2014
DOI: 10.2323/jgam.60.183
|View full text |Cite
|
Sign up to set email alerts
|

Enhanced 3-methylcatechol production by Pseudomonas putida TODE1 in a two-phase biotransformation system

Abstract: In this study, genetically engineered Pseudomonas putida TODE1 served as a biocatalyst for the bioproduction of valuable 3-methylcatechol (3MC) from toluene in an aqueous-organic two-phase system. The two-phase system was used as an approach to increase the biocatalyst efficiency. Among the organic solvent tested, n-decanol offered several benefits including having the highest partitioning of 3MC, with a high 3MC yield and low cell toxicity. The effect of media supplementation with carbon/ energy sources (gluc… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
1
0
1

Year Published

2016
2016
2023
2023

Publication Types

Select...
2
1

Relationship

0
3

Authors

Journals

citations
Cited by 3 publications
(2 citation statements)
references
References 25 publications
0
1
0
1
Order By: Relevance
“…Toluene and its derivatives (i.e., chloro-toluenes and xylenes) are toxic chemicals that can be used as raw materials for the biosynthesis of other aromatic compounds of industrial interest, such as methyl-and chlorocatechols, substituted semialdehydes, p-hydroxybenzoate, and others (Neumann et al, 2004;Verhoef et al, 2010;Kongpol et al, 2014;Verhoef et al, 2014). Biosynthesis requires that the metabolic potential of the strains, as well as their tolerance to toxic chemicals are taken into account.…”
Section: Introductionmentioning
confidence: 99%
“…Toluene and its derivatives (i.e., chloro-toluenes and xylenes) are toxic chemicals that can be used as raw materials for the biosynthesis of other aromatic compounds of industrial interest, such as methyl-and chlorocatechols, substituted semialdehydes, p-hydroxybenzoate, and others (Neumann et al, 2004;Verhoef et al, 2010;Kongpol et al, 2014;Verhoef et al, 2014). Biosynthesis requires that the metabolic potential of the strains, as well as their tolerance to toxic chemicals are taken into account.…”
Section: Introductionmentioning
confidence: 99%
“…Pseudomonas (FAIZAL et al, 2007;HELD et al, 1999;HÜSKEN et al, 2002;KONGPOL et al, 2014;SHIRAI, 1986;WERY;MENDES DA SILVA;DE BONT, 2000). Os metagenomas de P1-2013 e P2-2013 mostraram a mesma fenol hidrolase (K16242) mencionada no catabolismo de fenol (Figura 18), transformando tolueno em 3MC através da via do orto-cresol (Figura 19A), a qual já foi utilizada com uma 2,3dihidroxi-bifenil 1,2-dioxigenase de E. coli recombinantes para produção de 3MC (SHI et al, 2013).…”
Section: Potencial Para Degradação De Compostos Aromáticosunclassified