Engraftment of neural stem cells in the treatment of spinal cord injury

Mortazavi, Martin M.; Jaber, Mohammad; Adeeb, Nimer; Deep, Aman; Hose, Nicole; Rezaei, M.; Fard, Salman Abbasi; Kateb, Babak; Yashar, Parham; Liker, Mark A.; Tubbs, R. Shane

doi:10.1016/j.tria.2015.10.002

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…15-40/million people suffer from SCI each year, WHO estimates only 250,000 to 500,000 people suffer SCI each year, including approximately 12,000 cases in the United States. (Mortazavi et al, 2015;One Degree, 2009 SCI include vehicle accidents, falls, sports and other trauma, infections and tumors. The majority of SCI victims are young patients, who are at the prime of their working age (Tator, 1995).…”

Section: Introductionmentioning

confidence: 99%

Stem cell based therapies for spinal cord injury

Muheremu

Jiang²,

2016

Tissue and Cell

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Section: Introductionmentioning

confidence: 99%

Stem cell based therapies for spinal cord injury

Muheremu

Jiang²,

2016

Tissue and Cell

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“…The traditional cell-based approach for neuroregeneration in CNS has been engrafting neural stem cells. However, this procedure often leads to immune rejection of transplanted externally reprogrammed cells and poor functional integration into the host tissue [ 8 , 9 ]. Alternative cell-based approaches include in vivo reprogramming of endogenous glial cells into neurons avoiding adverse immune responses.…”

Section: Introductionmentioning

confidence: 99%

Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons

Mungenast¹,

Nieminen²,

Gaiser³

et al. 2023

Biomaterials and Biosystems

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“…Cell transplantations have shown initial promise both broadly in tissue engineering (Kwon et al, 2018) and specifically in SCI repair models (Dulin & Lu, 2014). On a cellular level, neural stem cell (NSC) transplants repopulate lost neural and glial cells in damaged tissue (Dulin & Lu, 2014;Mortazavi et al, 2015) in addition to working on a molecular level through cytokine and chemokine release post-SCI (P. Lu et al, 2003). Furthermore, transplanted NSCs can integrate with endogenous neural circuitry (Ceto et al, 2020;Kumamaru et al, 2018) and can significantly decrease local expression of pro-inflammatory cytokines (Cheng et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

IL‐10 lentivirus‐laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury

Ciciriello

Smith

Munsell

et al. 2021

Biotech & Bioengineering

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A complex cellular cascade characterizes the pathophysiological response following spinal cord injury (SCI) limiting regeneration. Biomaterial and stem cell combination therapies together have shown synergistic effects, compared to the independent benefits of each intervention, and represent a promising approach towards regaining function after injury. In this study, we combine our polyethylene glycol (PEG) cell delivery platform with lentiviral-mediated overexpression of the antiinflammatory cytokine interleukin (IL)−10 to improve mouse embryonic Day 14 (E14) spinal progenitor transplant survival. Immediately following injury in a mouse SCI hemisection model, five PEG tubes were implanted followed by direct injection into the tubes of lentivirus encoding for IL-10. Two weeks after tube implantation, mouse E14 spinal progenitors were injected directly into the integrated tubes, which served as a soft substrate for cell transplantation. Together, the tubes with the IL-10 encoding lentivirus improved E14 spinal progenitor survival, assessed at 2 weeks posttransplantation (4 weeks postinjury). On average, 8.1% of E14 spinal progenitors survived in mice receiving IL-10 lentivirus-laden tubes compared with 0.7% in mice receiving transplants without tubes, an 11.5-fold difference. Surviving E14 spinal progenitors gave rise to neurons when injected into tubes. Axon elongation and remyelination were observed, in addition to a significant increase in functional recovery in mice receiving IL-10 lentivirus-laden tubes with E14 spinal progenitor delivery compared to the injury only control by 4 weeks postinjury. All other conditions did not exhibit increased stepping until 8 or 12 weeks postinjury.This system affords increased control over the transplantation microenvironment, offering the potential to improve stem cell-mediated tissue regeneration.

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Engraftment of neural stem cells in the treatment of spinal cord injury

Cited by 5 publications

References 47 publications

Stem cell based therapies for spinal cord injury

Stem cell based therapies for spinal cord injury

Electrospun decellularized extracellular matrix scaffolds promote the regeneration of injured neurons

IL‐10 lentivirus‐laden hydrogel tubes increase spinal progenitor survival and neuronal differentiation after spinal cord injury

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