Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44

Zhang, Jian; Caiyin, Qinggele; Feng, Wenjing; Zhao, Xueping; Qiao, Bin; Zhao, Guang‐Rong; Qiao, Jianjun

doi:10.1038/srep27973

Cited by 49 publications

(40 citation statements)

References 64 publications

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“…Nisin stock was prepared in 10 mM HEPES (pH–7.4) containing 150 mM NaCl and subsequently filtered through 0.22 μm syringe filter prior to use6263. For fluorescence measurements nisin stock was prepared in 280 mM sucrose supplemented with 5 mM Tris (pH 7.5) buffer.…”

Section: Methodsmentioning

confidence: 99%

Lipid-II Independent Antimicrobial Mechanism of Nisin Depends On Its Crowding And Degree Of Oligomerization

Prince

Sandhu

Ror

et al. 2016

Sci Rep

116

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Nisin inhibits bacterial growth by generating pores in cell membrane and interrupting cell-wall biosynthesis through specific lipid II interaction. However, the role of the hinge region and C-terminus residues of the peptide in antibacterial action of nisin is largely unknown. Here, using molecular dynamics simulations and experimental approach, we report that at high concentration regimes of nisin, interaction with phospholipids may equally deform the bacterial cell membranes even under significantly varying amounts of lipid-II. Membrane thinning, destabilization and decrease in lipid density depend on the degree of oligomerization of nisin. Growth kinetics of Bacillus subtilis and Escherichia coli interestingly show recovery by extended lag phase under low concentrations of nisin treatment while high concentrations of nisin caused decrease in cell viability as recorded by striking reduction in membrane potential and surface area. The significant changes in the dipole potential and fluorescence anisotropy were observed in negatively charged membranes in the absence of lipid-II with increasing concentration of nisin. The identical correlation of cell viability, membrane potential dissipation and morphology with the concentration regime of nisin, in both Bacillus subtilis (lipid II rich) and Escherichia coli (lipid II impoverished), hints at a non-specific physical mechanism where degree of membrane deformation depends on degree of crowding and oligomerization of nisin.

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Section: Methodsmentioning

confidence: 99%

Lipid-II Independent Antimicrobial Mechanism of Nisin Depends On Its Crowding And Degree Of Oligomerization

Prince

Sandhu

Ror

et al. 2016

Sci Rep

116

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“…lactis F44 was obtained by the genome shuffling of L. lactis ssp. lactis YF11 (accession number CGMCC7.52) in our previous study (Zhang et al, 2016). The inoculum was prepared at 30°C for 8 h (optical density at 600 nm ~1.5) in seed medium and was inoculated (10%) into the fresh fermentation medium with different 12 stress conditions: 1% lactic acid with pH 4.0, pH 5.0, pH 6.0, or pH 7.0; 2% lactic acid with pH 4.0, pH 5.0, pH 6.0, or pH 7.0; or 3% lactic acid with pH 4.0, pH 5.0, pH 6.0, or pH 7.0.…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

“…On the other hand, 2 ribosomal proteins, RplM and RpsS, were increased in Lactobacillus bulgaricus CAUH1 under acid stress . The genes encoding ribosomal proteins L31 and L25 were also associated with stress resistance in B. subtilis Zhang et al, 2016). Furthermore, these proteins not only constitute the ribosomal subunits related to translation, but also participate in sensing environmental change (VanBogelen and Neidhardt, 1990;Wehmeier et al, 2001;.…”

Section: Cross-stress Affects the Biosynthesis Of Ribosomal Proteins mentioning

confidence: 99%

Quantitative proteomics of Lactococcus lactis F44 under cross-stress of low pH and lactate

Zhao

Du³

et al. 2018

Journal of Dairy Science

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Lactococcus lactis encounters 3 environmental stimuli, H, lactate, and undissociated lactic acid, because of the accumulation of lactic acid-the predominant fermentation product. Few studies have examined how these stimuli synergistically affect the bacteria. Herein, we analyzed the dissociation degree of lactic acid at different pH and investigated the cellular response to cross-stress in L. lactis ssp. lactis F44 through quantitative proteomic analysis using isobaric tags for relative and absolute quantitation of 3 groups: 0% lactic acid with pH 4.0 and 0% lactic acid with pH 5.0 for acid stress; 2% lactic acid with pH 7.0 and 3% lactic acid with pH 7.0 for lactate stress; and 2% lactic acid with pH 4.0, 2% lactic acid with pH 5.0, 3% lactic acid with pH 4.0, and 3% lactic acid with pH 5.0 for cross-stress. We observed that the metabolisms of carbohydrate and energy were inhibited, which might be due to the feedback inhibition of lactic acid. The arginine deiminase pathway was improved to maintain the stability of intracellular pH. Additionally, some differentially expressed genes associated with the general stress response, amino acid metabolism, cell wall synthesis, and regulatory systems played significant roles in stress response. Overall, we highlighted the response mechanisms to lactic acid stress and provided a new opportunity for constructing robust industrial strains.

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“…It has also been approved as a food additive (E234) in the EU, according to Directive 95/2/EC (EC, 1995) [141]. Nisin production in L. lactis has been enhanced [142] and its bactericidal effects increased through genetic engineering of the strain [143].…”

Section: Genetically Modified Lactic Acid Bacteria As Cell Factoriesmentioning

confidence: 99%

Safety Aspects of Genetically Modified Lactic Acid Bacteria

Plavec

Berlec

2020

Microorganisms

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Lactic acid bacteria (LAB) have a long history of use in the food industry. Some species are part of the normal human microbiota and have beneficial properties for human health. Their long-standing use and considerable biotechnological potential have led to the development of various systems for their engineering. Together with novel approaches such as CRISPR-Cas, the established systems for engineering now allow significant improvements to LAB strains. Nevertheless, genetically modified LAB (GM-LAB) still encounter disapproval and are under extensive regulatory requirements. This review presents data on the prospects for LAB to obtain ‘generally recognized as safe’ (GRAS) status. Genetic modification of LAB is discussed, together with problems that can arise from their engineering, including their dissemination into the environment and the spread of antibiotic resistance markers. Possible solutions that would allow the use of GM-LAB are described, such as biocontainment, alternative selection markers, and use of homologous DNA. The use of GM-LAB as cell factories in closed systems that prevent their environmental release is the least problematic aspect, and this is also discussed.

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Enhance nisin yield via improving acid-tolerant capability of Lactococcus lactis F44

Cited by 49 publications

References 64 publications

Lipid-II Independent Antimicrobial Mechanism of Nisin Depends On Its Crowding And Degree Of Oligomerization

Lipid-II Independent Antimicrobial Mechanism of Nisin Depends On Its Crowding And Degree Of Oligomerization

Quantitative proteomics of Lactococcus lactis F44 under cross-stress of low pH and lactate

Safety Aspects of Genetically Modified Lactic Acid Bacteria

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