Enhanced [3H] Glutamate Binding in the Cerebellum of Insulin-Induced Hypoglycaemic and Streptozotocin-Induced Diabetic Rats

Joseph, Abin John; Robinson, Remya; Paulose, C S

doi:10.1007/s10571-007-9198-1

Cited by 9 publications

(2 citation statements)

References 67 publications

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“…In our earlier studies, we reported the glutamate mediated excitotoxicity in the cerebellum of insulin induced hypoglycemic and streptozotocin induced diabetic rats [32]. In the present study, we have demonstrated the role of cholinergic receptors during recurrent hypoglycemia in diabetic rats.…”

Section: Discussionsupporting

confidence: 68%

“…In a case of episodic bilateral cerebellar dysfunction caused by hypoglycemia, quantitative dynamic PET study demonstrated decreased glucose uptake-to-utilization ratio and increased leak of glucose in the cerebellum indicating that cerebellum is not invariably resistant to hypoglycemia [31]. Studies from our laboratory have demonstrated that cerebellum is susceptible to hypoglycemia [32,33]. Studies on damages of the central nervous system under conditions of hypoglycemia are very important for clinical medicine.…”

Section: Introductionmentioning

confidence: 99%

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Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic

Sasoh

Kawanishi

et al. 2010

J Biomed Sci

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BackgroundArsenic exposure induces overproduction of reactive nitrogen species (RNS) in brain tissue and results in nucleic acid damage to the nerve cells. The 8-nitroguanine is one of the major products formed by the reaction of guanine, and ONOO-, and has been used as a popular biomarker of nucleic acid damage due to RNS attacking. In the present study, we examined whether the administration of taurine can protect against nucleic acid damage of brain neurons by arsenic-induced RNS.Materials and methodsSixty mice (30 male and 30 female) weighing 19.5 ± 1.5 g were divided into 3 groups: (1) control group, (2) experimental group that received arsenic (As2O3), and (3) antagonistic group that received taurine with arsenic. Arsenic was administered for 60 days. 8-Nitroguanine expressions in brain neurons of mice were examined by the immunohistochemical method. Histopathological changes in brain tissues of mice were observed under light microscope and the immunohistochemistry method was used to investigate 8-nitroguanine expressions in cerebrum and cerebellum of mice.ResultsIn the control group, no abnormal histopathological changes were observed in brain tissue of the mice. In brain tissue of the mice exposed to arsenic, histopathological results showed swells, evident vacuolar degeneration in cytoplasm, karyorrhexis and karyolysis. Relatively light pathological changes were observed in brain of the mice co-administered arsenic and taurine. Little or no expression of 8-nitroguanine in brain tissue was observed in controls. However, intensive expression of 8-nitroguanine was found in brain tissue of mice exposed to arsenic and it was mainly distributed in nucleus neighbouring the nuclear membrane, but a little in cytoplasm. A weak expression of 8-nitroguanine was observed in brain cells of mice co-administered arsenic and taurine.ConclusionsThe brain neurons may be the major target cells of arsenic neurotoxicity. Co-administration of arsenic and taurine can alleviate DNA damage of brain neurons caused by arsenic through the RNS signal pathway.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic

Sasoh

Kawanishi

et al. 2010

J Biomed Sci

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Enhanced Dopamine D1 and D2 Receptor Gene Expression in the Hippocampus of Hypoglycaemic and Diabetic Rats

2009

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Hypoglycaemic coma and brain injury are potential complications of insulin therapy. Hippocampal neurons are particularly vulnerable to hypoglycaemic stress leading to memory impairment. In the present article, we have investigated the dopamine (DA) content, homovanillic acid (HVA)/DA turnover ratio, DA D(1) and DA D(2) receptors in the hippocampus of insulin-induced hypoglycaemic (IIH) and streptozotocin induced diabetic rats where brain functions are impaired. The DA content decreased significantly in hippocampus of diabetic, diabetic +IIH and control +IIH rats compared to control. The HVA/DA turnover ratio also increased significantly in diabetic, diabetic +IIH and control +IIH rats compared to control. Scatchard analysis using [(3)H] DA in the hippocampus showed a significant increase in DA receptors of diabetic, diabetic +IIH and control +IIH rats with decreased affinity. Gene expression studies using Real-time PCR showed an increased expression of DA D(1) and DA D(2) receptors in the hippocampus of hypoglycaemic and diabetic rats. Our results indicate that the dopaminergic system is impaired in the hippocampus of hypoglycaemic and hyperglycaemic rats impairing DA related functions of hippocampus. We observed a prominent dopaminergic functional disturbance in the hypoglycaemic condition than in hyperglycaemia compared to control. This dopaminergic dysfunction in hippocampus during hypoglycaemia and hyperglycaemia is suggested to contribute to cognitive and memory deficits. This will have clinical significance in the treatment of diabetes.

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Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability

Zhou,

Xie,

Kong

et al. 2024

Biochemical Pharmacology

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Enhanced [3H] Glutamate Binding in the Cerebellum of Insulin-Induced Hypoglycaemic and Streptozotocin-Induced Diabetic Rats

Cited by 9 publications

References 67 publications

Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic

Protection effect of taurine on nitrosative stress in the mice brain with chronic exposure to arsenic

Enhanced Dopamine D1 and D2 Receptor Gene Expression in the Hippocampus of Hypoglycaemic and Diabetic Rats

Regulatory roles of histamine receptor in astrocytic glutamate clearance under conditions of increased glucose variability

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