow colorants have recently been inuential in developing miniequipment, low-cost, and portable whole-cell biosensors for toxic metals.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor

Hui¹,

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et al. 2022

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor

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Li³

et al. 2022

RSC Adv.

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“…In the classic method of constructing a WCB, the responsive genes plus promoter or only the promoter itself is fused to a reporter gene without its promoter. The fused gene elements can be constructed on a plasmid and then the recombinant plasmid can be transformed into a host cell (Gupta et al, 2019;Liu et al, 2022). When applied to the detection of target compounds or stress factors, the transcriptional regulators in these WCBs stimulate the promoters, which leads to the expression of downstream reporter genes and the increase of the output signal for Classes I and II (Shin, 2011).…”

Section: Regulatory Genesmentioning

confidence: 99%

“…The main mechanism of how regulatory proteins work involves an effector-binding response to chemical species with structures that enable cognate recognition (Bilal and Iqbal, 2019;Liu et al, 2022). In some cases, targets with structural similarity may all provoke a response.…”

Section: Regulatory Genesmentioning

confidence: 99%

Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems

et al. 2022

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Water contamination has become increasingly a critical global environmental issue that threatens human and ecosystems’ health. Monitoring and risk assessment of toxic pollutants in water bodies is essential to identifying water pollution treatment needs. Compared with the traditional monitoring approaches, environmental biosensing via whole-cell bioreporters (WCBs) has exhibited excellent capabilities for detecting bioavailability of multiple pollutants by providing a fast, simple, versatile and economical way for environmental risk assessment. The performance of WCBs is determined by its elements of construction, such as host strain, regulatory and reporter genes, as well as experimental conditions. Previously, numerous studies have focused on the design and construction of WCB rather than improving the detection process and commercialization of this technology. For investigators working in the environmental field, WCB can be used to detect pollutants is more important than how they are constructed. This work provides a review of the development of WCBs and a brief introduction to genetic construction strategies and aims to summarize key studies on the application of WCB technology in detection of water contaminants, including organic pollutants and heavy metals. In addition, the current status of commercialization of WCBs is highlighted.

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Biosyncretic Robots Actuated by Living Materials

Yang,

Zhang,

Wang

et al. 2023

Adv Materials Technologies

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In recent years, biosyncretic robots, a novel type of robot that integrates living and artificial materials, have gained significant attention. Biosyncretic robots are expected to leverage the advantages provided by living materials and offer a new approach to address the challenges faced by traditional robots, such as biocompatibility, intrinsic safety, and miniaturization. Therefore, this review aims to provide an overview of the current state of development of biosyncretic robots. First, the existing research on biosyncretic robots based on commonly used living materials is systematically categorized and introduced, including cardiomyocytes, skeletal muscle cells, insect dorsal vascular tissue, and microorganisms. Subsequently, their potential in the fields of in vivo medical diagnosis and treatment, organ‐on‐a‐chip, tissue engineering, environmental monitoring, and postdisaster rescue in detail is also discussed. Finally, the current challenges faced by biosyncretic robotic research, including establishing theoretical models, fabrication, cultivation, and maintenance of living materials, controllability, versatility, artificial material, and entering the human body or leaving the laboratory, are examined. This review summarizes the cutting‐edge progress of biosyncretic robots and provides insights into their future development.

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Engineering whole-cell microbial biosensors: Design principles and applications in monitoring and treatment of heavy metals and organic pollutants

Cited by 68 publications

References 141 publications

Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor

Metabolic engineering of the carotenoid biosynthetic pathway toward a specific and sensitive inorganic mercury biosensor

Use of whole-cell bioreporters to assess bioavailability of contaminants in aquatic systems

Biosyncretic Robots Actuated by Living Materials

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